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[detection of hepatitis g virus infection among clinical patients with hepatitis/liver diseases in china by reverse transcription-nested polymerase chain reaction].according to two highly conserved genome sequences within the helicase(ns3) region and 5'-uncoding(5' utr) region, we designed two sets of primer pairs to detect hgv rna by rt-nested pcr in order to study hgv infection in chinese population. three hundred and fifty-four serum specimens of various liver diseases were collected from beijing, qin huangdao and henan areas. seventy-nine out of 354(22.3%) specimens were hgv rna positive. among 254 known clinical hepatitis/liver disease samples, 50(19. ...199812515201
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