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primary isolation of spotted fever group rickettsiae from amblyomma cooperi collected from hydrochaeris hydrochaeris in brazil.this paper reports the first isolation of a spotted fever group rickettsia from an amblyomma cooperi ixodid collected from a capybara (hydrochaeris hydrochaeris) in an endemic area of spotted fever in the county of pedreira, state of são paulo, brazil. isolation was performed in vero cell culture and submitted to immunofluorescence, using antibody from rickettsia rickettsii-positive human serum.19969040845
epidemiological aspects of the brazilian spotted fever: seasonal activity of ticks collected in an endemic area in são paulo, brazil.ticks were collected from vegetation and animals at monthly intervals during one year (1993-1994) in an endemic area of brazilian spotted fever in the country of pedreira, state of são paulo. six species of ticks were identified amblyomma cajennense, amblyomma cooperi, amblyomma triste, anocentor nitens, rhipicephalus sanguineus and boophilus microplus. only the first species was sufficiently numerous to permit a quantitative study with seasonal activity, although the distribution and source of ...19979197151
diversity of ectoparasites in sheep flocks in são paulo, brazil.the occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. sheep production used to be a predominantly family activity in the state of são paulo (brazil), but it began to become a commercial activity in the past decade. thus, information about the ectoparasites existing in sheep flocks has become necessary. the present data were obtained by means of questionnaires sent to all sheep breeders belonging to the 'associação paulista de criadores de ovinos' (aspaco; ...200011020945
parasitism by ixodes didelphidis and i. loricatus (acari: ixodidae) on small wild mammals from an atlatic forest in the state of sao paulo, brazil.from january 1995 to june 1996, ticks were studied in a fragment of atlantic forest in a residential area in the city of itapevi, state of sao paulo, brazil. cases of human lyme disease-like illness were registered in this area during the spring of 1992. the monthly relative density of ticks was determined and the influence of seasonal climatic conditions was evaluated as well as the relationship between ticks and hosts. ticks (n = 88) were collected from small mammals captured (n = 134) in mont ...200011185614
risk factors to tick infestations and their occurrence on horses in the state of são paulo, brazil.from december 1998 to march 1999, 40 stud farms were studied in the state of são paulo, brazil. during visits to farms, horses reared under grazing conditions were examined for the presence of ticks. on each farm visit, horse pastures were closely inspected and a questionnaire was given to the farm supervisor with the purpose of gaining information about ecological and management variables (independent variables) that could be associated with the presence and infestation levels of ticks on the f ...200111337122
seasonal dynamics of ticks (acari: ixodidae) on horses in the state of são paulo, brazil.natural tick infestations were assessed every 14 days on horses over a 2-year period. amblyomma cajennense adult ticks were counted individually, without detachment from the horses. larvae and nymphs of a. cajennense were collected using a rubber scraper that scratched engorged immature ticks from the host. adult females of anocentor nitens larger than 4mm length were counted on the horses. blood samples were also obtained from the horses every 14 days and macroclimatic data were obtained for th ...200211879967
gynandromorphism in amblyomma cajennense and rhipicephalus sanguineus (acari: ixodidae).during a survey of ticks on horses in november 2000, at the university of são paulo farm in pirassununga county, são paulo, brazil, 1 gynandromorph of amblyomma cajennense was collected from a naturally infested horse. in another survey on dogs in march 2001, in the urban area of monte negro, rondônia, brazil, a gynandromorph of rhipicephalus sanguineus was collected from a naturally infested dog. both specimens are described and classified as perfect bipartite protogynanders.200212197141
ticks (acari: ixodidae) associated with domestic dogs in franca region, são paulo, brazil.ticks on 140 domestic dogs from both urban and rural areas of franca region in são paulo state were identified with 102 dogs from urban areas and 38 from rural areas. of urban dogs, 27.5% were infested exclusively by rhipicephalus sanguineus ticks. of the rural dogs, 36.8% were infested with the following tick species: r. sanguineus, boophilus microplus, amblyomma ovale and a. cajennense. mixed infestations included a dog hosting a. cajennense and a. ovale and another with b. microplus and r. sa ...200112455880
