detection of the cryptosporidium parvum "human" genotype in a dugong (dugong dugon).the cryptosporidium "human" genotype was identified in a paraffin-embedded tissue section from a dugong (dugong dugon) by 2 independent laboratories. dna sequencing and polymerase chain reaction/restriction fragment length polymorphism analysis of the 18s ribosomal rna gene and the acetyl coa synthethase gene clearly identified the genotype as that of the cryptosporidium variant that infects humans. this is the first report of the human cryptosporidium genotype in a nonprimate host.200011191916
novel cryptosporidium genotype in wild australian mice (mus domesticus).a total of 250 mouse fecal specimens collected from crop farms in queensland, australia, were screened for the presence of cryptosporidium spp. using pcr. of these, 19 positives were detected and characterized at a number of loci, including the 18s rrna gene, the acetyl coenzyme a gene, and the actin gene. sequence and phylogenetic analyses identified two genotypes: mouse genotype i and a novel genotype (mouse genotype ii), which is likely to be a valid species. cryptosporidium parvum, which is ...200717921267
implications of faecal indicator bacteria for the microbiological assessment of roof-harvested rainwater quality in southeast queensland, australia.the study aimed to evaluate the suitability of escherichia coli, enterococci, and clostridium perfringens for assessing the microbiological quality of roof-harvested rainwater and assessing whether the concentrations of these faecal indicators can be used to predict the presence or absence of specific zoonotic bacterial or protozoan pathogens. from a total of 100 samples tested, 58%, 83%, and 46% of samples were found to be positive for, respectively, e. coli, enterococci, and clostridium perfri ...201020657617
health risk from the use of roof-harvested rainwater in southeast queensland, australia, as potable or nonpotable water, determined using quantitative microbial risk assessment.a total of 214 rainwater samples from 82 tanks were collected in urban southeast queensland (seq) in australia and analyzed for the presence and numbers of zoonotic bacterial and protozoal pathogens using binary pcr and quantitative pcr (qpcr). quantitative microbial risk assessment (qmra) analysis was used to quantify the risk of infection associated with the exposure to potential pathogens from roof-harvested rainwater used as potable or nonpotable water. of the 214 samples tested, 10.7%, 9.8% ...201020851954
fecal indicators and zoonotic pathogens in household drinking water taps fed from rainwater tanks in southeast queensland, this study, the microbiological quality of household tap water samples fed from rainwater tanks was assessed by monitoring the numbers of escherichia coli bacteria and enterococci from 24 households in southeast queensland (seq), australia. quantitative pcr (qpcr) was also used for the quantitative detection of zoonotic pathogens in water samples from rainwater tanks and connected household taps. the numbers of zoonotic pathogens were also estimated in fecal samples from possums and various s ...201222020514
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