| detection of rotaviruses in the day care environment by reverse transcriptase polymerase chain reaction. | group a rotavirus is an important cause of morbidity among infants and toddlers in day care centers. transmission by the fecal-oral route is well established, but fomites and environmental surfaces may also play an important role in transmission. a highly sensitive polymerase chain reaction (pcr) assay was used to detect rotavirus rna in day care environments. areas sampled included floors, diaper change areas, toy balls, and other surfaces. in two centers undergoing outbreaks of rotavirus, 7 (3 ... | 1992 | 1380049 |
| analysis of serotypes and electropherotypes of equine rotaviruses isolated in the united states. | equine group a rotaviruses isolated over a 10-year period in new york state, new jersey, kentucky, and texas were compared serotypically and electropherotypically. all isolates were determined to be serotype 3 by reaction with hyperimmune antiserum to the serotype 3 h-2 strain of equine rotavirus. all displayed rna electrophoretic migration patterns related to that of the h-2 strain but distinct from that of serotype 5 strain h-1. a serologic survey of 184 mares in kentucky, which was done to de ... | 1991 | 1647407 |
| serotype variation of human group a rotaviruses in two regions of the usa. | the first longitudinal study of group a rotavirus serotype distribution in the usa is reported. elisas incorporating neutralizing monoclonal antibodies specific for the vp7 protein of serotypes 1, 2, 3, and 4 were used to determine the antigenic variation of group a rotaviruses in two collections of stool specimens. stool samples were collected from children hospitalized during 1979-1989 at texas children's hospital, houston, and from children from a more rural population hospitalized during 198 ... | 1990 | 2167332 |
| molecular epidemiology of rotavirus in children attending day care centers in houston. | serotype distribution of group a rotavirus in children attending day care centers has not been previously reported. fifteen centers in houston participated in longitudinal monitoring of rotavirus infection during one to three consecutive rotavirus seasons. monitoring included daily recording of the presence of diarrhea and weekly stool collection. stool specimens containing rotavirus were analyzed by a monoclonal antibody (mab)-based elisa specific for the vp7 protein of serotypes 1, 2, 3, and 4 ... | 1990 | 2169496 |