ticks (acari: ixodidae) on wild marsh-deer (blastocerus dichotomus) from southeast brazil: infestations before and after habitat loss.the lake from porto-primavera hydroelectric power station inundated an area of 2,200 km2 at the border of são paulo and mato-grosso do sul states, brazil. infestations by ticks were evaluated on 135 marsh deer, blastocerus dichotomus (illiger), captured before and after inundation. ticks were collected for identification, and infestation level of animals was assessed by scoring. deer were divided into four groups according to capture location and temporal relation to the inundation. groups 1, 2, ...200312943103
rickettsia species infecting amblyomma cooperi ticks from an area in the state of são paulo, brazil, where brazilian spotted fever is endemic.owing to the potential role of the tick amblyomma cooperi in the enzootic cycle of rickettsia rickettsii, the etiologic agent of brazilian spotted fever (bsf), this study evaluated infection by rickettsia species in a. cooperi ticks collected from an area in brazil where bsf is endemic. among a total of 40 a. cooperi adult ticks collected in an area of bsf endemicity in the state of são paulo, pcr analysis detected dna of rickettsia bellii in 16 ticks (40%), and 3 other ticks (7.5%) were positiv ...200414715737
study of the seasonal dynamics, life cycle, and host specificity of amblyomma aureolatum (acari: ixodidae).in the first part of this study, monthly infestation by ticks was evaluated on dogs from december 2000 to november 2002 in the rural area of taiaçupeba, são paulo. adults of amblyomma aureolatum (pallas) were found on dogs in all months, with a mean prevalence per month of 46.9 +/- 15.7% (range, 25-80%). the mean tick relative abundance per month was 2.4 +/- 2.7 ticks (range, 0.5-14 ticks), and the mean tick mean intensity per month was 4.7 +/- 4.2 ticks (range, 1.5-23.3 ticks). no a. aureolatum ...200415185932
rickettsial infection in animals and brazilian spotted fever endemicity.we compared the rickettsial infection status of amblyomma cajennense ticks, humans, dogs, and horses in both brazilian spotted fever (bsf)-endemic and -nonendemic areas in the state of sao paulo, brazil. most of the horses and few dogs from bsf-endemic areas had serologic titers against rickettsia rickettsii antigens. in contrast, no dogs or horses from bsf-nonendemic areas had serologic titers against r. rickettsii antigens, although they were continually exposed to a. cajennense ticks. all hum ...200515752445
redescription of amblyomma fuscum neumann, 1907 (acari: ixodidae), a rare south america tick confirmed in brazil.the species amblyomma fuscum neumann, 1907 is a rare tick found on the neotropical region, but it has not been recorded as a valid taxon in some lists proposed by current taxonomists. after a comparison between the brazilian material of a. fuscum deposited in the acari collection of the butantan institute, são paulo, brazil, and the male type deposited in leiden museum of natural history, the netherlands, we confirm the taxonomic validity of a. fuscum and redescribe the adult specimens based on ...200515980961
[rickettsiae detection in amblyomma ticks (acari: ixodidae) collected in the urban area of campinas city, sp].the city of campinas is located in an endemic area for brazilian spotted fever in são paulo state, where several cases have recently occurred. capybaras have been associated with the cycle of this disease, for they present positive serology and serve as host for ticks of the genus amblyomma, the main vectors of brazilian spotted fever. ticks were collected both from capybaras and from the vegetation in the city park lago do café, located in the urban area of campinas city, sp, a site associated ...200616501770
a preliminary investigation of ehrlichia species in ticks, humans, dogs, and capybaras from brazil.a molecular epidemiologic investigation in two brazilian states (rondônia and são paulo) was undertaken to determine if ehrlichia species responsible for human and animal ehrlichioses in north america could be found in brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. samples, including 376 ticks comprising 9 amblyomma species, 29 capybara (hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile pati ...200716962245
