| complete genome sequencing of dengue virus type 1 isolated in buenos aires, argentina. | dengue (den) constitutes a major viral arthropod-borne human illness. south america was last considered free of dengue two decades ago when a dramatic increase in the number of dengue fever and hemorrhagic dengue cases had been reported. five viruses were isolated in buenos aires city from the 1999-2000 paraguay outbreak. rt-pcrs obtained directly from plasma were cloned into pgemt vectors and sequences of the structural genes and ns1 were analyzed. three viruses were full-length sequenced from ... | 2004 | 15041181 |