Publications
| increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (rt-pcr) assay in stool specimens from children with diarrhea. | six-hundred and twenty-six stool specimens collected from children with diarrhea over a 12-month period were tested for rotavirus using a real time reverse transcription-polymerase chain reaction (rt-pcr) assay, a conventional nested pcr assay and by electron microscopy (em). a fragment of 87 bp in a highly-conserved region of non-structural protein 3 (nsp3) in rotavirus genome was amplified by a single-step rt-pcr protocol in a closed-tube system. rotavirus was detected in 123 samples (20%) wit ... | 2004 | 14748075 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |