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incidence of arbovirus antibodies in bovine, ovine and human sera collected in eastern sicily.the incidence of antibodies against 9 arboviruses in ovine, bovine and human sera collected in eastern sicily was studied. a high incidence of antibodies was found especially against bhanja virus in all sera and against sandfly fever sicilian in ovine sera only. the incidence was higher than that reported in 1971 for the western part of sicily. these findings suggest an increase in the circulation of arboviruses in this area; the causes are briefly discussed.19767946
[the present-day clinico-epidemiological status of dermotyphous rickettsiosis]. 1977578006
[yersinia enterocolitica in fresh and packaged meats].in these last years it was observed an increased frequency of human infections by y. enterocolitica supported mostly in europe by serotype 0:3 and 0:9, and by serotype 0:8 in u.s.a. as far as it is concerned the transmission form to man, in addition to the possible infection between men, the one from animal to man and the one consequent on water use, most important suspicions are about foods derived from infected animals or however contaminated by man (fresh or preserved or processed meat, milk ...19892484479
molecular relationship among salmonella dublin isolates identified at the center for enterobacteriaceae of palermo during the years 1971-85.a molecular epidemiological study was carried out on 60 salmonella dublin isolates identified at the southern italy enterobacteriaceae center between 1971 and 1985. these included 23 isolates from children with diarrhoea in palermo obtained during 1984. all isolates from the outbreak of gastroenteritis in children were resistant to chloramphenicol and streptomycin and harboured two plasmids of 50 mda and 3 mda molecular weight, whereas the majority of the isolates identified before 1984 were sus ...19873678393
investigation on arboviruses in western sicily: insect collection and virus isolation. 19714403373
isolation of thogoto virus from rhipicephalus bursa ticks in western sicily. 19724680821
[research on the epidemiology-epizootology of leptospirosis. i. serological studies on the role of the hedgehog, erinaceus europaeus var. italicus barrett-hamilton, 1900, in the spread of pathogenic leptospira]. 19734807679
[bovine theileriasis in sicily due to th. annulata]. 19715152931
survey for arbovirus antibodies in domestic animals of western sicily. 19715153402
[on the microbial content of fresh beef in the market of messina]. 19666011734
haemagglutination inhibition antibodies against sindbis virus in human and bovine sera in eastern sicily. 19806107029
[breast feeding in palermo. preliminary results in a sample of 320 infants]. 19827155106
status of malaria vectors in italy.about 30 yr after malaria eradication, surveys to assess the presence and abundance of anopheline vectors were carried out in central and southern italy and in the islands of sardinia and sicily from 1992 to 1994. anopheles labranchiae falleroni was present in scattered foci in all regions, except for tuscany, where it breeds almost exclusively in rice fields (grosseto province). most common breeding sites were rivers and streams, followed by ponds and ground pools. the highest adult density was ...19979151488
assessment of the dairy production needs of cattle owners in southeastern sicily.this study was undertaken to investigate research and outreach priorities for progetto ibleo (project ibleo), a center created in 1990 with tripartite government funding to serve dairy producers in the hyblean region of sicily. data comprised values for production and composition of milk from 1984 to 1989 from 35 herds of modicana cows on a system based on pasture and that from 69 input-intensive herds of holstein cows, associated lactation and reproduction measures, and yield and composition of ...19989785243
livestock production and animal health in sicily, italy.in sicily, as in other mediterranean areas, livestock represents one of the most important resources for the island economy. this sector involves more than 16,000 farms of cattle and 10,000 farms of sheep and goats (respectively 6% and 15% of national production) which are actually increasing their number. most livestock in sicily is owned by small holders and pastoralists. regional production of milk feeds some industries, which involve a large and increasing occupational area. due to its pecul ...199911071536
