| in vitro transposon mutagenesis of an equine herpesvirus 1 genome cloned as a bacterial artificial chromosome. | the 150-kbp genome of the alphaherpesvirus equine herpesvirus 1 (ehv-1) strain hvs25a was cloned as a bacterial artificial chromosome (ehv-1 bac), with mini f plasmid sequences inserted between genes 62 and 63. transfection of ehv-1 bac dna purified from e. coli gave rise to progeny virus that had a similar growth rate and yield in mammalian cell culture to those of parental wild-type ehv-1. using in vitro mutagenesis with a mu transposon, a large library of ehv-1 bac mutants was generated, and ... | 2006 | 16855811 |