| seroprevalence of anaplasma marginale in cattle in parana state, brazil, by msp-5 competitive elisa. | | 1998 | 9668503 |
| serological diagnosis of neosporosis in a herd of dairy cattle in southern brazil. | neospora caninum-specific antibodies were detected in 60 of 172 (34.8%) dairy cattle by enzyme-linked immunosorbent assay (elisa) in a herd from parana state, brazil. the seropositive animals included 47 of 126 (37.3%) adult cows, 7 of 29 (24%) heifers (1-2 yr), 4 of 15 (27%) heifers (5 mo-1 yr), and 2 precolostral samples. data collected over a 9-yr follow-up period revealed that the proportion of pregnancies ending in abortion was 20% (31/154) among elisa seropositive cows and 8% (15/193) amon ... | 2001 | 11780849 |
| prevalence of neospora caninum antibodies in dogs from dairy cattle farms in parana, brazil. | serum samples from 134 dogs from 22 cattle dairy farms in the northern region of parana state, brazil, were tested for antibodies to neospora caninum in an indirect fluorescent antibody test. antibodies (> or = 1:50) to n. caninum were found in 29 (21.6%) of the 134 dogs, and seropositive dogs were found on 14 (63.6%) of the 22 dairy cattle farrms. the antibody titers of dogs were 1:50 (3 dogs), 1:100 (7 dogs), 1:200 (7 dogs), 1:400 (6 dogs), and > or = 1:800 (6 dogs). the low prevalence (9%) in ... | 2002 | 12054023 |
| isolation of neospora caninum from a blind calf in paraná, southern brazil. | | 2003 | 14533770 |
| prevalence of neospora caninum antibodies and factors associated with their presence in dairy cattle of the north of paraná state, brazil. | to determine the prevalence of neospora caninum antibodies and associated factors, blood sera from 623 female dairy cattle from 23 farms in the north of the state of paraná, brazil, were analyzed by means of the indirect immunofluorescent-antibody test (ifat > or = 25). serum samples from 134 dogs living on the same farms also were tested for n. caninum antibodies (ifat > or = 50), and the presence of dogs was associated with the prevalence of n. caninum antibodies in cattle. the overall seropre ... | 2004 | 15350656 |
| cloning, sequencing, expression, and antigenic characterization of rmsp4 from anaplasma marginale isolated from paraná state, brazil. | anaplasmosis is a bovine intraerythrocytic disease caused by the bacterium anaplasma marginale; it causes significant economic losses in tropical and subtropical regions, worldwide. the msp4 gene of an a. marginale strain isolated in paran , brazil, was amplified by pcr and sequenced; its cloning into the pet102/d-topo vector produced an msp4-6xhis-v5-hp thioredoxin fusion gene construct. this recombinant clone was over-expressed in escherichia coli bl21(de-3); the expressed fusion protein was f ... | 2007 | 17278086 |
| phenotypic and genotypic traits of shiga toxin-producing escherichia coli strains isolated from beef cattle from paraná state, southern brazil. | to investigate the prevalence and characteristics of shiga toxin-producing escherichia coli (stec) in cattle from paraná state, southern brazil. | 2007 | 17576221 |
| [seroprevalence of anaplasma marginale in cattle from center-south region of paraná state, brazil by a competitive elisa test with recombinant msp5-pr1 protein]. | anaplasma marginale prevalence was determined in 223 sera samples in 2-year old or older cattle, from the center-southern region of the paraná state, including ponta grossa, guarapuava and laranjeiras do sul municipalities. a survey of antibodies igg class against anaplasma marginale was performed through a competitive immune absorbent assay (celisa-pr1). from the 223 sera examined, 130 (58.74%) reacted to celisa-pr1 test, suggesting an region of enzootic instability, with a significant percenta ... | 2009 | 19500456 |
| [determination and correlation of anti-neospora caninum antibodies in cattle and dogs from paraná, brazil]. | the protozoan parasite neospora caninum is a major cause of abortion in cattle worldwide. the aim of the present study was to determine and to compare the presence of anti-neospora caninum antibodies in cattle, as well as in farm dogs, and the relationship among them. the research was conducted in different mesoregions of paraná state, southern brazil. sera from 1.263 cattle, from 77 farms were tested for antibodies to n. caninum by elisa. sera from 129 dogs, from 35 farms were tested for antibo ... | 2008 | 20059847 |
