| development of a simple and effective method for specific detection of pepper mild mottle virus. | a digoxigenin (dig)-labeled cdna probe complementary to the region from 5,256-6,300 nt of pepper mild mottle virus (pmmov) genome was synthesized. the specificity and sensitivity of the probe was tested by the dot-blot hybridization. the detection limit of this method was equivalent to 0.8 microg of fresh infected tissue in each spot. double-antibody sandwich (das) elisa and rt-pcr had the detection limit 39 microg and 0.008 microg of fresh infected tissue, respectively. we evaluated leaf, fruit ... | 2009 | 19301947 |