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trouble in the potting shed. 19901973478
problems associated with identification of legionella species from the environment and isolation of six possible new species.following investigation of an outbreak of legionellosis in south australia, numerous legionella-like organisms were isolated from water samples. because of the limited number of commercially available direct fluorescent-antibody reagents and the cross-reactions found with some reagents, non-pneumophila legionellae proved to be difficult to identify and these isolates were stored at -70 degrees c for later study. latex agglutination reagents for legionella pneumophila and legionella anisa develop ...19902317047
legionnaires' disease in south australia, 1979-1988.laboratory investigations supported the diagnosis of legionella pneumonia in 108 patients in south australia over the past 10 years. legionella pneumophila was responsible for 91 infections: the serogroup-1 strain caused 81 of these. l. pneumophila serogroup 2 was the only other strain of l. pneumophila that was isolated from patients; it caused infection in eight patients. in two patients, the serological results did not distinguish between infection with l. pneumophila serogroup 1 and serogrou ...19892687665
legionnaires' disease in south australia. prevalence and diagnosis.legionella pneumophila was successfully isolated from sputum, and from respiratory secretions obtained by tracheal aspiration, of two patients with legionnaires' disease by means of guinea pigs and charcoal yeast extract agar. direct culture of lung tissue from one of these patients gave a pure growth of l. pneumophila. in both cases, legionellas were isolated from specimens which were collected several days after treatment with erythromycin began. direct fluorescent antibody tests on these spec ...19806993890
Legionella steelei sp. nov. isolated from human respiratory specimens in California and South Australia.Legionella-like bacteria were isolated from the respiratory tract of two humans in California and South Australia, but not thought to cause disease. These bacteria, strains F2632 and IMVS-3376, were found to have identical Legionella macrophage infectivity potentiator (mip) sequences, and were therefore further characterized to determine their genetic and phenotypic relatedness and properties. Both of these Gram-negative bacterial strains grew on buffered charcoal yeast extract medium, were cyst ...201121948093
a prospective hospital study of the aetiology of community-acquired pneumonia.in a one-year prospective study of 106 adults (mean age, 60 years) who were admitted to hospital with community-acquired pneumonia, an aetiological diagnosis was made in 82 (77%) patients. streptococcus pneumoniae was considered to be responsible for 44 (42%) and respiratory viruses for 19 (18%) infections. other aetiological agents that were found in a smaller number of patients included haemophilus influenzae (9% of patients), enteric gram-negative bacilli (8% of patients), staphylococcus aure ...19892739613
legionella adelaidensis, a new species isolated from cooling tower water.a legionella-like organism (strain 1762-aus-e) was isolated from a cooling tower of an air-conditioning system in adelaide, south australia, australia. the isolate was presumptively identified as a legionella strain by its growth requirement for l-cysteine and its cellular branched-chain fatty acids. strain 1762-aus-e was serologically distinct in the slide agglutination test with absorbed antisera. dna hybridization confirmed that it is a new legionella species for which the name legionella ade ...19912056032
epidemiological characteristics of legionella infection in south australia: implications for disease control.about one third of adults surveyed in south australia have shown evidence of past silent infection with legionella pneumophila serogroup 1. however, the annual notification rate for symptomatic disease is only about 0.5 per 100,000 residents in non-epidemic years. the male to female ratio is 2.5 to one and approximately 50% of the cases are at least 60 years of age. cases have presented more in summer and in the metropolitan areas. twenty cases of legionnaires' disease occurred during the summer ...19912036080
isolation of legionella longbeachae serogroup 1 from potting mixes.following a statewide outbreak of legionellosis due to legionella longbeachae serogroup 1 in south australia in 1988 and 1989, studies were performed to find a source of the organism. a number of water and soil samples with and without acid decontamination were examined for l. longbeachae by using a selective medium containing vancomycin, aztreonam, and pimafucin. there were no isolations of l. longbeachae from water samples. organisms resembling l. longbeachae were isolated from a number of sam ...19901968736
legionella nagasakiensis sp. nov., isolated from water samples in japan and australia and from a patient with pneumonia in the united states.a novel legionella species was identified based on 16s rrna and mip (macrophage infectivity potentiator) gene sequencing analysis, cellular fatty acids, isoprenoid quinones, biochemical reactions, antigens, and quantitative dna-dna hybridization. the strain cdc-1796-jap-e(t) was isolated from well water at the nagassaki municipal medical center, japan. two strains, cdc-3041-aus-e and cdc-3558-aus-e, were isolated from water samples during an outbreak of legionellosis in south australia. the stra ...201121398499
does using potting mix make you sick? results from a legionella longbeachae case-control study in south australia.a case-control study was performed in south australia to determine if l. longbeachae infection was associated with recent handling of commercial potting mix and to examine possible modes of transmission. twenty-five laboratory-confirmed cases and 75 matched controls were enrolled between april 1997 and march 1999. information on underlying illness, smoking, gardening exposures and behaviours was obtained by telephone interviews. recent use of potting mix was associated with illness (or 4.74, 95% ...200716780608
an outbreak of legionella longbeachae infection in an intensive care unit?during a nine-day period, five patients in a 14-bed intensive care unit (icu) were shown to have seroconverted with a four-fold or greater rise in serum antibody titre to legionella longbeachae serogroup 1. a further two patients were observed to have high titres consistent with previous exposure but earlier serum samples were not available for comparison. no patients had antibody responses to legionella pneumophila serogroups 1 and 2. l. longbeachae was not cultured from respiratory secretions ...200212473468
occurrence and distribution of legionella species in composted plant materials.legionellae were found in many samples of composted plant matter obtained from home gardeners and from facilities which undertook bulk composting. the predominant species isolated from these composts was legionella pneumophila, the strains of which belonged to serogroups other than serogroup 1. other legionella species were present in many samples. legionella longbeachae serogroup 1, which is implicated in human infections in south australia, was present in samples obtained from two of six facil ...199411001749
recurrent legionella longbeachae pneumonia associated with re-exposure to potting soil? 19969028527
legionella waltersii sp. nov. and an unnamed legionella genomospecies isolated from water in australia.two legionella-like organisms were isolated from water samples obtained in adelaide, australia. one organisms was isolated from a drinking water distribution system, and the other was isolated from a cooling tower at a sewage treatment plant. both strains required l-cysteine for growth and contained cellular branched-chain fatty acids and ubiquinones typical of the genus legionella. these strains were serologically distinct from each other as determined by a slide agglutination test. strain 2074 ...19968782669
a model for autumn outbreaks of legionnaires' disease associated with cooling towers, linked to system operation and size.cooling towers have been demonstrated to be amplifiers and disseminators of legionella, the causative organism of legionnaires' disease. community outbreaks associated with cooling towers have been reported with several common factors. small towers (< 300 kw) have predominantly been implicated in outbreaks. cooling tower-associated outbreaks are most frequent in autumn, and frequently implicated systems have been operated after a period of shutdown. this paper reports field study data relating s ...19938405155
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