| evaluation and validation of a monoclonal immunofluorescent reagent for direct detection of bordetella pertussis. | an outbreak of pertussis in manitoba, canada, provided an opportunity to evaluate the recently developed monoclonal antibody (mab) bl-5 for the direct detection of bordetella pertussis. the mab recognizes a lipooligosaccharide epitope. a total of 1,507 consecutive nasopharyngeal swabs for culture and companion smears for direct fluorescent-antibody (dfa) detection were evaluated at cadham provincial laboratory between september and november 1994. the cutoff for dfa positivity was four fluorescin ... | 1995 | 8576336 |