| recent advances in the aetiology of viral gastroenteritis. | studies with the human reovirus-like (hrvl) agnet, also designated rotavirus and duovirus, have revealed that it is a major aetiological agent of diarrhoea of infants and young children in many parts of the world. in a study of patients admitted with a diarrhoeal illness to the children's hospital of the district of columbia in the united states from january 1974 to june 1975, it was found that half of the patients studied by both virus shedding (by electron microscopy) and serological (compleme ... | 1976 | 186239 |
| epidemiology of human rotavirus types 1 and 2 as studied by enzyme-linked immunosorbent assay. | to determine the relative importance of two known serotypes of human rotavirus, we developed an enzyme-linked immunosorbent assay to differentiate serotype-specific rotavirus antigen and antibody. using this technic, we studied the epidemiology of the two serotypes in acute gastroenteritis. seventy-seven per cent of 414 rotavirus isolates were type 2, and the remainder were type 1. the serotype distribution was similar in specimens from children in washington, d.c., and other parts of the world. ... | 1978 | 212678 |
| comparative epidemiology of two rotavirus serotypes and other viral agents associated with pediatric gastroenteritis. | human rotavirus (hrv) type 1 or 2, adenovirus, or non-cultivatable 27 nm virus-like particles were demonstrated by electron microscopy and/or rotavirus elisa in fecal samples from 45.5% of 604 gastroenteritis inpatients, 25.0% of 200 gastroenteritis outpatients and 6.0% of 812 control subjects, all sampled at children's hospital national medical center. washington, dc. rotaviruses were the most common pathogens detected as 39% and 22% of gastroenteritis inpatients and outpatients, respectively, ... | 1979 | 224698 |
| fecal adenoviruses from a longitudinal study of families in metropolitan washington, d.c.: laboratory, clinical, and epidemiologic observations. | during a 29-month period, we studied enteric infection in 70 families from a pediatric practice in suburban washington, d.c. fecal adenoviruses were detected in stools of 18 patients by tissue culture and electron microscopic procedures. from 6 through 11 months of age, the incidence of fecal adenoviruses associated with enteritis was seven per 100, and of confirmed enteric adenoviruses (eads), three per 100 individuals per year. all eads belonged to subgenus g (type 41). all three patients with ... | 1985 | 2995630 |
| rotavirus gastroenteritis in the washington, dc, area: incidence of cases resulting in admission to the hospital. | the incidence of rotavirus gastroenteritis in infants and children that required admission to the hospital was estimated for a defined population of approximately 105,000 individuals, including 29,000 children aged 15 years or younger whose primary health care was provided by group health association, inc, a health maintenance organization in the washington, dc, area. from january 1977 through march 1979, almost all infants and children in this age group who required hospitalization for gastroen ... | 1980 | 6250399 |
| approaches to immunization of infants and young children against gastroenteritis due to rotaviruses. | recent studies have shown that in developed countries rotaviruses are the single most important etiologic agents of acute gastroenteritis that requires hospitalization of infants and young children. although deaths from gastroenteritis are, in general, infrequent in the developed countries, an effective rotavirus vaccine would clearly be of benefit to reduce the heavy toll of morbidity from gastroenteritis due to rotavirus. in the developing countries the impact of diarrheal diseases is staggeri ... | 1980 | 6251528 |
| pediatric viral gastroenteritis during eight years of study. | during the period january 1974 through july 1982, fecal samples from 1,537 pediatric inpatients with gastroenteritis were tested for enteric viruses by electron microscopic and rotavirus enzyme-linked immunosorbent assay techniques. rotaviruses were detected in 34.5% of these patients, enteric adenoviruses were detected in 4.7%, approximately 27-nm viruses were detected in 1.6%, and at least one of these agents was found in 40.1% of the study subjects. three infections were by an apparently new ... | 1983 | 6309901 |
| use of electrophoresis of rna from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery. | during two nosocomial outbreaks in a tertiary care nursery, 22 of 102 infants had infections due to human rotavirus (hrv). the time sequence and proximity of the infected infants in the nursery rooms had suggested infant-to-infant spread of a single hrv strain in each outbreak. polyacrylamide gel electrophoresis of virus permitted demonstration of four different hrv electropherotypes (strains) during the first outbreak period and seven during the second. one of the strains was detected during bo ... | 1983 | 6309996 |
| foodborne outbreak of group a rotavirus gastroenteritis among college students--district of columbia, march-april 2000. | on march 31, student health services at a university in the district of columbia (dc) notified the dc health department that an increased number of students had become ill with acute gastroenteritis beginning march 29. some ill students reported eating tuna or chicken salad sandwiches from dining hall a on campus. on march 31, the dc health department initiated an outbreak investigation. this report summarizes results of the investigation, which indicated that group a rotavirus transmitted by fo ... | 2000 | 11190116 |
| from the centers for disease control and prevention. foodborne outbreak of group a rotavirus gastroenteritis among college students--district of columbia, march-april 2000. | | 2001 | 11263404 |
| outbreaks of adult gastroenteritis traced to a single genotype of rotavirus. | between november 1998 and december 2000, the centers for disease control and prevention screened samples from 263 outbreaks of gastroenteritis in the united states and identified 3 that were associated with rotavirus among adults. rotaviruses from each outbreak were further characterized by reverse-transcription polymerase chain reaction. surprisingly, all specimens were of serotype g2, a strain that is, as determined by high-stringency hybridization analysis, genetically distinct in all 11 gene ... | 2002 | 11992287 |
| the vp7 genes of two g9 rotaviruses isolated in 1980 from diarrheal stool samples collected in washington, dc, are unique molecularly and serotypically. | in a retrospective study of archival diarrheal stool samples collected from 1974 to 1991 at children's hospital national medical center, washington, dc, we detected three genotype g9p[8] viruses in specimens collected in 1980, which represented the earliest human g9 viruses ever isolated. the vp7 genes of two culture-adapted 1980 g9 viruses were phylogenetically related closely to the lineage 2 g9 virus vp7 gene. unexpectedly, however, the vp7s of the 1980 g9 viruses were more closely related se ... | 2008 | 18234798 |
| a critique of criteria for evaluating vaccines for inclusion in mandatory school immunization programs. | several new vaccines for children and young adults have been introduced recently and now appear on the advisory committee on immunization practices' recommended childhood and adolescent immunization schedule (meningococcal, rotavirus, human papillomavirus). as new vaccines are introduced, states face complex decisions regarding which vaccines to fund and which vaccines to require for school or child care entry. this complexity is evidenced by the current debate surrounding the human papillomavir ... | 2008 | 18676536 |
| intra-genotypic diversity of archival g4p[8] human rotaviruses from washington, dc. | group a human rotaviruses (rvs) remain the most frequently detected viral agents associated with acute gastroenteritis in infants and young children. despite their medical importance, relatively few complete genome sequences have been determined for commonly circulating g/p-type strains (i.e., g1p[8], g2p[4], g3p[8], g4p[8], and g9p[8]). in the current study, we sequenced the genomes of 11 g4p[8] isolates from stool specimens that were collected in washington, dc during the years of 1974-1991. w ... | 2011 | 21712102 |