bovine babesiosis in sicily: preliminary study on pathology.in this study, the authors report the preliminary pathological features of an outbreak of spontaneous babesiosis detected in a group of thirty-five, five to six months-old simmenthal calves imported in sicily in november 1998. five of them showed fever, anaemia, haemoglobinuria, inappetence, depression, thirst, swollen lymph-nodes and poor body conditions a few days after being introduced in the farm. several ticks were present particularly at the basis of the horns and near the ears of the sick ...199911071540
clinical and laboratory studies on theileriosis outbreaks in sicily, italy.a total of 153 blood samples were collected all over the sicilian island in 15 farms. 29.4% and 25.5% were pcr positive for theileria annulata and t. buffeli/orientalis respectively. 15.7% of cattle blood samples showed a mixed infection with both theileria species. each farm showed an infection rate ranging from 0% to 100%. hyalomma marginatum and boophilus annulatus were the most common ticks collected in april and november 1998 respectively. the aim of the present work was to investigate on h ...199911071547
characterization of theileria species by pcr using specific target sequences.theileriosis is an infectious disease in tropical countries and in the mediterranean area. it is caused by theileria, a haemoprotozoan, transmitted by vectors belonging to the ixodidae. in southern italy and in sicily the infection is due mainly to t. annulata, but in some cases other species are involved in the disease. the authors describe a method to identify theileriosis in cattle blood samples, using pcr and hybridization techniques. different primer sets were used to amplify different dna ...199911071548
integrated molecular diagnosis of theileria and babesia species of cattle in italy.a reverse line blot hybridization (rlb) test was developed to specifically identify six theileria spp. (t. annulata, t. parva, t. mutans, t. velifera, t. taurotragi, and t. buffeli/orientalis) and three babesia spp. (b. bovis, b. bigemina, and b. divergens). no cross reaction was observed with other livestock pathogens (such as anaplasma marginale, a. centrale, a. ovis, cowdria ruminantium, trypanosoma brucei, t. congolense, and t. vivax). this method was used to test bovine blood samples collec ...200011193668
mass spectrometry for cultural heritage knowledge: gas chromatographic/mass spectrometric analysis of organic remains in neolithic potsherds. 200111333449
[the risk of escherichia coli o157:h7 in sicily, through imported bovines for slaughter]. 200111414107
detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in sicily.a reverse line blot hybridisation (rlb) of 21 oligonucleotides with polymerase chain reaction (pcr) amplified regions of 16s rrna (ehrlichia/anaplasma group) or 18s rrna (babesia/theileria group) genes of haemoparasites detected theileria annulata, t. buffeli/orientalis, babesia bovis, b. bigemina, b. divergens, ehrlichia bovis, anaplasma marginale, a. centrale and unknown species within the rickettsia tribe.a very high prevalence of mixed infections was detected, which indicated that animals in ...200111511414
characterization of shiga toxin-producing escherichia coli o157:h7 isolated in italy and in france.twenty-one escherichia coli o157:h7 strains isolated in northern italy from sporadic cases of hemolytic-uremic syndrome and from cattle and food were characterized by virulence gene analysis, pulsed-field gel electrophoresis (pfge) of xbai-digested dna, enterobacterial repetitive intergenic consensus (eric) sequence-based pcr (eric-pcr), and antibiotic resistance patterns and compared to 18 strains isolated in france from human cases of diarrhea, cattle, and the environment. strains isolated in ...200212454162
contribution of native pasture to the sensory properties of ragusano cheese.ragusano is a protected denomination of origin cheese produced in the hyblean area of sicily. sixteen samples of ragusano cheese from two different treatments [pasture and total mixed ration (tmr)] were evaluated after 4 and 7 mo of aging. the color of the cheeses produced from milk of cows consuming fresh native pasture plants was much more yellow than cheeses from tmr fed cows (i.e., higher hunter b value). this was due to transfer of beta-carotene and related compounds from the diet and demon ...200414762073
