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restriction enzyme analysis of vp7 gene of indian isolates of bluetongue virus.the genome segment 7 of two indian isolates of bluetongue virus (btv) from avikanagar (btv-1-western india) and hyderabad (btv-untyped hyderabad-southern india) was amplified by rt-pcr using two sets of vp7 specific primers. a sequence of 1137 bp comprising the complete coding sequence of the vp7 gene from avikanagar isolate and a 1154 bp full-length sequence from btv-ut hyderabad isolate were amplified. further, restriction enzyme digestion of these full-length amplicons, using ecori, psti and ...200919382719
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