prevalence of giardia and cryptosporidium in beef cows in southern ontario and in beef calves in southern british 1998 and 1999, fecal samples were collected from 669 beef cows on 39 farms located within 10 counties of ontario. overall prevalences of giardia, cryptosporidium muris, and cryptosporidium parvum in cows were 8.7%, 10.6%, and 18.4%, respectively. of the 39 farms sampled, giardia was detected on 64%, cr. muris on 72%, and cr. parvum on 90%. cryptosporidium parvum was detected in 28% of the cows in 1998 and in 5.2% in 1999. differences between the 2 y were attributed to sampling during calving ...200515759828
multiplex assay detection of immunoglobulin g antibodies that recognize giardia intestinalis and cryptosporidium parvum antigens.giardiasis and cryptosporidiosis are common enteric parasitic diseases that have similar routes of transmission. in this work, we have identified epitopes within the giardia variant-specific surface protein (vsp) sequences that are recognized by igg antibodies from 13 of 14 (93%) sera from patients with stool-confirmed giardiasis. the conserved epitopes are shared among vsps from both of the assemblages that commonly infect humans, and they are likely to be structural, as both sodium dodecyl sul ...201020876825
microbial quality of reclaimed water for urban reuses: probabilistic risk-based investigation and recommendations.although canada has abundant freshwater resources, many cities still experience seasonal water shortage. supply-side and demand-side management is a core strategy to address this water shortage. under this strategy, reclaimed water, which the canadian public is willing to use for non-potable purposes, is an option. however, no universal guidelines exist for reclaimed water use. despite the federal government's long-term goal to develop guidelines for many water reuse applications, guidelines hav ...201727810759
molecular epidemiology of cryptosporidiosis outbreaks and transmission in british columbia, canada.isolates from 25 (13 sporadic and 12 outbreak) cryptosporidiosis cases, 24 of which were from british columbia, canada, were characterized using nested polymerase chain reaction amplification of the polymorphic internal transcribed spacer 1 locus. two predominant cryptosporidium parvum genotypes were found. twelve (8 sporadic and 4 outbreak) isolates amplified with the cry7/cry21 primer pair and 12 (5 sporadic and 7 outbreak) isolates amplified with the cry7/cryits1 primer pair. multi-locus gene ...199910432058
giardia and cryptosporidium in dairy calves in british columbia.a study was undertaken to determine the prevalence of giardia infections in dairy calves and to compare giardia and cryptosporidium infections in calves of different ages. fresh fecal samples were collected from 386 male and female holstein calves (newborn to 24 wk) in 20 dairies located in the lower fraser river valley area of british columbia. giardia intestinalis, cryptosporidium parvum, and cryptosporidium muris were enumerated in each sample after concentration by sucrose gradient centrifug ...19979360789
enzyme immunoassay of cryptosporidium-specific immunoglobulin g antibodies to assess longitudinal infection trends in six communities in british columbia, canada.a newly developed enzyme-linked immunosorbent assay (elisa) that detects immunoglobulin g antibodies to the 27-kda cryptosporidium parvum sporozoite surface antigen was used to test 4,097 sera collected from pregnant women in 6 communities in british columbia, canada, between january 1996, and december 1997. waterborne outbreaks of cryptosporidiosis occurred in two of the study communities during the period of follow-up, and elisa seropositivity was high in all six communities during the study p ...200516103592
epidemic and endemic seroprevalence of antibodies to cryptosporidium and giardia in residents of three communities with different drinking water supplies.this study was carried out to compare cryptosporidiosis and giardiasis seroprevalence rates in residents of three communities. community (com 1) uses drinking water from deep wells, community 2 (com 2) uses surface water from a protected watershed, and community 3 (com 3) uses surface water frequently containing cryptosporidium oocysts and giardia cysts. unfiltered drinking water from each community was collected at the tap and tested for cryptosporidium oocysts and giardia cysts during the 12 m ...199910348231
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