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frequency of escherichia coli o157:h7 isolation from stool specimens.during a 6-month period, 7252 faeces specimens were examined for escherichia coli serotype o157:h7. forty-nine specimens (0.7%) from 19 patients yielded this organism. escherichia coli o157:h7 was the third most frequently isolated bacterial pathogen, following campylobacter jejuni and (or) salmonella sp. although regional variation between laboratories determined whether campylobacter jejuni or salmonella was the primary bacterial pathogen isolated, e. coli o157:h7 was consistently isolated mor ...19873319115
evaluation of a one-step biochemical screening test to determine pathogenic strains of yersinia enterocolitica. 200312756812
enteric illness in ontario, canada, from 1997 to 2001.enteric illness is a common problem worldwide. in ontario (population of 11.4 million, 2001 census of canada), laboratory-confirmed cases of "reportable" enteric diseases are reported to local health units. public health staff members investigate these illnesses and subsequently report details to the ministry of health and long-term care through an electronic reporting system. from 1997 to 2001, 44,451 sporadic cases of illness attributable to eight enteric pathogens (campylobacter, salmonella, ...200312800994
descriptive epidemiology of enteric illness for selected reportable diseases in ontario, 2003. 200617175595
occurrence of salmonella, campylobacter, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes in swine.the objective of this study was to investigate the occurrence of major bacterial foodborne pathogens in swine. in total, 359 samples from manure storage tanks (91) and fresh pooled faeces (268) obtained from finisher (110), sows (78) and weanlings (80) were collected and tested. campylobacter, salmonella, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes were isolated from 36.5%, 31.5%, 5.8%, 3.3% and 3.3% of samples respectively. all e. coli o157 isolates found on 10 far ...201019638163
tularemia, plague, yersiniosis, and tyzzer's disease in wild rodents and lagomorphs in canada: a review.information related to infection of wild rodents or lagomorphs in canada by francisella tularensis, yersinia pestis, other yersinia spp., and clostridium piliforme was searched for this study. reports on tularemia in humans linked to these species came from diagnostic databases, literature, wildlife health specialists, and public health agencies. tularemia has been diagnosed in 8 species of wild rodent and 2 species in the genus lepus in canada. tularemia occurred in wild animals, or in humans a ...200920190973
an evaluation of methods for the isolation of yersinia enterocolitica from surface waters in the grand river watershed.yersinia enterocolitica is a foodborne pathogen, but the importance of water as a route of exposure for human infection is not well known. y. enterocolitica isolation methods were developed primarily for food and clinical samples, and may not be effective for use with environmental samples. the objective of this study was to assess the recovery of y. enterocolitica from surface water used for drinking water treatment. four enrichment broths and an alkaline treatment protocol were compared for th ...200919491491
simulating the effect of eliminating routine bacterial screening on the negative predictive value of the ontario fish hatchery disease monitoring program.we determined the impact of eliminating routine screening for aeromonas salmonicida and yersinia ruckeri on the efficacy of the ontario ministry of natural resources (omnr) fish disease monitoring program, using monte carlo simulation. because the main purpose of the program is to prevent transferring infected fish among omnr hatcheries, or to wild fish populations through stocking waterways, the hatchery-level negative predictive value (hnpv) was used as an indicator of monitoring efficacy. the ...200111448498
host factors associated with the detection of aeromonas salmonicida and yersinia ruckeri in ontario, canada government fish hatcheries.this paper presents an epidemiological investigation of ontario ministry of natural resources fish health laboratory data from 1981 to 1997, to determine whether fish species and age were associated with lot-level detection of aeromonas salmonicida and yersinia ruckeri in hatchery fish. in stepwise logistic regression, the species brook trout and back-cross (lake trout crossed with the hybrid "splake") were more likely to test a. salmonicida-positive compared to all other species reared in the h ...200111311951
outbreaks of yersiniosis caused by yersinia pseudotuberculosis in farmed cervids.yersiniosis, caused by yersinia pseudotuberculosis, was diagnosed in 25 deer submitted to the veterinary diagnostic laboratories of the ontario ministry of agriculture and food from 8 farms in ontario, canada, over the 4-year period of january 1990 to december 1993. the organism was cultured, usually in large numbers, from the intestines and, less frequently, mesenteric lymph nodes and/or the spleen of 13 red deer (cervus elaphus), 6 elk (c. e. canadiensis), 4 fallow deer (dama dama), and 2 red ...19957779969
