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serological survey of rhodococcus equi infection in horses in hokkaido.serological survey of rhodococcus equi infection in horses in hokkaido was performed using elisa. of 2,879 horse sera, 318 (11.0%) gave antibody-positive (od greater than or equal to 0.3) reactions. the antibody-positive rate of female was significantly higher (p less than 0.01) than that of male, and no statistical difference between anglo-arab and thoroughbred was detected in the antibody-positive rate. the maximum antibody-positive rate (27.1%) was shown at 14 years of age. the antibody-posit ...19921391174
antibody responses of japanese horses to influenza viruses in the past few years.a total of 305 horse sera collected in the hidaka district of hokkaido in the years 1988-90 were tested for the presence of hemagglutination-inhibition (hi) antibodies to a/equine/newmarket/1/77 (h7n7), a/equine/tokyo/2/71 (h3n8) and a/equine/kentucky/1/81 (h3n8, kentucky) strains of equine influenza (ei) virus. antibodies to the 3 strains were detected in hardly of the 45 sera from 2-years-old horses which were collected before vaccination. many of the 51 horses, after vaccination with inactiva ...19938461424
seroepidemiological survey of chlamydial infections in light horses in japan.to investigate the overall prevalence of chlamydial infections in light (i.e. non-draught) horses in japan, 599 sera obtained from 12 localities in 1991 were tested for complement fixation antibodies. the mean antibody positive rates of the all sera were 15.2% (91/599) and the regional positive rates were higher in honshu (19.1%, 48/251) and kyushu (20.0%, 20/100) than in hokkaido (9.3%, 23/248). in honshu, the highest rate (56.0%, 28/50) was observed in utsunomiya. analysis of the positive rate ...19938513020
isolation of streptococcus equi subsp. equi from thoroughbred horses in a racehorse-breeding area of japan.for determination whether strangles has invaded the hidaka district of hokkaido, the main racehorse-breeding area of japan, a epizootiological survey with bacterial isolation was carried out during the breeding season in 1995. streptococcus equi subsp. equi, which is the causative agent of strangles, was isolated from two thoroughbred horses with submandibular lymphadenitis. isolates were identified by serological grouping, biochemical tests and analysis of cell surface proteins by western immun ...19979409520
isolation of tick-borne encephalitis virus from wild rodents and a seroepizootiologic survey in hokkaido, japan.to determine the vertebrate host of tick-borne encephalitis (tbe) virus in the southern part of hokkaido, japan, virus isolation was performed using spleens from small mammals captured in the area. two virus strains were isolated, one strain from apodemus speciosus and another from clethrionomys rufocanus. virus isolates were inoculated onto baby hamster kidney cell monolayers and antigen slides were prepared for an indirect immunofluorescent antibody assay. two isolates were identified as tbe v ...199910072153
isolation of virulent rhodococcus equi from native japanese horses.r. equi was isolated from soil samples obtained from the environment of seven native japanese horse breeds (hokkaido, kiso, noma, misaki, tokara, miyako and yonaguni) and from fecal samples collected from three native horse breeds (hokkaido, kiso and misaki). virulent r. equi at various levels (ranging from 0.5 to 12.9%) was isolated from the feces or soil environment of hokkaido, kiso and misaki horses. isolates were investigated both for the presence of 15- to 17-kda antigens (virulence-associ ...200111247045
[epidemiological study of japanese encephalitis in hokkaido. 1. historical review of the occurrence of japanese encephalitis in man and domestic animals]. 19714335384
equine infectious anemia occurring in hokkaido, japan--its histopathology and a critical view of the occurrence and diagnosis of this disease. 19674294930
contagious equine metritis eradicated from japan.contagious equine metritis (cem), a contagious venereal disease of horses, invaded japan in 1980 and spread in the thoroughbred population of the hidaka-iburi district of hokkaido. to eradicate cem, we ran a program aimed at detecting taylorella equigenitalis, the causal agent, in carrier horses by using the pcr test, followed by culling or treatment. in 2001, the first year of the program, 12,356 thoroughbred racing stallions and mares were tested and 11 carriers were found. four, two, one, and ...201122123306
antimicrobial resistance and genetic characteristics of salmonella typhimurium isolated from horses in hokkaido, japan.in this study, we examined the antimicrobial susceptibility of 16 salmonella typhimurium isolates obtained from horses, and applied several genetic methods, namely polymerase chain reaction (pcr) for detecting class 1 integrons, multiplex pcr for detecting multidrug resistant s. typhimurium definitive phage type 104 (mr-dt104), and fluorescent amplified-fragment length polymorphism (faflp). seven isolates with an ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline (acssut) t ...200919721370
