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development of one-step real-time rt-pcr assay for detection and quantitation of peste des petits ruminants virus.in this study, a rapid and specific taqman-based, one-step real-time quantitative reverse transcription pcr (qrt-pcr) has been described for the detection of peste des petits ruminants virus (pprv). primers and probe were designed based on the nucleocapsid protein gene sequence. the real-time qrt-pcr assay was able to detect pprv isolates from very distinct geographical areas (africa, middle east and asia). the specificity of the assay was assessed by including rinderpest virus and other morbill ...200818243345
peste des petits ruminants virus in tibet, china.serologic and molecular evidence indicates that peste des petits ruminants virus (pprv) infection has emerged in goats and sheep in the ngari region of southwestern tibet, people's republic of china. phylogenetic analysis confirms that the pprv strain from tibet is classified as lineage 4 and is closely related to viruses currently circulating in neighboring countries of southern asia.200919193278
detection and genetic characterization of peste des petits ruminants virus in free-living bharals (pseudois nayaur) in tibet, china.peste des petits ruminants (ppr) is an important viral disease of sheep and goats. the wildlife hosts of ppr, which may play an important role in the epidemiology of this disease, are not well characterized. the research was undertaken to study the infection of ppr virus (pprv) in free-living bharals (pseudois nayaur) in tibet, china. in 2007, pprv infection was confirmed in two bharals in rutog county of tibet based on clinical signs and detection of pprv rna in tissue samples. in 2008, pprv in ...201120591454
[sequence analysis of the matrix protein and fusion protein genes of a field peste des petits ruminants virus strain from tibet, china].the nucleotide sequences of m and f genes from a field strain of peste des petits ruminants virus (pprv) ("china/tib/gej/07-30") was firstly determined. the m gene was 1 483 nucleotides in length with a single open reading frame (orf), encoding a protein of 335 amino acids. the f gene was 2411 nucleotides in length, encoding a protein of 546 amino acids. the resulting nucleotide sequence and the deduced amino acid sequences were compared with the homologous regions of other pprv isolates. the nu ...201020836385
development of an indirect elisa with artificially synthesized n protein of ppr virus.the full-length gene encoding the nucleocapsid (n) protein of the virus (pprv) responsible for an outbreak of peste des petits ruminants in tibet in 2007 was synthesized in two stages using overlapping pcr without the need for viral genomic cdna as template. the full-length n gene was successfully expressed in escherichia coli, and the purified gene product bound to monoclonal antibody raised against pprv n protein. furthermore, it was able to replace recombinant b-n antigen as the coating antig ...201121242661
major mutation events in structural genes of peste des petits ruminants virus through serial passages in vitro.peste des petits ruminants (ppr) is an highly contagious disease of small ruminants, and caused by peste des petits ruminants virus (pprv), a member of the genus morbillivirus in the family paramyxoviridae. the first outbreak of ppr in china was officially reported in july 2007, when a pprv strain was successfully isolated from a sick goat in tibet, followed by sequencing at a full-genome level (china/tibet/geg/07-30, genbank: fj905304.1). to date, this isolate has been virulently attenuated by ...201626995222
peste des petits ruminants viruses re-emerging in china, 2013-2014.re-emergence of peste des petits ruminants (ppr) was officially reported in xinjiang uygur autonomous region in north-western china in november 2013, and then along with the movements of goats and sheep, this disease rapidly spread to other provinces, autonomous regions and municipalities (p/a/m) of china. a total of 256 ppr-affected counties in 22 p/a/m were identified up to september 2014. phylogenetic analysis revealed that the current circulating strains and tibet strains isolated previously ...201625620455
complete genome sequence of a peste des petits ruminants virus recovered from wild bharal in tibet, china.for the first time, here we announce the complete genome sequence of a field isolate of peste des petits ruminants virus (pprv) derived from macerated rectal tissue of a free living bharal (pseudois nayaur) that displayed clinical disease consistent with severe infection with pprv. further, we compare the full genome of this isolate, termed pprv tibet/bharal/2008, with previously available pprv genomes, including those of virus isolates from domestic small ruminants local to the area where the r ...201222966182
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