yersinia enterocolitica isolated from throats of swine in eastern and western canada.examination of the throat flora from swine in ontario for yersinia enterocolitica found the incidence of serotype o:3 to vary from 20% from tonsils to 50% for throat swabs and 55% for tongues. in contrast, there were no isolations of serotype o:3 from throat swabs taken from swine in the western provinces of british columbia and alberta. this incidence of serotype o:3 in swine correlates well with the human incidence of the same serotype which is 81% for all human isolations of y. enterocolitica ...19817332882
yersinia enterocolitica and yersinia pseudotuberculosis from wildlife in ontario. 19744612176
survey on the incidence of yersinia enterocolitica in the province of ontario. 19734586389
survey on the incidence of yersinia enterocolitica infection in canada.data pertaining to 278 cultures of yersinia enterocolitica isolated in canada are summarized in this paper. of this amount, 256 were isolated from humans, whereas 22 were of nonhuman sources. the typing of these cultures is presented together with their geographical location. y. enterocolitica serotype o:3 biotype 4, phage type 9b, was practically the only serotype present in the province of quebec. this serotype o:3 was also predominant in ontario, followed by serotypes o:5,27 and o:6,30; other ...19744472776
isolation of yersinia enterocolitica from surface and well waters in ontario.yersinia enterocolitica was isolated from forty-three surface- and well-water samples and one municipal supply. in many cases these samples were negative for coliform bacteria. after incubation of membrane filters on m-endo agar les at 35 degrees c for total coliform analysis, filters were held an additional 24 h at room temperature. this method was more productive for isolation of waterborne types of y. enterocolitica than was cold enrichment in buffer alone or in combination with modified rapp ...1978709433
canadian experience with yersinia enterocolitica (1966--1977).data pertaining to 1,219 cultures of yersinia enterocolitica isolated in canada are summarized. of the 977 cultures isolated from humans, y. enterocolitica serotype 0:3, biotype 4, phage type ixb was the most predominant type in ontario, quebec and the four eastern provinces. in the western provinces the predominant strains were indol-positive, serotypes 0:8; 5,27 and 4,32. most of the 242 cultures of nonhuman origin, except those isolated from swine and a few isolated from wild animals, were in ...1979535372
association of yersinia enterocolitica with the manufacture of cheese and occurrence in pasteurized milk.raw milk in southern ontario frequently contains yersinia enterocolitica. the potential for transmission of this organism by cheese manufactured from unpasteurized milk was evaluated by examination of milk and cheese curd samples from cheese manufacturing plants and finished cheddar and italian cheeses. the incidence of y. enterocolitica was lower in cheese curd samples (9.2%) than in raw milk (18.2%). most of the curd samples showed a positive phosphatase test, indicating production from raw mi ...1978211937
microbiological identification and analysis of swine tonsils collected from carcasses at slaughter.the primary objective of this 7-month study was to determine the prevalence of porcine pathogens of the tonsil of the soft palate of swine at slaughter. additional objectives were to determine if sampling the carcasses of normal or abnormal hogs provided different microbiological profiles and if the slaughter plant provides a feasible sampling frame and environment for detecting and monitoring important pathogens in tonsils that have health implications for both swine and humans. a total of 395 ...201121731180
the detection of yersinia enterocolitica in surface water by quantitative pcr amplification of the ail and yada genes.yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. pcr-based methods have been used to detect y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. in this study, quantitative pcr (qpcr)-based methods targeting the yersinia virulence genes ail and yada were used to survey the grand river watershed in southern ontario, canada. initial testing of reference strains showed that ail a ...201020375478
prevalence of yersinia enterocolitica shedding and bioserotype distribution in ontario finisher pig herds in 2001, 2002, and 2004.we investigated characteristics of yersinia enterocolitica infection in ontario finisher pig herds. our specific objectives were to estimate or test: prevalence of y. enterocolitica shedding in finisher pigs, bioserotype distribution, agreement between the herd-level tests based on sampling pig and pooled fecal samples, whether bioserotypes cluster by farms, and whether y. enterocolitica-positive herds cluster spatially. in total, 3747 fecal samples were collected from 100 farms over the years 2 ...201019926152
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