spatial distribution of bluetongue virus and its culicoides vectors in sicily.during the recent mediterranean epizootic of bluetongue, an extensive programme of serological and vector (culicoides biting midges (diptera: ceratopogonidae)) surveillance was carried out across sicily. this paper presents the analysis of 911 light trap catches collected at the times of peak culicoides abundance (summer to autumn 2000-2002) in 269 sites, in order to produce detailed maps of the spatial distribution of the main european vector, culicoides imicola kieffer and that of potential no ...200415189232
modelling the distributions of culicoides bluetongue virus vectors in sicily in relation to satellite-derived climate variables.surveillance data from 268 sites in sicily are used to develop climatic models for prediction of the distribution of the main european bluetongue virus (btv) vector culicoides imicola kieffer (diptera: ceratopogonidae) and of potential novel vectors, culicoides pulicaris linnaeus, culicoides obsoletus group meigen and culicoides newsteadi austen. the models containing the 'best' climatic predictors of distribution for each species, were selected from combinations of 40 temporally fourier-process ...200415189233
composition and aroma compounds of ragusano cheese: native pasture and total mixed rations.raw milk from 13 cows fed tmr supplemented with native pasture and from 13 cows fed only tmr on one farm was collected separately 4 times with an interval of 15 d between collections. two blocks (14 kg each) of cheese were made from each milk. the objective was to determine the influence of consumption of native plants in sicilian pastures on the aroma compounds present in ragusano cheese. milk from cows that consumed native pasture plants produced cheeses with more odor-active compounds. in 4-m ...200415259216
study of gastrointestinal nematodes in sicilian sheep and goats.parasitic gastroenteritis is one of the major causes of productivity loss in sheep and goats. this report records two studies of the helminth fauna from post-mortem examination. the first study, performed on the digestive tract of 72 sheep from a central part of sicily in a high hill village (1,360 meters above sea level), between april 1996 and march 1997, showed an infection rate of 78%. the second study targeted goats from the western part of sicily and showed an infection rate of 90%. for sh ...200415604491
[angelo maria maffucci (1845-1903) and the beginning of pathological anatomy in pisa].in 1884, at the faculty of medicine of the university of pisa, the new chair of pathology was inaugurated and angelo maria maffucci was appointed first director. angelo maffucci was born in 1845 in calitri, near avellino, from a farming family. after graduating in medicine at naples, in 1872, he started his scientific work at the institute of pathology, under otto von schrön (1837-1917). he was professor of general pathology in messina then professor of pathology at the university of catania. in ...200415685797
[occupational brucellosis in slaughtering of sheep and goats: study of five cases from a municipal abattoir in south-eastern sicily].brucellosis is a world-wild zoonosis of bacterial origin. in italy, where reporting of the disease is mandatory, the incidence is 2.1 cases every 100,000 inhabitants, but tends to be higher in southern regions. however, the incidence decreased from 1999 to 2002.200516001513
serologic and molecular characterization of anaplasma species infection in farm animals and ticks from sicily.although anaplasma marginale was known to be endemic in italy, the diversity of anaplasma spp. from this area have not been characterized. in this study, the prevalence of anaplasma spp. antibodies in randomly selected farm animals collected on the island of sicily was determined by use of a msp5 celisa for anaplasma spp. and an immunofluorescence test specific for anaplasma phagocytophilum. genetic variation among strains of anaplasma spp. from animals and ticks was characterized using the a. m ...200516043300
cystic echinococcosis in italy from the 1950s to present.in italy the epidemiological pattern of cistic echinococcosis (ce) is incomplete and the information for most regions is out of date, contradictory, and almost exclusively limited to the intermediate hosts. the disease is found most frequently in particular social and economic conditions: widespread use of extensive or semi-extensive sheep farming, illegal slaughtering, and high numbers of sheepdogs and other types of dogs. the highest incidence in sheep is found in sardinia (70.6-92.8%), sicily ...200416044697
an epidemiological updating on cystic echinococcosis in cattle and sheep in sicily, italy. 200416044707
