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surveillance of chronic wasting disease in sika deer, cervus nippon, from tokachi district in hokkaido.surveillance of chronic wasting disease (cwd) was conducted by performing western blot analysis of tissue samples from 136 sika deer (cervus nippon) killed by hunters in the tokachi district of hokkaido island. no prion protein (prpsc) associated with cwd was detected in any of the samples. to assess amino acid polymorphisms of the sika deer prp gene, nucleotide sequencing of the prp gene was performed. the only amino acid polymorphisms detected were 3 silent mutations at nucleotide positions 63 ...200515805745
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