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human leptospirosis in a slum area in the city of rio de janeiro, brazil--a serological and epidemiological study.a serologic survey was carried out on slum dwellers in the city of rio de janeiro. a total of 259 serum samples from male and female individuals of different age groups were tested for the presence of antileptospire antibodies by microagglutination. prevalence data were analyzed in relation to the major risk factors present at the site, mainly represented by the presence of carrier animals and the occurrence of frequent floods. of the samples tested, 25% reacted with antigens of different serogr ...19902215233
epidemiological aspects of leptospirosis in a slum area in the city of rio de janeiro, brazil. search for leptospires and specific antibodies in rodents.101 rattus norvegicus and 3 mus musculus were captured in an urban slum of rio de janeiro city and examined for leptospiral infection. direct examination of urine, serology (microagglutination) and culture together gave a positivity rate of 39%. separately direct examination and serology gave positivity rates of 13% and 36% respectively. serogroup icterohaemorrhagiae predominated in the serological tests. leptospires were isolated from 24 of the 104 rodents (23%), but 4 cultures died. the remain ...19883252599
[epidemiology of human leptospirosis, with special reference to greater rio de janeiro, brazil, from 1970 to 1982].from 1970 to 1982, 884 cases of human leptospirosis were serologically diagnosed at the leptospirosis laboratory, fundação oswaldo cruz - fiocruz, by means of the microscopic agglutination test. of these patients, 775 were hospitalized in the city of rio de janeiro, and 109 were reported by other brazilian cities. icterohaemorrhagiae was the presumptive infecting serogroup most commonly encountered in greater rio patients (76.4%) and in the others (41.3%). during this 13-year period, two icteroh ...19873507570
[leptospirosis in animal reproduction: iii. role of the hardjo serovar in bovine leptospirosis in rio de janeiro, brazil].four hundred and five serum samples were drawn from cows with reproductive problems which were not vaccinated against leptospirosis from 21 dairy farms. three distinct geographic regions were determined and the farms were also classified considering the production system, based on technological, zootechnical and sanitary resources. a total of 277 positive reactions were observed, corresponding to 68.39% of the samples. the predominant serovar was hardjo, reactive on 85 samples (20.98%), predomin ...19958552880
leptospirosis antibodies in mammals from rio de janeiro zoo, brazil.serum samples from 77 animals belonging to 38 species and 19 families in rio de janeiro zoo, brazil were tested for antibodies against serovars of leptospira interrogans by microscopic agglutination test. antibodies were detected in serum samples of 37.7% of all animals belonging to 10 families. seropositivity was more common in the carnivora canidae (7/9), procyonidae (5/9) and the edentata myrmecophagidae (5/9). icterohaemorrhagiae was the most common serogroup. seropositivity was less common ...200212443693
factors associated with bovine leptospirosis in rio de janeiro, brazil.serum samples from 379 adult cows with lowered fertility farmed in the rio de janeiro region of brazil. they were examined for leptospira antibodies. a questionnaire was completed for each herd. antibodies were detected in 46.9% of these cows, mainly to serovar hardjo. main risk factor associated with seropositivity was co-grazing with other species, mainly pigs (or = 3.17; p<0.04). absence of or infrequent veterinary assistance (or = 1.74) has also been suggested to be associated to the overall ...200313129675
leptospiral antibodies in wild felines from rio de janeiro zoo, brazil. 200415301770
leptospiral antibodies in captive lion tamarins (leontopithecus sp) in brazil.serum samples from 73 lion tamarins of three species living at the centro de primatologia do rio de janeiro were tested for antibodies against serovars of leptospira interrogans by microscopic agglutination test. antibodies were detected in serum samples of 15% of tested samples. seroreactivity prevalence was lower than that described in other non-human primates and could be explained by the arboreal behaviour of these animals. icterohaemorrhagiae was the most common serogroup.200515848790
a serological study on brucella abortus, caprine arthritis-encephalitis virus and leptospira in dairy goats in rio de janeiro, brazil.in spite of the large number of goats found in several developing tropical countries, milk production remains unsatisfactory. the occurrence of infectious diseases, such as leptospirosis, brucellosis and caprine arthritis-encephalitis (cae) may in part be responsible for sub-optimal production. in this study, 1000 serum samples were tested for leptospirosis, 953 for brucellosis and 562 for cae. all tested flocks presented at least one seroreactive animal for leptospirosis and for cae. reactivity ...200716455276
influence of leptospirosis on reproductive performance of sows in brazil.the purpose of this study was to investigate the association between seropositivity for the most frequent leptospira serovars and reproductive losses in sows in brazil. serum samples from 351 sows from 18 herds (in the state of rio de janeiro, brazil) with low reproductive efficiency were tested (microscopic agglutination) for antibodies against serovars of leptospira. antibodies were detected in serum samples of 66.1% of all sows, most frequently serovar icterohaemorrhagiae (43.1%), followed by ...200616584764
risk factors associated with leptospirosis in dairy goats under tropical conditions in brazil.serum samples from 248 adult dairy goats from 13 flocks with lowered fertility farmed in the rio de janeiro region of brazil were examined for leptospira antibodies by mat with 24 serovars, cut off 100. a questionnaire was completed for each herd. antibodies were detected in 20.8% of these goats, mainly to serovar hardjo. risk factors associated with seroprevalence to leptospirosis were the frequency of professional veterinary supervision (or = 2.35), climate (or = 2.63) and grazing for more tha ...200817543359
identification of leptospira spp. carriers among seroreactive goats and sheep by polymerase chain reaction.few studies were conducted on the diagnosis and control of small ruminants' leptospirosis. thirteen goat herds and seven sheep flocks located in the state of rio de janeiro, brazil, were screened for leptospirosis. from the three herds and three flocks with greatest seroreactivity by mat (microscopic agglutination test), 19 and 40 seropositive goats and sheep, respectively, were selected, and urine samples were collected for bacteriology and pcr. for both species of animals, the most prevalent r ...200919232418
multiplex pcr-based detection of leptospira in environmental water samples obtained from a slum settlement.the aim of this study was to apply a molecular protocol to detect leptospiral dna in environmental water samples. the study was carried out in a peri-urban settlement in petrópolis, state of rio de janeiro. a multiplex pcr method employing the primers lipl32 and 16srrna was used. three out of 100 analysed samples were positive in the multiplex pcr, two were considered to have saprophytic leptospires and one had pathogenic leptospires. the results obtained supported the idea that multiplex pcr ca ...201020512254
[serologic study for detecting anti-leptospira antibodies in rattus norvegicus from duque de caxias, rio de janeiro, brazil].due to the high incidence of leptospirosis human cases in duque de caxias, state of rio de janeiro, the authors captured 47 rats (rattus norvegicus) in order to search agglutinins on them. through serological reactions, 17 positive animals (36.17%) were identified, being 16 to the serovar icterohaemorrhagiae and 1 to the serovar canicola, with titers from 1/100 to 1/400.19938066330
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