no detectable xmrv in subjects with chronic fatigue syndrome from quebec.we investigated the presence of xmrv in a cohort of quebec patients with chronic fatigue syndrome (cfs). dna was purified from activated peripheral blood mononuclear cells (pbmcs) and pcr was used to detect xmrv gag and env in 72 patients. anti-xmrv antibodies were searched in sera of 62 patients by western blot analysis. attempts to detect xmrv antigens was made, using immunofluorescence with gag anti-p30 antiserum on activated pbmc from 50 patients. plasma viremia was measured by rt-pcr on 9 s ...201121925693
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