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bacterial degradation of n,n-diethyl-m-toluamide (deet): cloning and heterologous expression of deet hydrolase.pseudomonas putida dtb grew aerobically with n,n-diethyl-m-toluamide (deet) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. the former was further metabolized via 3-methylcatechol and meta ring cleavage. a gene from dtb, dtha, was heterologously expressed and shown to encode the ability to hydrolyze deet into 3-methylbenzoate and diethylamine.200717337538
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