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evaluation of pcr for diagnosis of american cutaneous leishmaniasis in an area of endemicity in northeastern brazil.pcr-based approaches targeting kinetoplast dna were evaluated for the diagnosis of american cutaneous leishmaniasis (acl) in regions of endemicity in northeastern brazil. a total of 119 cutaneous biopsy specimens from patients with acl and nonleishmaniasis cutaneous lesions were studied. two pcr-based systems were used; one was specific for the subgenus viannia, and the other was specific for the genus leishmania. the pcr specific for the subgenus viannia had a sensitivity of 95.4%, whereas the ...200212354848
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