| coupling 16s-its rdna clone libraries and automated ribosomal intergenic spacer analysis to show marine microbial diversity: development and application to a time series. | we outline an approach to simultaneously assess multilevel microbial diversity patterns utilizing 16s-its rdna clone libraries coupled with automated ribosomal intergenic spacer analysis (arisa). sequence data from 512 clones allowed estimation of arisa fragment lengths associated with bacteria in a coastal marine environment. we matched 92% of arisa peaks (each comprising >1% total amplified product) with corresponding lengths from clone libraries. these peaks with putative identification accou ... | 2005 | 16104869 |