| occurrence and antibiotic resistance of coliform bacteria and antimicrobial residues in pasteurized cow's milk from brazil. | in this study we examined the hygienic and sanitary quality of pasteurized cow's milk in the state of paraná, brazil, by determining the presence of coliforms and occurrence of antimicrobial residues. a total of 260 milk samples were collected from commercial establishments in different regions of the state. coliform populations were estimated by the multiple-tube test, and antimicrobial residues were detected by enzyme-linked immunosorbent assay. overall, 105 samples (40.4%) were unsuitable for ... | 2010 | 20828476 |
| neospora caninum: evaluation of vertical transmission in slaughtered beef cows (bos indicus). | neospora caninum is a protozoan parasite that causes the most important reproductive problems in cattle worldwide. the objective of this study was to evaluate the possibility of vertical transmission of n. caninum in zebus breed beef cows (bos indicus) submitted for slaughter at an abattoir in the northern region of the state of paraná, southern brazil. one hundred and fifty-nine cows were evaluated: 83 pregnant (in different stages of gestation) and 76 non-pregnant. serum determination of n. ca ... | 2010 | 21063729 |
| occurrence of antimicrobial residues in pasteurized milk commercialized in the state of paraná, brazil. | milk containing antimicrobial residues presents a health risk to the human population. the objective of this study was to use an enzyme-immunoassay technique to determine the occurrence of antimicrobial residues in 151 samples of pasteurized milk sold in cities of the state of paraná, brazil, from march 2005 to april 2006. fifty-nine (41.3%) of the 151 samples contained antimicrobial residues. residues of neomycin, streptomycin, and/or dihydrostreptomycin and chloramphenicol were found in three, ... | 2009 | 19435250 |
| virulence properties and antimicrobial susceptibility of shiga toxin-producing escherichia coli strains isolated from healthy cattle from paraná state, brazil. | the presence of shiga toxin-producing escherichia coli (stec) strains in feces samples of cattle was determined using the cytotoxicity assay on vero cells and a screening pcr system to detect stx genes. the stec isolates were serotyped, tested for antimicrobial susceptibility, and analyzed for virulence genes using multiplex pcr. the verocytotoxin-producing e. coli - reverse passive latex agglutination (vtec-rpla) assay was also used to detect shiga toxin production. the frequency of cattle shed ... | 2008 | 18641705 |
| distribution, hosts, 16s rdna sequences and phylogenetic position of the neotropical tick amblyomma parvum (acari: ixodidae). | the hosts, distribution, intraspecific genetic variation and phylogenetic position of amblyomma parvum (acari: ixodidae) have recently been re-assessed. data on this tick's hosts and distribution were obtained not only from existing literature but also from unpublished records. sequences of the ticks' mitochondrial 16s ribosomal dna (rdna) were used to evaluate genetic variation among specimens of a. parvum from different localities in argentina and brazil, and to explore the phylogenetic relati ... | 2008 | 18577332 |
| cloning, sequencing and antigenic characterization of rvirb9 of anaplasma marginale isolated from paraná state, brazil. | anaplasma marginale, a tick-borne bacterium, causes bovine anaplasmosis responsible for significant economic losses in tropical and subtropical regions worldwide. various major outer membranes have been described, and virb9, a type iv secretion system protein, has been recently indicated as a candidate in vaccine development against anaplasmosis. the virb9 gene of an a. marginale strain isolated in paraná, brazil, was cloned by polymerase chain reaction and sequenced; its cloning into the petsum ... | 2008 | 18561379 |
| [anatomic regions of major occurrence of cysticercus bovis in bovines under federal inspection at slaughterhouse in the municipality of são josé dos pinhais, state of paraná from july to december, 2000]. | the purpose of this work was to determine the most common anatomic location of bovine cysticercosis, as well as its number per animal. to accomplish these objectives a number of 26,633 bovines from the slaughterhouse argus ltd., sif 1710, located at são josé dos pinhais in the state of paraná, brazil, were examined from july to december 2000. the results demonstrated that the head's muscles were infected with a rate of 57.77%, and the cardiac muscles 39.65%, totalizing 97.42% of the infected sit ... | 2007 | 17706011 |