genetic diversity of anaplasma marginale strains from cattle farms in the province of palermo, sicily.bovine anaplasmosis, caused by the tick-borne rickettsia anaplasma marginale, is endemic in sicily and results in economic loss to the cattle industry. this study was designed to characterize strains of a. marginale at the molecular level from cattle in the province of palermo, sicily. seropositivity of cattle >or=1 year old for a. marginale in the study area ranged from 62% to 100%. the observed prevalence of a. marginale infections in cattle herds ranged from 25% to 100%. two predominant a. ma ...200516115096
ticks infesting livestock on farms in western sicily, italy.the results of a 2-year survey on ticks infesting livestock on farms in western sicily, italy, are reported. several dogs living in the same area were also examined for ticks. moreover, free-living ticks from one cattle farm were caught by co(2) traps and flagging. a total of 6208 specimens was collected belonging to 9 species: rhipicephalus bursa (32.4%), rhipicephalus turanicus (22.7%), rhipicephalus sanguineus (19.3%), hyalomma lusitanicum (12.0%), haemaphysalis punctata (7.8%), hyalomma marg ...200616550337
esat-6 peptide recognition by bovine cd8+ lymphocytes of naturally infected cows in herds from southern italy.the aim of this study was to define epitopes of mycobacterium bovis from esat-6 (early secretory antigen of 6 kda) recognized by cd8+ t lymphocytes from cows naturally infected with mycobacterium bovis. we found that bovine cd8+ t cells recognized 10 out of 11 esat-6 peptides tested.200616603624
molecular characterization of anaplasma platys strains from dogs in sicily, italy.the genetic diversity of anaplasma platys (rickettsiales: anaplasmataceae) strains is currently poorly defined. the present study was designed to characterize a. patys strains in dogs from palermo, sicily, italy, using a combination of pcr and sequence analysis of the 16s rdna, heat shock operon groesl and citrate synthase (glta) genes.200616872489
updates on cystic echinococcosis (ce) in italy.an update on cystic echinococcosis (ce) diffusion in italy during 2003-2005 is reported. ce seems to have a sporadic diffusion in the northern part of the country where this disease plays a minor role (prevalence < 1%). recent investigations have shown the occurrence of ce cases in humans from the mountains between reggio emilia and modena, with an average year incidence between 9.4 and 5.6/100,000. in abruzzo prevalences in sheep and cattle are 20.2% and 15.3%, with a fertility of 4.6% and 1.3% ...200616881397
molecular epidemiology of human and bovine anaplasmosis in southern europe.the genus anaplasma (rickettsiales: anaplasmataceae) includes several pathogens such as a. marginale and a. phagocytophilum that have an impact on veterinary and human health. in this study, we characterized a. marginale and a. phagocytophilum infections in humans, wild and domestic animals, and ticks in southern europe (particularly in south-central spain and in sicily) by means of serologic study, pcr, and sequence analysis of major surface proteins (msp) 1alpha and 4 and 16s rdna. the results ...200617114686
antioxidant activity of sicilian pistachio (pistacia vera l. var. bronte) nut extract and its bioactive components.pistacia vera l. is the only species of pistacia genus producing edible nuts. this paper investigates the antioxidant potential of a sicilian variety of pistachio nut by chemical as well as biological assays and measured antioxidant vitamins and a number of antioxidant polyphenols in either the hydrophilic and/or the lipophilic nut extract. in accordance with the majority of foods, the total antioxidant activity, measured as a taa test, was much higher (50-fold) in the hydrophilic than in the li ...200717263455
observed prevalence of tick-borne pathogens in domestic animals in sicily, italy during 2003-2005.the objective of this study was to characterize the observed prevalence of tick-borne pathogens (tbp) in domestic animals in sicily, italy during 2003-2005. serological (competitive elisa and indirect immunofluorescence antibody, n = 3299) and dna tests (polymerase chain reaction and reverse line blot, n = 2565) were conducted on horse, donkey, cattle, sheep, goat, pig and dog samples. pathogens analysed included anaplasma, ehrlichia, rickettsia, babesia and theileria species, and coxiella burne ...200717359441
