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incidence of rotavirus in beef herds in argentina.faecal samples were collected from 177 diarrhoeic and 40 healthy calves from 19 farms in buenos aires state, argentina during 1984 and 1985. samples were examined for rotavirus by enzyme-linked immunosorbent assay (elisa) and polyacrylamide gel electrophoresis (page) of their genomic rna segments. rotavirus was found in 95 samples of diarrhoeic calves (53 per cent) and in three healthy calves (7 per cent). all positive samples were tentatively classified as group a on the basis of electropheroty ...19873035664
[standardization of a microtechnic for passive hemagglutination to detect antibodies against infectious bovine rhinotracheitis].a micromethod for passive hemagglutination is described for the detection of ibr antibodies in serum samples of nine herds. the samples correspond to different zones of the province buenos aires. in animals without clinical signs the proportion of positives was 43.60%; in herds with signs consistent with the disease, this value attained 66.4% positivity (tables 1 and 2). the feasibility of this method is discussed. some variants introduced into the original technique are described.19846101039
tuberculosis in sea lions and fur seals from the south-western atlantic coast.diverse pathological conditions causing the strandings and/or deaths of several species of sea lions and seals on the northern coast of the province of buenos aires are being studied. tuberculosis was diagnosed in six cases of strandings, involving two otariid seal species (one otaria flavescens and five arctocephalus australis), between march 1989 and december 1992. necropsies were performed on all six cases. granulomatous lesions were observed in the prescapular and hepatic lymph nodes. lesion ...19969025145
[seroprevalence of viral infections in llamas (lama glama) in the republic of argentina].this study reports the seroprevalence of bovine viral infections in llamas (lama glama) in argentina. this is the first study made in the country including 390 llamas and testing antibodies against eight viruses. samples were collected from nine farms distributed in three different provinces: buenos aires, córdoba and jujuy. the samples were tested for antibodies against eight viruses known to infect cattle: bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus ...19979229724
[bovine leukemia virus (blv): prevalence in the cuenca lechera mar y sierras from 1994 to 1995].the cuenca lechera mar y sierras (clms) includes about 300 dairy farms located in the counties of tandil, balcarce, juarez, ayacucho, general pueyrredón, gonzalez chavez and necochea, in the province of buenos aires. the purpose of this investigation was to determine the prevalence of infection caused by bovine leukemia virus (blv) in the clms. we investigated the presence of anti-blv antibodies in 4,203 milk samples taken from 73 dairy farms belonging to the clms. an indirect elisa, which is de ...19979411488
serological survey of viral antibodies in llamas (lama glama) in argentina.this study analysed sera from 390 llamas (lama glama) from nine farms located in three different argentine provinces: buenos aires, cordoba and jujuy. the samples were tested for antibodies against 8 virus known to infect cattle: bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus (badv iii), bovine enterovirus (bev), bovine rotavirus (brv), bluetongue virus (btv), bovine leukaemia virus (blv), and foot-and-mouth virus (fmdv) by conventional methods such as s ...199910337237
health evaluation of pampas deer (ozotoceros bezoarticus celer) at campos del tuyú wildlife reserve, argentina.samples from 14 free-ranging pampas deer (ozotoceros bezoarticus celer) were collected in 1995 and 1998, at campos del tuyú wildlife reserve, buenos aires, argentina. hematology, serum chemistries, minerals and metals, and fecal parasites were analyzed. in addition, fecal ova and parasites were evaluated seasonally during 1998-2000. serology for infectious diseases included blue-tongue, brucellosis, bovine respiratory syncytial virus infection, bovine viral diarrhea/mucosal disease, infectious b ...200314733285
[a study of the epidemiological behaviour of bovine paratuberculosis using time series in tandil in the province of buenos aires, argentina].the authors present the results of a study conducted using time series data from the 1993--2001 period in order to characterise the clinical behaviour of bovine paratuberculosis. the case data, confirmed by macroscopic examination, enzyme-linked immunosorbent assay (elisa) and comparative tuberculin test, came from the herd health register, located in tandil in the province of buenos aires, argentina. the upper limit of customary variation was observed in april, with an incidence of 2.5%. in 199 ...200415861874
cryptosporidium infection in calves from a rural area of buenos aires, argentina.dairy calves less than 1 month of age are commonly infected with cryptosporidium spp. the objective of the present study was to evaluate the prevalence of cryptosporidium spp. among dairy calves <or=30 days old, with and without diarrhoea, in a rural area of buenos aires, argentina. a total of 280 calves were studied. for each calf a single faecal sample was obtained and processed by water-ether concentration. the microscopic search was carried out using a modified ziehl-neelsen's acid fast meth ...200818848398
mycobacterium bovis in argentina: isolates from cats typified by spoligotyping.in the present work, 19 mycobacterium bovis isolates from different cats were typified by spoligotyping. we detected nine spoligotypes. there was only one cluster, which grouped 11 of the isolates (57.9%), showing the main spoligotype from cattle from argentina. the rest of the spoligotypes presented only one isolate each. five of them were not found in cattle, and were unique and exclusive of cats. the isolates studied show that tuberculosis of bovine origin in cats constitutes a potential publ ...200920085184
serologic profiles for sarcocystis sp. and neospora caninum and productive performance in naturally infected beef calves.sarcocystis sp. and neospora caninum infections affect cattle worldwide causing important economic losses. the objective of the present study was to trace serologic profiles for sarcocystis sp. and n. caninum in naturally infected beef calves and analyze their relationship with transmission routes and productive performance. samples were collected in two cow-calf operations located in buenos aires province, argentina. in farm 1, 43 calves were bled and weighed three times. in farm 2, 69 calves w ...201020101411
herd demographics correlated with the spatial distribution of a foot-and-mouth disease epidemic in buenos aires province, argentina.during a recent foot-and-mouth disease epidemic in argentina, cattle herds affected in 2001 were located mainly (69%) in buenos aires province. the densities of outbreaks (no. of outbreaks per km2) and cattle-demographic variables in the province were estimated using a geographical information system and kernel function. before the epidemic officially was recognized, the density of outbreaks was correlated (rsp = 0.28-0.47) with the geographic distribution of small (< or =100 cattle), dairy and ...200415488273
the population ecology of muscina stabulans (fallén) (diptera: muscidae), along an urban-rural gradient of buenos aires, argentina.the false stable fly, muscina stabulans (fallén), was surveyed along an urban-rural gradient at almirante brown, buenos aires province, argentina. samples were taken at monthly intervals from may 2005 to april 2007. hourly captures of adult flies (10:00 am-04:00 pm) were taken at each sampling date with a hand net. the baits used were 250 g of rotten cow liver and 250 g of fresh dog faeces exposed in shaded and sunny areas. the entire sample accumulated 358 specimens. the number of specimens cap ...201020676520
[retrospective analysis of cases with a diagnosis of cerebrocortical necrosis and its relation with type 5 bovine herpesvirus].in order to demonstrate the association of bovine herpesvirus type 5 (bhv-5) and cerebrocortical necrosis (ccn), 89 such cases were examined in cattle from three regions of buenos aires province, argentina, registered between 1970-1999. hematoxylin-eosin staining and bhv-5 in situ hybridization were performed on paraffin-embedded neural tissues. the severity of microscopic lesions was scored according to a 0-3 scale. morbidity, mortality and lethality rates between groups depending on age and re ...200312920986
occurrence and characteristics of cs31a antigen-producing escherichia coli in calves with diarrhoea and septicaemia in argentina.cs31a is a k88-related non-fimbrial adhesin first described on escherichia coli strains isolated from diarrhoeic and septicaemic calves. in this report, cs31a antigen was screened by immunological methods and confirmed by pcr among bovine e. coli isolates. in addition, cs31a-producing strains were characterized with respect to different fimbrial antigens, o-serogroup and other properties related to virulence. faecal or tissue specimens of 100 diarrhoeic or septicaemic calves and 27 older cattle ...200312710494
study of the kinetics of antibodies titres against viral pathogens and detection of rotavirus and parainfluenza 3 infections in captive crias of guanacos (lama guanicoe).a longitudinal study was conducted to investigate the presence of antibodies (ab) to rotavirus (rv), parainfluenza-3 virus (pi-3), bovine herpesvirus-1 (bohv-1), bovine viral diarrhoea virus (bvdv-1) and bluetongue virus (btv) in eleven guanaco's crias (chulengos) relocated from rio negro to buenos aires province (argentina) and reared in captivity for a year in an experimental field. serum samples were collected periodically to detect the evidence of viral infections. faecal samples were collec ...201021062425
dna typing in a cattle stealing case.dna profiling was used as probative evidence in a cattle stealing case. the carcasses of the dead animals were found from a report and a farmer recognized the remains as those corresponding to the stolen animals by the farm mark on the coat. those remains were collected as reference samples. meat pieces were sequestered from a butchery and then sent to our laboratory by the justice department of buenos aires (argentine) to perform a dna comparative analysis with the reference. matches were found ...200111714164
factors associated with infection by campylobacter fetus in beef herds in the province of buenos aires, argentina.campylobacter fetus is a major venereal pathogen of cattle that is considered to be widespread among the livestock population of argentina. the disease accounts for a 10% reduction in the weaning rate of argentine infected herds and annual losses of $165 million. a case-control, questionnaire-based study was developed with the objective of quantifying the association between c. fetus infection and demographic, husbandry, and sanitary factors in 196 herds located in the province of buenos aires, ...201121737166
[chemical composition of creole cattle meat].proximal composition was determined in meat's creole cattle (cgbc). for this purpose, four raw cuts of cgbc were analyzed: buttock, flank, loin blade steak, and shoulder blade steak. the mentioned names of the meat cuts come from the national meat bureau from the republic of argentina. these cuts were taken from an experimental lot of creole cattle (n = 11) in jujuy province. comparisons were made with data extracted from argentine table of food composition, edited by national nutrition institut ...200011464673
[isolation of mycoplasma bovis during an outbreak of bovine mastitis at a dairy farm in the province of buenos aires. 1st report in the republic of argentina].several mycoplasma species produce various diseases in different animal species. m. bovis has been described as the cause of mastitis, arthritis, pneumonia and infertility in cattle. furthermore, this species has been the most frequently isolated agent producing bovine mastitis. the objective of this study was to isolate and typify mycoplasma strains from a clinical mastitis outbreak in a dairy farm of buenos aires province. a total of 279 samples were studied (276 from pooled quarter milk of co ...200010948823
distribution of is900 restriction fragment length polymorphism types among animal mycobacterium avium subsp. paratuberculosis isolates from argentina and europe.sixty-one mycobacterium avium subsp. paratuberculosis isolates from cattle and deer from the buenos aires province, an important livestock region in argentina, were typed by restriction fragment length polymorphisms (rflp) analysis based on is900. four different rflp patterns (designated 'a', 'b', 'c' and 'e') were identified in bsteii digests of genomic dna. the most frequently observed type, pattern 'a', was found in 46 isolates (75%). the second, pattern 'e', included 8 isolates (13%), while ...199910596808
pyrrolizidine alkaloidosis in argentinian cattle caused by senecio selloi.a herd of 135 aberdeen angus cows were grazing on pastures containing abundant senecio selloi in the eastern region of buenos aires province, argentina. some undiagnosed deaths were observed during 1989. in 1990 the death count raised to 38 animals, all of them showing the same clinical signs and gross and histopathological lesions. the time from the start of signs to death varied from 2 to 6 d. the most conspicuous signs were distention of the abdominal cavity and moderate icterus of mucous mem ...19948066965
[poisoning in cattle caused by blue-green algae].this paper describes a case of poisoning by blue-green algae growing in a fresh water pond in goyena, provincia de buenos aires. the predominating algae were identified as microcystis aeruginosa. they show an intense growth when certain meteorologic condition prevail, in coincidence with certain chemical characteristics of the water. once this growth has occurred, the direction of the wind has a decisive role, because it determines the accumulation on the shore, increasing their concentration an ...19846443833
prevalence of iron deficiency in children aged 9-24 mo from a large urban area of argentina.argentina has been largely considered as having an adequate diet in terms of iron availability. however, children aged 9-24 mo do not meet requirements from their regular diet. the aim of this study was to assess the incidence of iron deficiency in a representative sample of children living in greater buenos aires. hematologic indicators were measured in venous blood from 384 children. weighted incidence of anemia was 46.7%. mean corpuscular volume was less than 73 fl in 28.2% of subjects, free ...19902393012
[1st isolation of strains of staphylococcus aureus producing toxic shock syndrome toxin 1 in food handlers in argentina].thirty nine milk handlers from a factory of dairy products in the province of buenos aires were examined for their nasal carriage of s. aureus strains capable of producing toxic-shock-syndrome toxin-1 (tsst-1). in addition, chance samples of handled foods, crude milk and milky fermented derivates (mfd) were studied. strain isolation was made on mannitol salt agar and on baird-parker agar. typical colonies were identified by their biochemical properties. cultures that were found to be s. aureus w ...19902102013
