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[isolation of legionella and free-living amoebae at hot spring spas in kanagawa, japan].microbiological contamination of hot spring bath water is a public health concern. a province-wide survey was carried out to determine the extent and distribution of both legionella and free-living amoebae contamination. among 30 samples of hot spring bath from 12 sites in kanagawa, japan, l. pneumophila was detected in 21 water samples from 11 sites, ranging from 10(1)-10(3) cfu/100 ml. serogroups 3, 5 and 6 of l. pneumophila were predominantly isolated from the samples. naegleria (46.7%), plat ...19989847523
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