| prevalence and risk factor investigation of campylobacter species in beef cattle feces from seven large commercial feedlots in alberta, canada. | this fecal prevalence study targeted cattle from 7 large (10,000 to > 40,000 head) commercial feedlots in alberta as a means of establishing campylobacter levels in cattle just prior to animals entering the food chain. overall, 87% [95% confidence interval (ci) = 86-88] of 2776 fresh pen-floor fecal samples were culture positive for campylobacter species, with prevalences ranging from 76% to 95% among the 7 feedlots. campylobacter spp. prevalence was 88% (95% ci = 86-90) in the summer (n = 1376) ... | 2009 | 20046629 |
| genomics-based molecular epidemiology of campylobacter jejuni isolates from feedlot cattle and from people in alberta, canada. | feedlot cattle in alberta, canada, have been identified as reservoirs for campylobacter jejuni, an important human pathogen. oligonucleotide dna microarrays were used as a platform to compare c. jejuni isolates from feedlot cattle and human clinical cases from alberta. comparative genomic hybridization (cgh) analysis was performed on 87 isolates (46 bovine, 41 human) obtained within the same geographical regions and time frame. thirteen cgh clusters were obtained based on overall comparative gen ... | 2009 | 19036937 |
| antimicrobial drug use and antimicrobial resistance in enteric bacteria among cattle from alberta feedlots. | the purpose of this study was to determine whether antimicrobial resistance (amr) in foodborne pathogens (escherichia coli o157, salmonella, and campylobacter) and non-type-specific e. coli obtained from fecal samples of feedlot cattle was associated with antimicrobial drug (amd) use. a secondary objective was to determine if amr in non-type-specific e. coli could be used as a predictor of amr in foodborne pathogens. fecal samples were collected from pen floors in 21 alberta feedlots during marc ... | 2010 | 19958100 |
| occurrence of foodborne bacteria in alberta feedlots. | the occurrence of generic escherichia coli, e. coli o157, salmonella, and campylobacter in cattle manure, beef carcasses, catch basin water, and soils receiving manure application was determined in 21 alberta feedlots. in cattle manure, generic e. coli (98%, 2069/2100) and campylobacter (76%, 1590/2100) were frequently detected; e. coli o157 (7%, 143/2100) and salmonella (1%, 20/2100) were less frequently detected. samples from beef carcasses in the cooler following hazard analysis critical cont ... | 2009 | 19412396 |
| epidemiology of antimicrobial resistant campylobacter spp. isolated from retail meats in canada. | campylobacter is an important zoonotic pathogen found in livestock and can cause illness in humans following consumption of raw and undercooked meat products. the objectives of this study were to determine the prevalence of campylobacter spp. in retail meat (poultry, turkey, pork and beef) purchased in alberta, canada and to assess antimicrobial resistance and genetic relatedness of recovered campylobacter strains with previously isolated strains from clinical and environmental sources. a compar ... | 2017 | 28477522 |
| Genes coding for virulence determinants of Campylobacter jejuni in human clinical and cattle isolates from Alberta, Canada, and their potential role in colonization of poultry. | Forty nine Campylobacter jejuni isolates from cattle feces collected from Alberta feedlots and 50 clinical C. jejuni isolates from people in Alberta were tested for the presence of 14 genes encoding putative virulence factors by PCR. These included genes implicated in adherence and colonization (flaC, cadF, docC, racR, jlpA, peb1, and dnaJ), invasion (virB11, ciaB, pldA, and iamA) and protection against harsh conditions (htrA, cbrA, and sodB). The genes examined were widely distributed in both t ... | 2011 | 22015699 |
| incidence of antibiotic resistance and characterization of plasmids in campylobacter jejuni strains isolated from clinical sources in alberta, canada. | antibiotic susceptibilities of 382 strains of campylobacter jejuni isolated in alberta, canada, in 1980 and 1981 were determined. although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. tetracycline resistance was noted in 6.8% of strains in 1980 and in 8.6% in 1981. moreover, resistance to high-level tetracycline (32-128 micrograms/ml) was always mediated by a plasmid of 45-50 kilobases. three transmissible ... | 1986 | 3008970 |
| temporal prevalence of antimicrobial resistance in campylobacter spp. from beef cattle in alberta feedlots. | antimicrobial resistance (amr) was temporally assessed in campylobacters isolated from beef cattle (7,738 fecal samples from 2,622 animals) in four commercial feedlots in alberta. all calves were administered chlortetracycline and oxytetracycline in feed, and a majority of the animals (93%) were injected with long-acting oxytetracycline upon arrival at the feedlot. fecal samples from individual animals were collected upon arrival (i.e., entry sample), 69 days (standard deviation [sd] = 3 days) a ... | 2006 | 16751519 |
| a national investigation of the prevalence and diversity of thermophilic campylobacter species in agricultural watersheds in canada. | the occurrence and diversity of thermophilic campylobacter species (c. jejuni, coli, and lari) were studied in water samples from four river basins located across canada. these basins located in quebec (bras d'henri), alberta (oldman), ontario (south nation), and british columbia (sumas) represented some of the most intensive farming areas in canada for hog, beef cattle, dairy cattle, and poultry, respectively. this study analyzed 769 water samples collected from 23 monitoring sites with agricul ... | 2014 | 24930011 |
| incidence of antibiotic resistance in campylobacter jejuni isolated in alberta, canada, from 1999 to 2002, with special reference to tet(o)-mediated tetracycline resistance. | of 203 human clinical isolates of campylobacter jejuni from alberta, canada (1999 to 2002), 101 isolates (50%) were resistant to at least 64 microg of tetracycline/ml, with four isolates exhibiting higher levels of tetracycline resistance (512 microg/ml). in total, the mics for 37% of tetracycline-resistant isolates (256 to 512 microg/ml) were higher than those previously reported in c. jejuni (64 to 128 microg/ml). in the tetracycline-resistant clinical isolates, 67% contained plasmids and all ... | 2004 | 15328109 |
| isolation and characterization of catalase-negative and catalase-weak strains of campylobacter species, including "campylobacter upsaliensis," from humans with gastroenteritis. | during 1987 and 1988, nine strains of catalase-negative or -weak campylobacter species were isolated in alberta, canada. dna hybridization studies demonstrated that seven strains were "campylobacter upsaliensis," one strain was highly homologous with campylobacter jejuni dna, and one strain was a campylobacter unrelated to the other two species. all "c. upsaliensis" strains were hippurate negative, and six of seven were susceptible to cephalothin. the unusual variant of c. jejuni was hippurate p ... | 1989 | 2778067 |