prevalence of antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, bovine herpesvirus-1, and bovine parainfluenza-3 virus in sheep and goats in quebec.serum samples were collected from 1,075 clinically normal sheep and goats from 77 flocks in 7 agricultural regions of quebec from june to august 1982. sheep and goats were tested for antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, and bovine herpes-virus-1 by the indirect fluorescent antibody technique and for parainfluenza-3 virus by the hemagglutination inhibition test. the prevalence of antibodies in animals to respiratory syncytial virus was 31%; to bovine vira ...19846089626
bovine respiratory syncytial virus in quebec: antibody prevalence and disease outbreak.the prevalence of antibody to bovine respiratory syncytial virus in quebec and the role of the virus in a respiratory disease outbreak was investigated. the indirect immunofluorescent, neutralization and haemagglutination inhibition techniques were used to carry out this study. of the 1,444 adult animals examined 519 (35.9%) had antibody to bovine respiratory syncytial virus. these positive reactors were found in each agricultural region of quebec. the highest (53.0%) and the lowest (21.8%) prev ...19807000320
the 5'-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus.the 5' untranslated region (utr) of several bovine viral diarrhea virus (bvdv) isolates from the severe quebec outbreak was amplified by polymerase chain reaction (pcr) and sequenced. sequences revealed the loss, for the bvdv type ii isolates, of an internal psti restriction site, which is present in all known bvdv type 1 5' utr sequences. a single restriction enzyme digestion (psti) of an aliquot of pcr product allowed us to differentiate bvdv type i and bvdv type ii.19957646359
a seroepidemiological study of the importance in cow-calf pairs of respiratory and enteric viruses in beef operations from northwestern quebec.serum antibody analyses for bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), bovine coronavirus (bcv), and bovine rotavirus (brv) were performed on 527 randomly selected cows, before calving, and on 407 three-week-old calves. in cows and calves, bcv and brv were the most seroprevalent viruses (80% to 100% according to virus and vaccination status). bovine respiratory syncytial virus was the least seroprevalent in the cows, independ ...19957704839
production effects of pathogens causing bovine leukosis, bovine viral diarrhea, paratuberculosis, and neosporosis.the primary purpose of this research was to determine associations among seropositivity for bovine leukemia virus (blv), bovine viral diarrhea virus (bvdv), mycobacterium avium ssp. paratuberculosis (map), and neospora caninum (nc) and each of 3 outcome variables (305-d milk, fat, and protein production) in canadian dairy cattle. serum samples from up to 30 randomly selected cows from 342 herds on monthly milk testing were tested for antibodies against blv (idexx elisa; idexx corporation, westbr ...200717235141
comparison of humoral immune responses in dairy heifers vaccinated with 3 different commercial vaccines against bovine viral diarrhea virus and bovine herpesvirus-1.a randomized clinical trial was conducted to compare the humoral immune response to 3 different commercial vaccines in dairy heifers housed in 3 different dairy farms in quebec. all heifers were seronegative to type 1 bovine viral diarrhea virus (bvdv) (singer strain), type 2 bvdv (nvsl 125c strain), and bovine herpesvirus-1 (bhv-1) at the beginning of the trial. in addition, control heifers in group 1 remained seronegative to the 2 viruses till the end of the trial. significant differences in h ...200314601677
antigenic variation among bovine viral diarrhea virus (bvdv) strains and the role of different cell fixation methods in immunoassays.antigenic variation among 13 quebec isolates of bovine viral diarrhea virus (bvdv), 4 reference strains and 2 american isolates were studied by peroxidase-linked antibody assay (pla assay) and neutralization test (nt). the quebec strains consisted of 3 isolates before 1993 and 10 isolates from 1993. in the pla assay, we compared 2 different fixatives, acetone and formalin. acetone-fixation allowed us to identify 6 groups from amongst the viruses tested. all the quebec isolates were different fro ...19979008798
morphologic lesions in type 2 bvdv infections experimentally induced by strain bvdv2-1373 recovered from a field case.widespread outbreaks of severe acute bvdv, some associated with hemorrhagic syndrome (hs), were reported in quebec and ontario in 1993. these outbreaks caused significant economic hardship in infected herds. in the ontario outbreak 150 dairy, 600 beef and 100 milk and grain fed veal herds were affected with losses estimated at $40000-$10000 per herd in lost animals, milk production, abortions and genetics. fever, pneumonia, diarrhea, and sudden death occurred in all age groups of cattle. abortio ...200011042409
presence of antibodies to bovine viral diarrhea-mucosal disease virus (border disease) in sheep and goat flocks in quebec.a seroepidemiological study of border disease was conducted in sheep and goats in various areas of quebec. sera of 10% of animals of selected flocks were collected and specific antibodies against bovine viral diarrhea- mucosal disease were tested by seroneutralization. results show that 10.9% and 16% of sheep and goats respectively gave a positive reaction. the lower serological prevalence was found in sheep flocks of the sherbrooke area (5.4%) while the highest percentage of positive sera was o ...19846326984
a short incubation serum neutralization test for bovine viral diarrhea virus.a three day serum neutralization (sn) test for the detection of antibodies to bovine viral diarrhea virus (bvdv), which is an improvement on the existing five day test, is described. the improved test results in a more rapid viral cytopathic effect and utilizes madin darby kidney (mdbk) cells, and horse serum as a medium supplement. a comparison of tests utilizing the nadl and the singer strains of bvdv and the use of either secondary bovine kidney cells with calf serum (bkcs) or continuous mdbk ...19921317246
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