| frequency of group a rotavirus in diarrhoeic calves in brazilian cattle herds, 1998-2002. | the frequency of group a bovine rotavirus (gpa brv) in calves from 1998 to 2002 was determined by the polyacrylamide gel electrophoresis technique in 2177 faecal samples, of which 1898 samples were diarrhoeic and 279 were of normal consistency (control group) that were collected from asymptomatic calves for comparative purposes. the animals were from beef and dairy cattle herds (n = 321) from 158 counties in seven states (paraná, são paulo, minas gerais, mato grosso do sul, mato grosso, goiás an ... | 2006 | 17265766 |
| phylogenetic analysis of anaplasma marginale strains from paraná state, brazil, using the msp1alpha and msp4 genes. | anaplasma marginale is an obligate intraerythrocytic rickettsial pathogen (order, rickettsiales: family, anaplasmataceae) that causes bovine anaplasmosis. this disease is widely distributed in tropical and sub-tropical regions of the world and causes important economic losses to cattle production. major surface protein (msp)1a (msp1alpha gene) is one of the six msps identified on a. marginale from cattle, whose sequence and size vary according to the number of tandem 28- to 29-amino acid repeats ... | 2006 | 17062116 |
| epidemiology of eurytrematosis (eurytrema spp. trematoda: dicrocoeliidae) in slaughtered beef cattle from the central-west region of the state of paraná, brazil. | bovine eurytrematosis (be) in brazil is caused by eurytrema coelomaticum; the disease results in the condemnation of the pancreas of infected animals. the objective of this study was to describe the epidemiology of eurytrematosis in cattle submitted for slaughter during june 2003 to may 2004 at the municipal abattoir of campo mourão, paraná, brazil, and compare gross observation of infection with histological findings. during the period studied, 1828 pancreases were collected randomly. the avera ... | 2006 | 16860482 |
| [evaluation of sandfly control in lobato municipality, paraná state, southern brazil]. | sandfly control measures were evaluated at the da barra ranch, lobato municipality, paraná state, brazil. the insects were captured with falcão traps in houses, a cattle corral, and a forest area from july to november 1999 and from february to june 2000. in december 1999 and january 2000 the following measures were taken to decrease the sandfly density in peridomiciliary areas and domiciles: (i) screens were placed on windows of buildings (domiciles, dormitory, cafeteria); (ii) all organic mater ... | 2006 | 16501757 |
| genetic structure of blastocerus dichotomus populations in the parana river basin (brazil) based on protein variability. | the population structure of 147 marsh deer (blastocerus dichotomus) from three areas in the paraná river basin, brazil, was studied by observing protein polymorphism at 17 loci. six loci were polymorphic and 11 monomorphic. the proportion of polymorphic loci (p) was 35.29% and the average heterozygosity (h) was 6.31%. wright's fst indicated that only 4.9% of the total variation in allelic frequencies was due to genetic differences between the three groups. the high value of f(is) (0.246) indicat ... | 2005 | 16144298 |
| wasting and mortality in beef cattle parasitized by eurytrema coelomaticum in the state of paraná, southern brazil. | a sporadic wasting syndrome affecting beef cattle herds parasitized by eurytrema coelomaticum is described in the state of paraná, southern brazil. the disease was characterized by progressive weight loss, poor body condition despite plenty of good quality forage available, and death. annual losses ranged from 1 to 3%. the clinical course of the disease varied from 2 to 10 months after the onset of the first clinical signs. at necropsy, one of the three distinct following patterns of lesions wer ... | 2005 | 16046069 |
| bovine herpesvirus type 1 abortions detected by a semi-nested pcr in brazilian cattle herds. | bovine herpesvirus type 1 (bhv-1) was investigated by a semi-nested polymerase chain reaction (sn-pcr) and by mdbk cell culture virus isolation in organ fragments from 55 aborted fetuses collected from beef and dairy cattle herds with history of reproductive problems in the north of paraná state, brazil. a 425 bp amplicon of the bhv-1 glycoprotein d gene was detected in 14 (25.4%) aborted fetuses. bhv-1 was isolated in mdbk cells from the tissue of 5 (9.1%) fetuses. the specificity of positive r ... | 2004 | 15894030 |
