| isolation and identification of legionella and their host amoebae from weak alkaline carbonate spring water using a culture method combined with pcr. | legionella were detected with the direct dna extraction method, legionella culture method, and free-living amoebae (fla) culture method from weak alkaline carbonate spring water in taiwan. moreover, we also investigated the existence of acanthamoeba, hartmannella, and naegleria, ubiquitous fla in aquatic environments, to identify the correlations between existing legionella. this study reports detecting legionella in 15 of the 51 weak alkaline carbonate spring water samples (29.4%). this work al ... | 2011 | 21537990 |