repellent efficacy of formic acid and the abdominal secretion of carpenter ants (hymenoptera: formicidae) against amblyomma ticks (acari: ixodidae).formic acid is a substance produced by some ants for defense, trail marking, and recruitment. some animals are known to rub ants or other arthropods on parts of their plumage or fur to anoint themselves with released substances. a recent study with a semifree-ranging group of capuchin monkeys, cebus apella l., in the tietê ecological park, sao paulo, brazil, an area of occurrence of the tick species amblyomma cajennense (f.), revealed that "anting" with carpenter ants, camponotus rufipes f. (hym ...200717695031
ticks collected on birds in the state of são paulo, brazil.the present study reports a collection of amblyomma spp. ticks in birds from several areas of the state of são paulo, brazil. a total of 568 tick specimens (404 larvae, 164 nymphs) were collected from 261 bird specimens. from these ticks, 204 (36%) specimens (94 larvae, 110 nymphs) were reared to the adult stage, being identified as amblyomma longirostre (94 larvae, 90 nymphs), amblyomma calcaratum (13 nymphs), amblyomma nodosum (2 nymphs), and amblyomma cajennense (5 nymphs). additionally, 39 l ...200717882514
rickettsia infection in five areas of the state of são paulo, brazil.this study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of são paulo. eight flea species (adoratopsylla antiquorum antiquorum, ctenocephalides felis felis, polygenis atopus, polygenis rimatus, polygenis roberti roberti, polygenis tripus, rhopalopsyllus lugubris, and rhopalopsyllus lutzi lutzi), and five tick species (amblyomma aureolatum, amblyomma cajennense, amblyomma dubitatum, ixodes loricatus, and rhipicephalus sanguineus) were ...200718094887
molecular and parasitological survey of hepatozoon canis (apicomplexa: hepatozoidae) in dogs from rural area of sao paulo state, brazil.hepatozoon canis is a protozoan that infects dogs and is transmitted by the ingestion of the brown dog tick, rhipicephalus sanguineus. two distinct species of hepatozoon genus can infect dogs, h. canis and h. americanum. routine tests to detect the disease are based on direct examination of gametocytes on giemsa-stained blood smears. the objectives of this study were the investigation of infection prevalence in rural area dogs, the comparison of diagnostics by blood smear examination and polymer ...200818188597
serosurvey of rickettsia spp. in dogs and humans from an endemic area for brazilian spotted fever in the state of são paulo, brazil.the present study provides a rickettsial serosurvey in 25 dogs and 35 humans in an endemic area for brazilian spotted fever in the state of são paulo, where the tick amblyomma aureolatum is the main vector. testing canine and human sera by indirect immunofluorescence against four rickettsia antigens (r. rickettsii, r. parkeri, r. felis and r. bellii) showed that 16 (64%) of canine sera and 1 (2.8%) of human sera reacted to at least one of these rickettsial antigens with titers >0r= 64. seven can ...200818278271
rickettsial infection in capybaras (hydrochoerus hydrochaeris) from são paulo, brazil: serological evidence for infection by rickettsia bellii and rickettsia parkeri.in brazil, capybaras (hydrochoerus hydrochaeris) are important hosts for amblyomma ticks, which in turn can transmit rickettsiae to humans and animals. therefore, capybaras are potential sentinels for rickettsial infection.200718320102
[the first report of amblyomma fuscum neumann, 1907 (acari ixodidae) on the lizard tupinambis teguixin (l.) at the municipality of glorinha, state of rio grande do sul, brazil].amblyomma fuscum known only from brazil has been described as a rare tick species with few reports of its occurrence in south and southeast region. this is a new records this tick species (9 females) parasitizing lizard (tupinambis teguixin) at the municipality of glorinha, state of rio grande do sul. the females were deposited in the tick collection of veterinary research institute desiderio finamor (7 specimens), eldorado do sul, rs and in the acari collection from instituto butantan, são paul ...200718373904
ticks (acari: ixodidae) infesting wild birds in an atlantic forest area in the state of são paulo, brazil, with isolation of rickettsia from the tick amblyomma longirostre.during field work in nazaré paulista, state of são paulo, brazil, we found 13 (56.5%) of 23 birds (mostly passeriformes) to be infested by 28 larvae and 1 nymph of amblyomma spp. two larvae were reared to the adult stage, being taxonomically identified as amblyomma parkeri fonseca and aragio, whereas five larvae and one nymph were identified as amblyomma longirostre koch. all six a. longirostre specimens were shown to be infected by rickettsia, as demonstrated by polymerase chain reaction (pcr) ...200818714882
