Publications

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the prevalence of human isolates of salmonella subspecies ii in southern africa.the salmonella reference centre in johannesburg received 14059 strains of human origin between 1979 and 1984. a significant proportion (6.3%) proved to belong to subspecies ii. the majority were cultured from faecal material, usually associated with symptoms related to the gastrointestinal tract. they comprised 884 isolates, represented by 203 serotypes, of which 45 were new serotypes. the poor hygienic conditions found in many rural areas, together with possible contamination of food and water ...19873549338
incidence of infections with salmonella enteritidis serotypes in black and indian children. a 16-year survey.a 16-year survey (1960--1975) of salmonella infections other than typhoid fever in black and indian children admitted to king edward viii hospital, durban, is reported. the 3,393 patients yielded 3,688 salmonella enteritidis serotypes, mainly typhimurium, johannesburg/duval, manhattan, enteritidis, and senftenberg. organisms were isolated from one or more of the following: stool, urine, blood, cerebrospinal fluid, liver, sputum, subcutaneous tissue, ear and bone. patients' ages varied from 1 day ...1978366775
microbiological investigation of meat wholesale premises and beef carcases in johannesburg.microbiological surveillance by swabbing meat wholesaler premises revealed ineffective cleaning and build-up of bacteria. proper cleaning, sanitation and handling resulted in a vast improvement during 1975-77. beef samples from the neck of carcases in the wholesale trade were investigated by microbiological methods. excessive total bacterial counts were obtained from numerous carcases. most carcases carried coliform organisms. roughly 90% of carcases were contaminated with e. coli i; counts exce ...1977202708
campylobacter enteritis in johannesburg.in order to establish a reasonable protocol for a diagnostic laboratory we conducted a survey during which we confined the routine culture of stool samples for campylobacter fetus to two groups--all infants under 2 years of age, and older children and adults with obviously diarrhoeic stools. camp. fetus was isolated from 100 of 2323 stool specimens (4,3%). this is within the 3 - 8% isolation rates previously reported from surveys in which all specimens were cultured. camp. fetus isolates represe ...19817455853
the prevalence of intestinal salmonella infection in horses submitted for necropsy.specimens from the ileum, colon and rectum were aseptically collected from 50 consecutive horse carcases submitted for necropsy to the department of pathology, faculty of veterinary science, university of pretoria. these were bacteriologically examined for the presence of salmonella. seventeen of these were positive for salmonella at one or more sites. serotyping of the isolates revealed a dominance of salmonella hayindogo in these horses.19958539039
bacterial populations associated with a sorghum-based fermented weaning cereal.microbiological surveys, to determine the quality and safety, were conducted on 45 sorghum samples comprising dry powders (n = 15) and corresponding fermented (n = 15) and cooked fermented porridge (n = 15) samples collected from households in an informal settlement of the gauteng province of south africa. mean aerobic plate counts, gram-negative counts and bacterial spore counts of sorghum powder samples decreased in fermented and cooked fermented porridge samples. however, mean lactic acid bac ...199910477073
phage types of salmonella enterica serovar enteritidis isolated in south africa from 1991-1995.a total of 615 strains of salmonella enterica serovar enteritidis (se), received from 1991-1995 at the onderstepoort veterinary institute (ovi), were phage typed. most se isolates (54,7%) originated from poultry followed by humans (28,5 %) and poultry eggs (9,6 %). phage type 34 was the most prevalent (40,5 %) of all isolates, followed by phage type 4 (33,8 %). other phage types identified were 1, lb, 4a, 7, 7a, 9a, 14, 24, 24var and 35 (in total 2,4% of isolates). most isolates of se were recei ...200011028749
microbiological quality and safety of ready-to-eat street-vended foods in johannesburg, south africa.fifty-one ready-to-eat street foods, 18 dishwater, and 18 surface swab samples were collected from six vendors in johannesburg, south africa. food temperatures were recorded at the time of sampling. standard methods were used to determine aerobic plate counts (apcs), spore counts (scs), and enterobacteriaceae counts (ecs) for food samples as well as coliform counts (ccs) for water and swab samples. in addition, petrifilm escherichia coli count (pc) plates were used for the enumeration of colifor ...199910571317
the role of enteropathogenic bacteria and viruses in acute diarrhoeal disorders of infancy and childhood in johannesburg. i. summer diarrhoea. 196314130266
the role of enteropathogenic bacteria and viruses in acute diarrhoeal disorders of infancy and childhood in johannesburg. ii. 'non-specific' gastro-enteritis. 196314130267
a study of summer diarrhoea in white children in johannesburg. 196414208564
genotypic and demographic characterization of invasive isolates of salmonella typhimurium in hiv co-infected patients in south africa.non-typhoidal salmonella are frequently food-borne zoonotic pathogens that may cause invasive disease in hiv-positive individuals.200919801800
cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments.this study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in johannesburg, south africa. samples of each cleaning or handling tool were prepared for aerobic (apc), coliform (cc), escherichia coli (ec), bacillus cereus (bcc), and staphylococcus aureus (sac) counts, as ...200718095448
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