[isolation and identification of borrelia burgdorferi sensu lato from ticks in six provinces in china.]objective: to understand the carrying status of borrelia burgdorferi in ticks from the mountain areas from six representative provinces, including jilin, shanxi, gansu, qinghai, guizhou and hunan in china. methods: flagging and trapping methods were used to collect ticks in forest area and culture medium was used to isolate the pathogen. nested-pcr was used to detect the germ-carrying rate of ticks. results: more than 2200 ticks from six representative provinces were collected and 1000 ticks wer ...201021223661
distribution of borrelia burgdorferi sensu lato in china.we genotyped 102 borrelia burgdorferi sensu lato strains isolated from ticks, animals, and patients in 11 provinces in china by pcr-restriction fragment length polymorphism (pcr-rflp) amplification of 5s (rrf)-23s (rrl) rrna gene spacer amplicons and multilocus sequence analysis (mlsa). the results showed that borrelia garinii was the main genotype in china (65/102) and that it was distributed mainly in northern china. borrelia afzelii was the second most frequently found species (22/102), and i ...201021106783
molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ...201626943995
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