| selective adherence of non-typeable haemophilus influenzae (nthi) to mucus or epithelial cells in the chinchilla eustachian tube and middle ear. | frozen sections of chinchilla eustachian tube (et) and middle ear mucosa were incubated with either fitc-labeled non-typeable haemophilus influenzae (nthi) or bordetella pertussis. the number of bacteria adherent to "roof" vs "floor" regions was compared for each of three anatomic portions of the et and for middle ear epithelium noting whether bacteria adhered to mucus or to epithelial cells. nthi strains adhered significantly greater to mucus in the et lumen whereas b. pertussis preferentially ... | 1996 | 8938642 |
| kinetics of the ascension of nthi from the nasopharynx to the middle ear coincident with adenovirus-induced compromise in the chinchilla. | to determine the kinetics of ascension of the eustachian tube (et) by non-typeable haemophilus influenzae (nthi) in situ from the nasopharynx to the middle ear using an experimental model of otitis media (om), we examined snap-frozen sections of chinchilla et and middle ear mucosa for adherent bacteria over a 14 day time period. via fluorescent- and transmission electron-microscopy, we found that nthi preferentially adhered not to the epithelial cells but to the mucus in the et and gradually asc ... | 1997 | 9245624 |
| mouse models for the study of mucosal vaccination against otitis media. | otitis media (om) is one of the most common infectious diseases in humans. the pathogenesis of om involves nasopharyngeal (np) colonization and retrograde ascension of the pathogen up the eustachian tube into the middle ear (me). due to increasing rates of antibiotic resistance, there is an urgent need for vaccines to prevent infections caused by the most common causes of bacterial om, including nontypeable haemophilus influenzae, streptococcus pneumoniae and moraxella catarrhalis. current vacci ... | 2008 | 18295938 |