| expression and characterization of bluetongue virus serotype 21 vp7 antigen: c-terminal truncated protein has significantly reduced antigenicity. | in the present study, group-specific antigen vp7 of bluetongue virus (btv) serotype 21 isolated from cattle in tochigi prefecture in japan in 1994 was characterized by sequencing and expression. gene was amplified from cdna synthesized on viral dsrna using reverse-transcriptase-pcr. nucleotide sequence of this isolate showed high similarity with other published btv vp7 sequences. full-length and c-terminal truncated forms of vp7 were expressed in insect cells by a baculovirus gene expression sys ... | 2010 | 21187684 |