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minimizing elisa background in the diagnosis of swine trichinellosis.after confirming that long-term serum storage (frozen at -20 c for greater than 3 mo) causes optical density to drift upward, several modifications of an enzyme-linked immunosorbent assay (elisa) protocol were evaluated to identify a protocol that would reduce background in porcine sera tested for trichinellosis. modifications evaluated included blocking the antigen-coated elisa plate with sample diluent containing 10% bovine serum albumin (bsa) or 10% nonfat milk powder (bovine lacto transfer o ...19921403424
trichinella spiralis in florida wildlife. 19715133888
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