microbiology of diarrhoea in young beef and dairy calves in argentina.rotavirus, cryptosporidium sp, and salmonella spp. were investigated in the faeces of 452 diarrhoeic calves from 36 beef and 33 dairy herds. animals surveyed were from a few days of age up to approximately 1 month of life. enterotoxigenic escherichia coli (etec) was studied in 212 calves, aged 15 days or less. the animals were from the provinces of buenos aires (59% of the calves), córdoba (18%), santa fe (16%), entre ríos (5%) and la pampa (2%). a minimum of 4 calves were sampled on each farm. ...19902102011
calcium kinetics in the solanum malacoxylon-treated rat.the chronic ingestion of the leaves of the plant solanum malacoxylon (sm) causes an endemic disease in the cattle of some areas of buenos aires province. the animals affected manifest loss of appetite and weight, hypercalcemia, hyperphosphatemia, and ectopic calcifications. in order to study the mechanism of the hypercalcemia provoked by the administration of sm, a calcium kinetic study was performed in control and treated adult intact rats. the animals receiving sm showed higher levels of serum ...19751195017
occurrence of shiga toxin-producing e. coli (stec) on carcasses and retail beef cuts in the marketing chain of beef in argentina.argentina has the highest incidence of hus in the world. hus is produced by stec o157 and non-o157. cattle's faeces and hides are sources of stec contamination of carcasses during slaughter. we investigated the presence of stec in carcasses and cuts of meat in the marketing chain in an agricultural city located in buenos aires province (argentina). in this study, the detection of the stx gene was used as an indicator of carriage of meat with stec. in carcasses, we detected 12.34% and 18.64% of s ...201020646836
mycobiota and mycotoxins in fermented feed, wheat grains and corn grains in southeastern buenos aires province, argentina.wheat (as bran) and corn (as dry grain or fermented feed) are main ingredients of feedstuffs used in local cattle and pig farms in the south of the buenos aires province (argentina). therefore, determining mycobiota and mycotoxins in wheat and corn is of prime importance for developing feed management techniques to optimise animal production and to minimize toxicity. then, a mycological survey was carried out in the southeastern part of the buenos aires province, in order to identify the mycobio ...200919766518
shiga toxin-producing escherichia coli o157 in beef and chicken burgers, and chicken carcasses in buenos aires, argentina.we describe the isolation and characterization of shiga toxin (stx)-producing escherichia coli (stec) o157:h7 from cooked and uncooked beef and chicken burgers and from chicken carcasses collected during sampling procedures in 2001 and 2002 in buenos aires city, argentina. of the 24 stec o157:h7 strains isolated, 20 were recovered from 19 (6.8%) out of 279 samples of beef and chicken burgers, and 4 strains from 4 (10.3%) out of 39 chicken carcasses. the samples were analyzed following the usda/f ...200919443067
flesh flies species (diptera: sarcophagidae) from a grassland and a woodland in a nature reserve of buenos aires, argentina.temporal changes of flesh flies abundance were simultaneously recorded at monthly intervals during a year in a woodland and in a grassland at the "reserva ecológica costanera sur" (recs), buenos aires city, argentina. samples were taken at monthly intervals (february 2004-january 2005). hourly captures of adult flies (10:00 am - 04:00 pm) were taken each sampling date with a hand net. temperature was recorded at each hourly capture. the baits used were 200 g of rotten cow liver and 200 g of fres ...200819419045
effect of leptin gene polymorphisms on growth, slaughter and meat quality traits of grazing brangus steers.leptin is a hormone that affects the regulation of feed intake, energy balance and body composition in mammals. several polymorphisms in the bovine leptin gene have been associated with phenotypic variance of these traits. we evaluated two known single nucleotide polymorphisms (snps) in the leptin gene of 253 grazing brangus steers. brangus is a 5/8 angus-3/8 brahman composite. data were collected during two consecutive growth/fattening cycles from two farms in southeast buenos aires province, a ...200919283678
risk factors associated with tritrichomonas foetus infection in beef herds in the province of buenos aires, argentina.bovine trichomonosis (bt) is a contagious disease, characterized by reproductive failure, embryonic losses, infertility, and abortions, which directly impacts the reproductive performance of cattle. in this cross-sectional questionnaire-based study, 56 veterinarians provided information on the geographic location and on the demographic, husbandry, and sanitary characteristics of 173 beef herds located in 26 (21%) counties of the province of buenos aires, argentina. herds rearing beef cattle (or= ...200818337015
dynamics of eimeria oocyst excretion in dairy calves in the province of buenos aires (argentina), during their first 2 months of age.clinical coccidiosis is associated with high fecal contamination and stress situations, mainly in animals under 1 year of age. artificially fed dairy calves are one of the categories most prone to suffer from this parasitic disease. the study was carried out in a commercial dairy farm. feces samples of heifer calves between 2 and 8 weeks old were taken monthly for oocyst counts and eimeria spp. identification. of the 862 feces samples analyzed, 48% presented oocysts. when grouping the results of ...200818096320
