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direct identification of serotypes of natural human rotavirus isolates by hybridization using cdna probes derived from segment 9 of the rotavirus genome.under stringent hybridization conditions, cdna of segment 9 of the rotavirus genome, which codes for the viral protein vp7, permitted differentiation of serotypes of culture-grown rotaviruses and natural isolates of human rotaviruses directly from clinical specimens. this was evident for the following reasons. (i) the cdna of one serotype selectively hybridized with the rna of the same serotype of culture-grown rotaviruses. (ii) natural isolates of the virus thus identified as serotype 2 were al ...19892541169
occurrence of rotaviruses in guangzhou and hong kong.electropherotypes of human rotavirus (hrv) isolated from hong kong and guangzhou were compared to study the transmission of hrv between the cities. forty-one electropherotypes were identified from 418 hospitalized patients with hrv infection at the queen mary hospital, and 22 were detected from 66 outpatients in community clinics at a separate area in hong kong. nineteen electropherotypes were detected in 370 hospitalized cases of hrv infection from seven hospitals in guangzhou. results showed t ...19882826616
[investigation of group a rotavirus infection in several cities in china and prevalence of vp7 serotype].to study the prevalence of group a rotavirus infection in china.200112526310
surveillance and risk factors of norovirus gastroenteritis among children in a southern city of china in the fall-winter seasons of 2003-2006.noroviruses (novs) are an important cause of acute gastroenteritis but knowledge on the disease burden and epidemiology in children in the developing countries remains limited. in this study, we performed a surveillance of nov gastroenteritis in children of china to address some of the questions.201019943862
[pathogens of high incidence of other infectious diarrhea in guangdong province, from november 2008 to january 2009].from november 2008 to january 2009, a sharp increase of diarrhea in children in guangdong province appeared, we randomly collected 53 stool specimens from out-patient children with dirrhea in 3 major hospitals (guangzhou city children's hospital, shenzhen baoan district maternal and child health hospital, first affiliated hospital of shantou university). rotavirus and calicivirus were screened by elisa and rt-pcr. we found 29 cases of rotavirus infection with diverse serotypes. only four cases w ...201020480645
[epidemiological surveillance of norovirus and rotavirus diarrhea among outpatient children in five metropolitan cities].to survey the clinical epidemiological features of norovirus and rotavirus diarrhea among children living in 5 cities.201021055297
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