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hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to ehrlichiae and detection of dna of agent of human granulocytic ehrlichiosis by pcr.ixodid ticks were collected from connecticut, massachusetts, missouri, pennsylvania, rhode island, and british columbia (canada) during 1991 to 1994 to determine the prevalence of infection with hemocytic (blood cell), rickettsia-like organisms. hemolymph obtained from these ticks was analyzed by direct and indirect fluorescent antibody (fa) staining methods with dog, horse, or human sera containing antibodies to ehrlichia canis, ehrlichia equi, or rickettsia rickettsii. of the 693 nymphal and a ...19958567911
detection of babesia and anaplasma species in rabbits from texas and georgia, usa.rabbits have been shown to harbor a suite of zoonotic organisms, including a babesia species, borrelia burgdorferi, and anaplasma phagocytophilum. in this study, we conducted a molecular survey for various tick-borne pathogens in three species of rabbits from texas and georgia. of 18 black-tailed jackrabbits (lepus californicus) tested from texas, six (28%) were polymerase chain reaction (pcr) positive for babesia, and nucleotide sequencing revealed two distinct species or strains. two jackrabbi ...200616584322
the pcs20 pcr assay for ehrlichia ruminantium does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the united states of america.white-tailed deer are susceptible to heartwater (ehrlichia [cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the united states. it is vital, therefore, to validate a highly specific and sensitive detection method for e. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as ehrlichia chaffeensis, anaplasma (ehrlichia) phagocytophilum (hge agent) and ...200415373331
evaluation of granulocytic ehrlichiosis in dogs of missouri, including serologic status to ehrlichia canis, ehrlichia equi and borrelia burgdorferi.canine granulocytic ehrlichiosis was diagnosed in 37 dogs by finding ehrlichial morulae in 0.1 to 26.2% of their blood neutrophils and eosinophils. all 37 dogs had clinical signs of arthritis or muscular stiffness. titer to ehrlichia canis was determined in sera from 31 of the 37 dogs; 25 dogs had titer ranging from 1:20 to 1:5,120. in the other 6 dogs, titer to e canis was less than 1:10. the most common hematologic abnormality in these dogs, other than rickettsiemia, was thrombocytopenia. gran ...19921539918
predominance of ehrlichia ewingii in missouri dogs.to investigate the species distribution of ehrlichia present in missouri dogs, we tested 78 dogs suspected of having acute ehrlichiosis and 10 healthy dogs. blood from each dog was screened with a broad-range 16s rrna gene pcr assay that detects known pathogenic species of ehrlichia and anaplasma: the species was determined by using species-specific pcr assays and nucleotide sequencing. ehrlichia antibody testing was performed by using an indirect immunofluorescence assay with ehrlichia chaffeen ...200314532192
detection of ehrlichia spp. in the blood of wild white-tailed deer in missouri by pcr assay and serologic analysis.blood samples collected from wild deer in missouri in november of 2000 and 2001 were positive by pcr assays for ehrlichia chaffeensis (50 of 217; 23%), ehrlichia ewingii (44 of 217; 20%), and anaplasma species (214 of 217; 99%). nucleotide sequences of selected amplicons from the assay for anaplasma matched sequences of the white-tailed deer agent. serologic analysis of 112 deer sampled in 2000 showed a very high prevalence of antibodies to e. chaffeensis (97 of 112; 87%) and a low prevalence of ...200312624063
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