simplified pcr for detection of haemophilus ducreyi and diagnosis of chancroid. | a simplified pcr was developed for detection of haemophilus ducreyi in samples from chancroid patients. the strategy included a straightforward chloroform extraction sample preparation method, a one-tube nested pcr to minimize contamination risks, and a colorimetric method for detection of products. primers were designed from published nucleotide sequences of the 16s rrna gene of h. ducreyi, with longer outer primers for annealing at a higher temperature and shorter inner primers labelled with b ... | 1995 | 7540625 |