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detection and genetic characterization of human enteric viruses in oyster-associated gastroenteritis outbreaks between 2001 and 2012 in osaka city, japan.enteric viruses are an important cause of viral food-borne disease. shellfish, especially oysters, are well recognized as a source of food-borne diseases, and oyster-associated gastroenteritis outbreaks have on occasion become international occurrences. in this study, 286 fecal specimens from 88 oyster-associated gastroenteritis outbreaks were examined for the presence of 10 human enteric viruses using antigenic or genetic detection methods in order to determine the prevalence of these infection ...201424415518
detection and characterization of a human g9p[4] rotavirus strain in japan.in a surveillance system in osaka city, japan, 48 sporadic rotavirus a (rva) infections were detected during 2008/2009-2011/2012 seasons. the g/p-genotypes of detected rvas were g1p[8], g2p[4], g3p[8], g9p[4], and g9p[8]. although g9p[4] is a rare genotype that had not been reported in japan, it was the second most prevalent genotype, following g1p[8], and accounted for 35.3% of rva cases in the 2011/2012 season. further genotyping revealed that the g9p[4] strain had genotype 2 internal protein ...201525907907
genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in japan in 2004-2005.a total of 752 fecal specimens collected from july 2004 to june 2005 from children with acute gastroenteritis in four localities in japan (maizuru, tokyo, sapporo, and osaka) were screened for group a rotavirus by rt-pcr. it was found that 82 (10.9%) specimens were positive for group a rotavirus. the g-(vp7 genotypes) and p-(vp4 genotypes) types were further investigated. the p-types of 18 rotavirus strains, which could not be typed by rt-pcr, were determined by sequencing analysis. of these, 94 ...200717110174
outbreak of sapovirus infection among infants and children with acute gastroenteritis in osaka city, japan during 2004-2005.one hundred and twenty five fecal specimens were collected from sporadic cases of acute gastroenteritis in a pediatric clinic in osaka city, japan from july 2004 to june 2005 and tested for the presence of rotavirus, norovirus, sapovirus, astrovirus, and adenovirus by rt-multiplex pcr. among diarrheal viruses detected, norovirus was the most prevalent (19.2%, 24 of 125), followed by group a rotavirus (18.4%, 23 of 125), astrovirus (1.6%, 2 of 125), and adenovirus (0.8%, 1 of 125), respectively. ...200616628578
viral diarrhea in japanese children: results from a one-year epidemiologic study.a total of 557 fecal specimens from infants and children with acute gastroenteritis in five places (maizuru, tokyo, sapporo, saga and osaka) in japan from july 2002 to june 2003 were tested for the presence of diarrheal viruses by rt-pcr, prha, rna-page and latex agglutination methods. of these, 56.4% (314) were found positive for diarrheal viruses. among them, group a rotavirus was the most prevalent (43.6%, 137 of 314) followed by norovirus (29.9%, 94 of 314), adenovirus (7.6%, 24 of 314), gro ...200515819174
etiologic agents of acute gastroenteritis among japanese infants and children: virus diversity and genetic analysis of sapovirus.a total of 371 fecal specimens from infants and children with acute gastroenteritis in maizuru, tokyo and osaka, japan from july 2002 to june 2003 were tested for the presence of diarrheal viruses by reverse transcription-polymerase chain reaction (rt-pcr), reverse passive hemagglutination (prha), rna-polyacrylamide gel electrophoresis (page), latex agglutination and sequence analysis methods. among diarrheal viruses detected, group a rotavirus was the most prevalent (42.2%) followed by noroviru ...200515789264
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