| aflatoxin occurrence in foodstuff supplied to dairy cattle and aflatoxin m1 in raw milk in the north of paraná state. | moulds occur in a great variety of foods, including the concentrated and roughage destined for animal feeding, and can produce mycotoxins under certain conditions. the ingestion of mycotoxin afb(1) by dairy cattle leads to the biotransformation of that substance, which is eliminated via milk as afm(1), and also causes damage to human health. the present study aimed to analyze the presence of aflatoxins in foodstuff destined for dairy cattle and in the milk produced by these animals. the contamin ... | 2005 | 15811578 |
| g and p genotypes of group a rotavirus strains circulating in calves in brazil, 1996-1999. | fifty fecal samples from calves with diarrhea, positive for group a rotavirus by enzyme immunoassay (eia) and polyacrylamide gel electrophoresis (page) were analyzed by a nested multiplex reverse transcription/polymerase chain reaction (nested multiplex/rt-pcr) for identification of p and g genotypes. samples were collected between 1996 and 1999 from eight dairy and/or beef cattle herds located in the mato grosso do sul, são paulo, goiás and paraná states, brazil. complete genotyping was possibl ... | 2004 | 15066719 |
| levels of lead in mineral salt commercial mixtures for beef cattle. | lead concentrations was measured in samples of mineral mixtures used in beef cattle feed in the states of sao paulo, paraná, mato grosso and mato grosso do sul, brazil. lead content was determined by inductively coupled plasma atomic emission spectrometry. of the 60 analyzed samples, 21 had values greater than 30 ppm (range less 1.6 to 460 ppm), which is the maximum concentration recommended. these findings show the necessity for careful industrial monitoring because some mineral mixtures contai ... | 2003 | 14685028 |
| detection of cadmium in mineral salt commercial mixtures for beef cattle. | the cadmium concentrations in mineral mixtures used in beef cattle feed in the states of são paulo and paraná, brazil was measured. the cadmium concentration was determined by inductively coupled plasma atomic emission spectrometry. of the 36 analyzed samples, 35 had values > 0.5 ppm (range 0.5 to 11.2 ppm), which is the maximum concentration recommended [4, 18]. these findings show the necessity for careful industrial monitoring, as some mineral mixtures contain sufficient cadmium to cause toxi ... | 2002 | 12441684 |
| forests or fields? a response to the theory that tropical forest conservation poses a threat to the poor. | a rebuttal is provided of david wood's proposal to open up tropical forests to agricultural uses. concern is raised that his proposal exaggerates the ability of forests to regenerate after agricultural uses, sacrifices tropical forests in the name of alleviating poverty and in support of large land owners, and prevents valuable potential resource use by local populations. there is misunderstanding by wood of conservationists' postures and the cause of current deforestation. in brazil, defores ... | 1993 | 12345003 |
| [the brazilguayans, brazilian migrants in paraguay]. | "since the 1950s, the population of eastern regions of paraguay has grown with the steady influx of brazilian immigrants. planters from parana and sao paulo, mechanized wheat and soy farmers from the southern campos and cattle farmers from the matto grosso do sul have joined mixed farmers, descendants of european settlers from the south of brazil. despite some local conflicts, the brazilguayans...[in] oriente [province] seem to be on the road to integration in paraguay." (summary in eng and sp ... | 1994 | 12288169 |
| concentration of lead in mineral salt mixtures used as supplements in cattle food. | we measured lead concentrations in samples of mineral salt mixtures used in cattle feed in the states of paraná and são paulo, brazil. lead content was determined spectrometrically by the atomic plasma induction method. of the 30 samples analyzed, 9 had lead concentrations above 30 ppm (range 35.7 to 284.5 ppm) which is the maximum concentration recommended by the national research council (nrc 1980). these findings show the necessity for careful industrial monitoring because some mineral mixtur ... | 2001 | 11370736 |
