| multiplex rt-pcr for rapid detection of viruses commonly causing diarrhea in pediatric patients. | multiplex rt-pcr method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus a, b, and c, adenovirus, astrovirus, norovirus gi and gii, sapovirus, aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and saffold virus. the sensitivity of the method was evaluated and tested with 751 fecal specimens collected from japanese children with acute diarrhea. several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. mi ... | 2017 | 27735999 |
| a single-tube multiplex pcr for rapid detection in feces of 10 viruses causing diarrhea. | a novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. the assay was targeted to detect group a and c rotaviruses, adenovirus, norovirus gi, norovirus gii, sapovirus, astrovirus, aichi virus, parechovirus, and enterovirus. a total of 235 stool samples were collected from infants and children with acute gastroenteritis in kyoto, japan, from 2008 to 2009, then tested by this novel multiplex pcr and compared with a multiplex pcr described previously ... | 2011 | 21349292 |
| molecular epidemiology of enteric viruses in patients with acute gastroenteritis in aichi prefecture, japan, 2008/09-2013/14. | acute gastroenteritis is a critical infectious disease that affects infants and young children throughout the world, including japan. this retrospective study was conducted from september 2008 to august 2014 (six seasons: 2008/09-2013/14) to investigate the incidence of enteric viruses responsible for 1,871 cases of acute gastroenteritis in aichi prefecture, japan. of the 1,871 cases, 1,100 enteric viruses were detected in 978 samples, of which strains from norovirus (nov) genogroup ii (60.9%) w ... | 2016 | 26647761 |