microbiological findings in dogs with diarrhoea. 19817340092
are the birds feeding you campylobacter?during may and june 1992 there was a marked increase in reports of campylobacter infections compared with other years. several affected patients mentioned they had been drinking milk from bottles previously pecked by birds. a case control study was initiated to investigate whether there was any significant association. it was concluded that drinking milk pecked by birds was associated significantly with subsequent campylobacter infection.19938303794
frequency of occurrence of campylobacter spp. in red meats and poultry in northern ireland and their subsequent subtyping using polymerase chain reaction-restriction fragment length polymorphism and the random amplified polymorphic dna method.sampling of lamb (n = 100) and beef (n = 100) carcasses in abattoirs in northern ireland produced no evidence of campylobacter spp. contamination and when retail packs of beef (n = 50) and pork (n = 50) were sampled these were also apparently free of campylobacter spp. however, 38% of retail packs of chicken pieces (n = 120), yielded campylobacter spp. these packs were purchased over a period of 1 year and came from a single local producer. after the species of the isolates had been determined ( ...19989674121
occurrence of thermophilic campylobacter spp. in porcine liver in northern ireland.pork liver (400) from bacon pigs (37 herds) obtained at six pork-processing in northern ireland were studied to assess the rate of contamination with campylobacter spp. these animals average 95 to 100 kg live weight. deep tissue areas were sampled immediately postevisceration and revealed that ca. 6% of livers were infected with campylobacter spp., consisting of c. coli (67%), c. jejuni (30%) and c. lari (3%). mean log10 cfu g(-1) for aerobic plate count and coliforms were 3.60 and 2.94 respecti ...19989709202
urease-positive thermophilic strains of campylobacter isolated from seagulls (larus spp.).three strains of urease-positive thermophilic campylobacter (uptc), designated a1, a2 and a3, were identified by biochemical characterization after isolation from faeces of seagulls in northern ireland in 1996. the biochemical characteristics of the strains were identical to those of strains described previously. analysis by pulsed-field gel electrophoresis (pfge) after separate digestion with apai and smai demonstrated that the respective pfge profiles were indistinguishable. the pfge analysis ...199910432626
prevalence of periodontal pathogens in localized and generalized forms of early-onset periodontitis.the primary objectives of this study were to investigate the prevalence of 8 putative periodontal pathogens in subjects with early-onset periodontitis (eop) and to evaluate the microbial differences between localized and generalized forms of this periodontal disease condition. thirty-one females and 11 males with a mean age of 30.3 (s.d. 4.0) years were examined. seventeen subjects had generalized (geop) and 25 had localized early-onset periodontitis (leop). subgingival plaque samples were assay ...200010983884
outbreak of campylobacter food-poisoning in northern ireland. 200011168160
antibiotic resistance in campylobacter spp. isolated from human faeces (1980-2000) and foods (1997-2000) in northern ireland: an update. 200111533022
demonstration of genome dna diversity of strains of urease-positive thermophilic campylobacter isolated from the natural environment by pulsed-field gel electrophoresis analysis.pulsed-field gel electrophoresis (pfge) analysis was carried out after separate digestion with apa i, sal i and sma i of the genomic dna from sixteen isolates of urease-positive thermophilic campylobacter (uptc) obtained from the natural environment, namely from oysters and mussels, in northern ireland. five nctc strains previously isolated in england were used for the analysis. although the eight isolates of uptc in northern ireland and a strain of uptc in england showed that one or no fragment ...200111549241
molecular characterisation of human campylobacteriosis in northern ireland: evidence of clonal study has compared campylobacter isolates from a human source in northern ireland over an extended period of time.200211993592
molecular diversity of campylobacter coli and c. jejuni isolated from pigs at slaughter by flaa-rflp analysis and ribotyping.a population of porcine isolates of camplobacter jejuni (n = 11) and c. coli (n = 17) were examined for genotypic relatedness employing ribotyping, as well as polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis of the flagellin (fla)a gene locus. pcr was employed to amplify a 533 bp fragment from the flaa gene, including the previously described short variable region (svr), employing the novel primers, a2 and al and successfully generated this amplicon for all ...200212449248
phenotypic diversity of campylobacter isolates from sporadic cases of human enteritis in northern ireland. 200312680629
comparison of human and porcine strains of campylobacter coli. 200312930063
fluoroquinolone resistance in environmental urease-positive campylobacters: why we don't see it. 200415056647
demonstration of the shorter flagellin (flaa) gene of urease-positive thermophilic campylobacter isolated from the natural environment in northern ireland.the pcr amplicons (about 1450 bp in length) of flaa gene fragments of 11 isolates of urease-positive thermophilic campylobacter (uptc) isolated from the natural environment not including wild birds in northern ireland were demonstrated to be shorter than those of c. jejuni 81116 and six isolates of c. jejuni and c. coli (about 1700 bp) isolated in northern ireland and japan. when the nucleotide lengths of the possible open reading frame (orf) of the flaa genes were determined, those from the 11 ...200617004648