[ticks of genus amblyomma (acari: ixodidae) and their relationship with hosts in endemic area for spotted fever in the state of são paulo].seven species of mammals and 36 of birds were investigated to determine the tick prevalence and intensity of infestation. the study was conducted at the esalq/usp in piracicaba municipality, state of são paulo. it was collected 52 mammals and 158 birds parasitized by 12,418 ticks. adult ticks (n= 7,343) were found on capybaras, while the immature were mainly collected on small mammals and birds. the main hosts for immatures in descending order were opossums (69.1%), capybara (24.4%) and black vu ...200819265580
babesia spp. infection in dogs from rural areas of são paulo state, brazil.the status of babesia spp. infection in dogs from rural areas of são paulo state, brazil was studied. for this, 150 animals were examined by blood smears and by pcr; the presence of tick infestation was also investigated. by the blood smear examination, 3 animals (2%) were detected positive and by pcr for babesia spp. 12 (8%) were positive, with bands visualized in 450 bp. rhipicephalus sanguineus or amblyomma spp. were found on 36 (24%) of the 150 dogs. amblyomma species found were a. cajennens ...200919602312
[survey of rickettsia spp in the ticks amblyomma cajennense and amblyomma dubitatum in the state of são paulo].the presence of rickettsial infection was surveyed in 3,545 amblyomma cajennense ticks and 2,666 amblyomma dubitatum ticks. using the hemolymph test, polymerase chain reaction and isolation of rickettsia in cell cultures, all of the amblyomma cajennense were negative, whereas 634 (23.8%) of the amblyomma dubitatum ticks were shown to be infected with rickettsia bellii.200919684990
prevalence of equine piroplasmosis and its association with tick infestation in the state of são paulo, brazil.serum samples were collected from 582 horses from 40 stud farms in the state of são paulo and tick (acari: ixodidae) infestations were evaluated on them. serum samples were subjected to the complement fixation test (cft) and a competitive inhibition elisa (celisa) for babesia caballi and theileria equi. logistic regression analyses were performed to construct multivariate models that could explain the dependent variable (horses positive for b. caballi or t. equi) as a function of the independent ...200920040201
survey of ticks (acari: ixodidae) and their rickettsia in an atlantic rain forest reserve in the state of são paulo, brazil.the current study investigated the occurrence of ticks and their rickettsiae in the serra do mar state park, which encompasses one of the largest atlantic rain forest reserves of brazil. from july 2008 to june 2009, a total of 2439 ticks (2,196 free living and 243 collected on hosts) was collected, encompassing the following 13 species: amblyomma aureolatum (pallas), amblyomma brasiliense aragao, amblyomma dubitatum neumann, amblyomma fuscum neumann, amblyomma incisum neumann, amblyomma longiros ...201020939390
cross-mating experiments with geographically different populations of amblyomma cajennense (acari: ixodidae).the present study evaluated the reproductive compatibility of the crosses between adult ticks of the following three geographically different populations of amblyomma cajennense: state of são paulo (sp), southeastern brazil; state of rondônia (ro), northern brazil; and colombia (co). in addition, crosses between a. cajennense ticks from argentina (ar) and sp ticks were also performed. the argentinean population (ar) was compatible with sp because their crosses resulted in high % egg hatching (me ...201121286933
rickettsia monteiroi sp. nov., infecting the tick amblyomma incisum in brazil.free-living adult amblyomma incisum ticks were collected in an atlantic rainforest area at intervales state park, state of s+úo paulo, brazil. from an a. incisum specimen, rickettsiae were successfully isolated in vero cell culture by the shell vial technique. rickettsial isolation was confirmed by optical microscopy, transmission electron microscopy, and pcrs targeting portions of the rickettsial genes glta, htra, rrs, and sca1 on infected cells. fragments of 1,089, 457, 1,362, and 443 nucleoti ...201121685169
isolation of rickettsia rickettsii and rickettsia bellii in cell culture from the tick amblyomma aureolatum in brazil.brazilian spotted fever (bsf) is a highly lethal disease caused by rickettsia rickettsii. in the present study, rickettsial infection was evaluated in 669 amblyomma aureolatum adult ticks collected from naturally infested dogs in taiaçupeba, a bsf-endemic area in the state of são paulo. ten (1.49%) ticks were infected with rickettsia bellii, and 6 (0.89%) ticks were infected with r. rickettsii. both rickettsia species were isolated and established in vero cell cultures. the rickettsia isolates w ...200617114770
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