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a new extant respirometric assay to estimate intrinsic growth parameters applied to study plasmid metabolic burden.start-up phenomena in microbial biokinetic assays are not captured by the most commonly used growth-related equations. in this study we propose a new respirometric experimental design to estimate intrinsic growth parameters that allow us to avoid these limitations without data omission, separate mathematical treatment, or wake-up pulses prior to the analysis. identifiability and sensitivity analysis were performed to confirm the robustness of the new approach for obtaining unique and accurate es ...201019718700
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