| use of repetitive dna elements to define genetic relationships among anaplasma marginale isolates. | anaplasma marginale genomic dna was tested for the presence of repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (eric)-like sequences in order to evaluate the genetic diversity of multiple a. marginale isolates. a. marginale isolates were obtained from cattle of six different states of brazil, from the us and an anaplasma centrale strain was obtained from uruguay. patterns obtained from a. marginale isolates varied from 14 to 17 fragments by rep-polymer ... | 2001 | 11313126 |
| [cholesterol in beef, pork, chicken and their products commercialized in maringá, paraná, brazil]. | a rapid method developed by al-hasani et al. with modifications for cholesterol determination was applied in samples of beef, pork, chicken and meat products. analyses of cholesterol were performed by capillary gas chromatography. the obtained results showed that chicken presented higher concentration (126.96 to 188.29 mg/100 g) of cholesterol in comparison to beef and pork and similar to meat products, except bovine liver that presented 265.03 mg/100 g. cholesterol values of beef and pork sampl ... | 1997 | 9673687 |
| [additional information on fascioliasis hepatica in curitiba (state of paraná, brazil)]. | | 1982 | 7134738 |
| molecular detection of bovine coronavirus in a diarrhea outbreak in pasture-feeding nellore steers in southern brazil. | worldwide diarrhea outbreaks in cattle herds are more frequently detected in calves being that diarrhea outbreaks in adult cattle are not common. winter dysentery (wd) is a bovine coronavirus (bcov) enteric infection that is more reported in northern hemisphere. seasonal outbreaks of wd in adult cattle occur mainly in dairy cows. wd has not been described in beef cattle herds of tropical countries. this study describes the molecular detection of bcov in a diarrhea outbreak in beef cattle steers ... | 2016 | 26712361 |
| mapping risk of bovine fasciolosis in the south of brazil using geographic information systems. | fasciolosis, caused by fasciola hepatica, is an endemic disease of ruminants that occurs in several countries of south america where it can lead to decreased production and fertility and, in severe cases, animal death. although very prevalent, information on the epidemiology of the disease is incomplete in brazil. the objective of the present study was to define the prevalence of f. hepatica in the livers of cattle from slaughterhouses and correlate the data with the animal's origin (climate and ... | 2010 | 20071083 |
| fitting mathematical models to lactation curves from holstein cows in the southwestern region of the state of parana, brazil. | the objective of this study was to evaluate four mathematical models with regards to their fit to lactation curves of holstein cows from herds raised in the southwestern region of the state of parana, brazil. initially, 42,281 milk production records from 2005 to 2011 were obtained from "associação paranaense de criadores de bovinos da raça holandesa (apcbrh)". data lacking dates of drying and total milk production at 305 days of lactation were excluded, resulting in a remaining 15,142 records c ... | 2015 | 25806994 |
| molecular detection and occurrence of 'candidatus mycoplasma haemobos' in dairy cattle of southern brazil. | bovine hemoplasmas are bacteria found on the erythrocyte surface or free in the plasma of cattle. the aim of the present study was to evaluate the occurrence of 'candidatus mycoplasma haemobos' ('c. m. haemobos') in holstein and jersey cattle raised in londrina and surroundings, northern region of the state of parana, southern brazil. pcr testing directed to 16s rrna gene fragment was performed to investigate the occurrence and characterize the molecular identity of 'c. m. haemobos'. a total of ... | 2012 | 23070456 |
| spontaneous poisoning by hovenia dulcis in dairy cattle in southwest parana, brazil. | livestock poisoning by plants is a frequent occurrence which determines severe losses, such as the fall in the milk and meat production, the cost of expensive treatments, the state of immunosuppression, or even the animal's death. cattle ingest toxic plants only when there is food shortage, when they cannot select what they eat, or when they ingest food for preference, which is the case of hovenia dulcis fruits, very rich in sucrose. this plant is widely distributed in the southern and southeast ... | 2016 | 26415955 |