nosological study of borna disease virus infection in race horses.to investigate the prevalence of diseases in the borna disease virus (bdv) antibody positive race horses, we undertook seroepidemiological studies of bdv infection on 125 culled race horses in hokkaido, japan. the serological study disclosed the presence of antibodies only to bdv-p40 or -p24 in 19.2% (24/125) and 3.2% (4/125) of culled horses, respectively. antibodies to both bdv-p40 and -p24 were found in 24.0% (30/125) of these horses. of particular note was the finding that locomotorium disor ...200211750144
antigenic and genetic analysis of h3n8 influenza viruses isolated from horses in japan and mongolia, and imported from canada and belgium during 2007-2010.a/equine/kanazawa/1/2007 (h3n8), a/equine/hokkaido/i828/2008 (h3n8) and a/equine/mongolia/1/2008 (h3n8) were isolated from infected horses. a/equine/yokohama/aq19/2009 (h3n8) and a/equine/yokohama/aq13/2010 (h3n8) were isolated from horses imported from canada and belgium examined at the animal quarantine service in yokohama, japan. in the present study, these five isolates were genetically and antigenically analyzed. phylogenetic analysis of hemagglutinin (ha) and neuraminidase (na) genes showe ...201121505822
[a sero-epizootiological survey on influenza among horses in hokkaido, japan]. 19724499988
first incidence of multilocular echinococcosis in a race horse in japan.nodular hepatic lesions caused by larval echinococcus multilocularis were found in a four-year-old female thoroughbred race horse born and raised in hokkaido. before detection of the infection, the infected animal was transported around honshu for racing. the present disclosure of hydatidosis in a race horse raises concern over the potential danger of spreading the infection throughout japan by horses possibly infected in hokkaido.19938286549
occurrence of equine dermatophytosis in hokkaido. 19817321357
isolation of ortho- and paramyxoviruses from migrating feral ducks in hokkaido. brief report.a total of 18 hemagglutinating agents were isolated from 14 of 278 migrating feral ducks in hokkaido during the surveillance studies conducted from 1978 to 1981. seven of the 18 isolates belonged to paramyxovirus and the rest to influenza a virus. five isolates of paramyxovirus reacted specifically with antiserum to duck/hk/199/77 and 7 isolates of influenza a virus possessed the antigenic configuration of h10n3. three of the isolates possessed an hemagglutinin that has no antigenic relation to ...19827165509
genotyping of isolates of taylorella equigenitalis from thoroughbred brood mares in japan.profiles of the genomic dna of 104 strains of t. equigenitalis isolated from brood mares with contagious equine metritis in hokkaido during the breeding seasons from 1980 to 1993, as well as those of five strains (ss28, eq56, eq59, eq70 and hh139) previously isolated in japan were examined after restriction digestion and crossed-field gel electrophoresis. these profiles were essentially identical to each other and the various isolates and strains appeared to have a common genotype, designated 'g ...19958540238
prevalence of antibodies against getah virus in horses raised in hokkaido. 19816276610
efficacy of japanese encephalitis vaccine in horses.the efficacy of japanese encephalitis vaccine in horses has been described from the effect of mass vaccination on the local prevalence of the disease in horses in each district of hokkaido, japan.1976182487
prevalence and distribution of antibodies to getah and japanese encephalitis viruses in horses raised in hokkaido. 19826304391
has echinococcus granulosus settled in hokkaido? 200011056566
an outbreak of contagious equine metritis in japan: isolation of haemophilus equigenitalis from thoroughbred mares with genital infection in hokkaido. 19827098231
evaluating river water quality through land use analysis and n budget approaches in livestock farming areas.this study was carried out to evaluate the quality of river water by analysis of land use in drainage basins and by estimating the n budgets. the drainage basins of shibetsu river (shibetsu area) and bekkanbeushi river (akkeshi area) in eastern hokkaido, japan were selected for a case study, and the evaluation of water quality was up-scaled to the regional level in hokkaido by using the arcview/gis and statistical information. water sampling was carried out in august 2001 and may 2002 in the shi ...200415262158
survey on antibody to getah virus in horses in japan.a seroepidemiological survey was performed on antibody against getah virus in horses in japan by the complement fixation test. the positive rate was 35 and 53% in two areas where an outbreak of the infectious disease was reported, whereas it was in a range of 3.3 to 24.2% in other areas, except in certain prefectures of the kyushu district where a high positive rate was observed. in the hokkaido district, the northernmost part of japan, no reactors were found in horses under 6 years old, unlike ...19806255345
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