antimicrobial resistance in campylobacter isolates from sporadic cases of acute human gastroenteritis in northern ireland. 200919568453
prevalence of campylobacter spp. in raw retail poultry on sale in northern ireland.a year-long survey of fresh, retail poultry products on sale in northern ireland was undertaken to define the prevalence of campylobacter spp. by using protocols based on iso (standard) 10272-1:2006. incubation at 37 and 42 degrees c was undertaken to increase the diversity of isolates obtained. overall, 652 isolates were identified as campylobacter spp. by using pcr and amplified fragment length polymorphic typing. phenotyping wrongly identified 21% of isolates. prevalences of campylobacter fou ...200919777882
nucleotide sequence analysis of the reca gene and discrimination of the three isolates of urease-positive thermophilic campylobacter (uptc) isolated from seagulls (larus spp.) in northern ireland.nucleotide sequencing after ta cloning of the amplicon of the almost-full length reca gene from three strains of uptc (a1, a2, and a3) isolated from seagulls in northern ireland, the phenotypical and genotypical characteristics of which have been demonstrated to be indistinguishable, clarified nucleotide differences at three nucleotide positions among the three strains. in conclusion, the nucleotide sequences of the reca gene were found to discriminate among the three strains of uptc, a1, a2, an ...200415162395
antimicrobial susceptibility of campylobacter spp. isolated from broiler chickens in northern ireland.between february 2000 and october 2001, cloacal swabs were collected from 387 broiler chicken flocks in northern ireland. campylobacter isolates from the 262 positive flocks were tested with common antimicrobial agents using a disc diffusion method and by etests. resistance to erythromycin, gentamicin and chloramphenicol was <1%, whereas for ampicillin, nalidixic acid and tetracycline, resistance was 33%, 10% and 13%, respectively. ciprofloxacin resistance was 3%, one of the lowest in recent rep ...200312865393
salmonella and campylobacter contamination of raw retail chickens from different producers: a six year survey.between 1995 and 2000, a prospective survey was undertaken to investigate the levels of contamination of raw retail chickens (n = 1,127) with salmonella and campylobacter. the levels of contamination over the 6-year period were 11 % (95 % ci +/- 6.5%) for salmonella, and 57% (95% ci +/- 95%) for campylobacter. s. bredeney (20%) and s. enteritidis (18%) were the dominant serovars. although salmonella contamination was higher than in an earlier survey we conducted (7%), since 1998 it has declined ...200212558349
occurrence of campylobacter spp. in water in northern ireland: implications for public health.the occurrence of campylobacter spp was examined in a variety of waters in northern ireland. conventional cultural techniques were employed with 768 water specimens, including drinking waters (tap, spring, bore hole and bottled) and recreational waters (swimming pool, lough, river and sea). positive waters included 1/11 (9.1%) drinking water from untreated well water, as well as 5/12 (41.7%) untreated surface waters from loughs and 7/8 (87.5%) untreated river waters. overall, untreated surface w ...200111795758
low incidence of campylobacter enteritis in northern a prospective survey carried out over 9 months in 1984 in the department of bacteriology, belfast city hospital, campylobacter jejuni was isolated from 24 out of 1200 faecal specimens (2%) from patients with acute diarrhoea. this isolation rate is much lower than that from other parts of mainland britain which report an isolation rate of between 8 and 15%. it is difficult to explain this large discrepancy but the limited availability of unpasteurized milk and the generally worse summer here ( ...19863794324
phylogenetic analysis of urease-positive thermophilic campylobacter (uptc) strains based on the molecular characterization of the flaa gene.molecular cloning, nucleotide sequencing, and characterization of the flaa gene from additional isolates of urease-positive thermophilic campylobacter (uptc) were performed. these isolates were obtained from the natural environment in northern ireland (nā€‰=ā€‰9 from mussels) and in england (nā€‰=ā€‰1 from sea water). all isolates carried the shorter flaa gene, [open reading frames (orfs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaa pseudogenes. the up ...201121874596
phenotypic diversity of campylobacter isolates from sporadic cases of acute human gastroenteritis in northern ireland. 201020373679
campylobacter spp. in conventional broiler flocks in northern ireland: epidemiology and risk factors.risk factors for campylobacter infection in conventional broiler flocks in the time period up to the first removal of birds to slaughter were investigated over a maximum of five consecutive production cycles in a cohort of 88 broiler farms in northern ireland. samples for campylobacter culture, which consisted of 14 cloacal swabs per flock, were collected from one house on each farm prior to the first depopulation of birds. in total 388 flocks were sampled, of which 163 tested positive for campy ...200818249451
salmonella and campylobacter in united kingdom retail raw chicken in 2005.the united kingdom food standards agency commissioned a survey of salmonella and campylobacter in raw, whole chickens at retail in wales and northern ireland between march and december 2005 to measure the baseline prevalence rates of these two significant pathogens. in total, 877 retail samples were examined for campylobacter and salmonella by enrichment methods. overall contamination rates of 70.2% for campylobacter and 4.0% for salmonella were found. there was a statistically significant diffe ...200717803153
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