Publications

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simplified pcr for detection of haemophilus ducreyi and diagnosis of chancroid.a simplified pcr was developed for detection of haemophilus ducreyi in samples from chancroid patients. the strategy included a straightforward chloroform extraction sample preparation method, a one-tube nested pcr to minimize contamination risks, and a colorimetric method for detection of products. primers were designed from published nucleotide sequences of the 16s rrna gene of h. ducreyi, with longer outer primers for annealing at a higher temperature and shorter inner primers labelled with b ...19957540625
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