[molecular study of argentine strains of bacillus anthracis].bacillus anthracis is one of the most monomorphic bacteria known and epidemiological studies of this microorganism have been hampered by the lack of molecular markers. for the genotyping of fourteen argentine field strains and the vaccine strain steme 34f2 the presence or absence of the virulence plasmids as well as vrra locus containing a variable-number tandem repeat (vntr) and presenting a polymorphism involving five variants, were analyzed. strains were isolated from cows, sheep and pigs dur ...200717702250
phylogenetic analysis of porcine rotavirus in argentina: increasing diversity of g4 strains and evidence of interspecies transmission.group a rotaviruses are one of the most frequently detected viral agents associated with neonatal diarrhea in piglets. in order to characterize rotavirus (rv) strains circulating in argentinean swine, four porcine production farms located in buenos aires were studied. rv strains genotyped as p[6]g4, p[6]g8 and p[1]g6 were found in piglets under 30 days of age, without diarrhea. phylogenetic and sequence analysis of the vp7 gene from g4 strains available in databases, reveals five porcine new lin ...200817659850
[the epidemiology of hemolytic uremic syndrome in argentina. diagnosis of the etiologic agent, reservoirs and routes of transmission].shiga toxin-producing escherichia coli (stec) cause sporadic cases and outbreaks of nonbloody and bloody diarrhea, and hemolytic uremic syndrome (hus). e. coil o157:h7 is the most prevalent stec serotype. however, other serotypes (o26:h11; o103:h2; o111:nm; o121:h19; o145:nm, among others) can cause a similar disease spectrum. shiga toxins (stx1, stx2, and their variants), intimin, and enterohemolysin are the main virulence factors. three different diagnostic criteria are used to determine the f ...200617354474
a description of cattle movements in two departments of buenos aires province, argentina.we present a descriptive analysis of cattle movement information retrieved from the argentinean animal movement database for two departments in the province of buenos aires during 2004. for each quarter of the year (january to march, april to june, july to september, and october to december) we report the number of on- and off-farm movement events for the purpose of finishing. our analyses show that the distribution of the number of finishing-related movement events per farm was skewed, with the ...200616777252
endometritis treatment with a pgf2alpha analog does not improve reproductive performance in a large dairy herd in argentina.in argentina, most dairy cows with endometritis are treated with prostaglandin (pgf(2alpha) or its analogs) and insemination is withheld until there are no signs of endometritis. the objective of the present study was to evaluate if this method of managing endometritis enhances reproductive performance. three experiments were conducted over 4 years in a large farm in the west of buenos aires province. in experiment 1, half of the cows diagnosed with endometritis (>1.5-fold difference in diameter ...200515725435
subclinical endometritis and its impact on reproductive performance in grazing dairy cattle in argentina.recently several studies have reported that subclinical endometritis impairs reproductive performance in cattle. most of the studies were conducted in western industrialized countries under intensive housing conditions. the objective of the present study was to determine the prevalence of subclinical endometritis and its impact on reproductive performance outcomes in clinically healthy postpartum dairy cows in a pasture-based extensive dairy farming system in argentina. lactating holstein cows ( ...201020727691
prevalence of clinical endometritis and its impact on reproductive performance in grazing dairy cattle in argentina.the objective of this study was to evaluate the prevalence of clinical endometritis and its impact on reproductive performance in grazing dairy cattle in argentina to compare data with previous reports from herds kept in confinement housing systems. a total of 243 holstein dairy cows from three commercial dairy farms in buenos aires province (argentina) were examined for the signs of clinical endometritis 18-38 days postpartum (dpp) by external inspection and manual vaginal examination. vaginal ...201020880160
[a century of cattle ranches in the countryside of buenos aires, 1751-1853]. 199921162334
how much do soil and water contribute to the composition of meat? a case study: meat from three areas of argentina.the main goal of this study was to propose a reliable method to verify the geographical origin of meat, establishing the influence of soil and water on its isotopic and elemental composition. thus, beef meat, soil, and water samples were collected from three major cattle-producing regions of argentina (buenos aires, córdoba, and entre ríos). multielemental composition was determined on these three matrices by inductively coupled plasma mass spectrometry (icp-ms), δ(13)c and δ(15)n by isotope-rat ...201121932864
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