| molecular identification of ehrlichia species and 'candidatus neoehrlichia mikurensis' from ticks and wild rodents in shizuoka and nagano prefectures, japan. | a total of 293 ticks and 111 wild rodents that were collected in shizuoka and nagano prefectures, japan, were examined for infection of ehrlichia species and 'candidatus neoehrlichia mikurensis.' the 16s rdna or the omp-1 gene of these bacterial dnas were detected from the spleens of tick-inoculated mice (5 positive/total 29 mice) or from the spleens of wild rodents (25 positive/total 111 rodents) by pcr amplifi-cation. sequencing of the 16s rdna revealed ehrlichia spp. from the 5 tick-inoculate ... | 2006 | 16428872 |
| characterization of ehrlichia species from ixodes ovatus ticks at the foot of mt. fuji, japan. | a total of 390 adult ticks (288 ixodes ovatus and 102 i. persulcatus ) collected at the foot of mt. fuji and two near cities in shizuoka prefecture, japan, were examined for ehrlichia infection by isolation with laboratory mice from whole tick tissues. ehrlichial dnas were detected from the spleens of mice inoculated with tissues from i. ovatus, but not i. persulcatus. the prevalence of ehrlichiae in the ticks was estimated to be ca. 3%. the 16s rdna analysis revealed that the sequences of 8 ehr ... | 2004 | 15502406 |
| detection of ehrlichial dna in small rodents captured in a woodland area of hokkaido, the northernmost island of japan, where lyme disease is endemic. | the ehrlichial gene was detected in small rodents trapped in a lyme disease-endemic area in hokkaido, the northernmost island of japan. primer pairs of 16s rdna targeting the genus ehrlichia and other regions of the 16s rdna specific for e. chaffeensis and e. muris were used for identification. the dna fragment specific for 16s rdna of ehrlichia spp. was detected in 4 of 94 apodemus speciosus mice (positive rate: 4.3%) and 5 of 73 clethrionomys rufocanus bedfordiae mice (positive rate: 6.8%). th ... | 2005 | 16249629 |