anaplasmosis in cattle in italy.bovine anaplasmosis caused by anaplasma marginale is a disease transmitted by ticks belonging to the ixodidae family. southern italy is considered an endemic zone but environmental and social factors are changing the epidemiology of the disease to expand to previously anaplasmosis-free regions. the available data of published reports of anaplasmosis in italy together with the data obtained by the national centre of reference for anaplasma, babesia, rickettsia and theileria (c.r.a.ba.r.t.), allow ...200717682850
polymerase chain reaction method for leptospirosis, analysis on samples from an autochthon swine population in sicily, italy.we set a method targeting 16 rrna gene consisting in a single polymerase chain reaction of 40 cycles which is specific for pathogenic leptospira. negative polymerase chain reaction results were observed with nonpathogenic leptospira (serovar patoc) and other bacteria species. by this method a survey on a population of autochthon swine herds had been conducted in sicily particularly on kidney samples of slaughtered animals and on urine samples from live animals. the analysis showed that a prevale ...200517966471
prevalence of mastitis pathogens in ragusa, sicily, from 2000 to 2006.the objective of this study was to report the prevalence of intramammary infections (imi) in ragusa, sicily, from milk samples (n = 18,711) collected between october 2000 and june 2006 from 101 dairy herds. milk samples were collected at 9,747 cow sampling events from 5,285 individual cows. samples were individual quarter (92.8%) or composite samples (7.2%) from an individual cow. logistic regression was used to examine the prevalence of imi at the level of milk sample and at the level of cow, c ...200718024774
prevalence and genotypes of anaplasma species and habitat suitability for ticks in a mediterranean ecosystem.anaplasma species are tick-transmitted pathogens that impact veterinary and human health. sicily is one of the locations where these pathogens are endemic. sicily represents a typical mediterranean ecosystem to study anaplasma infection and tick habitat suitability. the aims of this study were (i) to characterize by 16s rrna and species-specific msp4 gene pcr the prevalence and genotypes of a. marginale, a. phagocytophilum, and a. ovis in the most abundant host species in sicilian provinces and ...200818978093
characterization of anaplasma infections in sicily, italy.this study aimed to characterize infection with anaplasma marginale, a. phagocytophilum, a. ovis, and a. platys in humans, animals, and ticks in sicily, italy, during 2003-2006. serologic (competitive elisa [celisa]) and indirect immunofluorescence antibody [ifa]; n= 1990) and dna (polymerase chain reaction [pcr]; n= 2788) tests were conducted on horse, donkey, cattle, sheep, goat, pig, dog, cat, roe deer, wild boar, human, and tick samples. the results reported herein suggested that in sicily c ...200819120181
prevalence of anaplasma species and habitat suitability for ticks in sicily. 200919416281
ovine catarrhal fever (bluetongue): analysis of culicoides species in seropositive farms.bluetongue (bt) is an orbiviral disease of wild and domestic ruminants, mainly sheep. in sicily, the first bluetongue outbreak occurred in october 2000; there have been 76 recorded outbreaks so far. the national surveillance plan, based on european union commission decision 138/2001/ce, establishes serological and entomological surveys. this plan consists of controls of seronegative cattle, called 'sentry' as indicators for the presence and circulation of virus in defined areas. to check the ser ...201020537094
prevalence of tick-borne pathogens in ticks in sicily.the prevalence of anaplasma, ehrlichia, rickettsia and babesia/theileria species was analysed in questing and feeding adult ticks in sicily. a total of 678 ticks were collected and analysed in this study. of these, 29 were questing ticks and 649 were collected from infested cattle, sheep, goats or dogs. tick species analysed included rhipicephalus bursa, r. turanicus, r. sanguineus, hyalomma lusitanicum, h. marginatum, dermacentor marginatus, ixodes ricinus, r. (boophilus) annulatus and haemaphy ...201020537102
[the return of anthrax. from bioterrorism to the zoonotic cluster of sciacca district].anthrax is a disease caused by bacillus anthracis which affects herbivorous animals. humans acquire the disease incidentally by exposure to infected animals, animal products or spores on soil. the infection is still endemic in many regions in developing countries. in italy animal clusters are very rare and human cases are exceptional. bacillus anthrax is also a potential source for acts of bioterrorism. in the natural human infection, cutaneous anthrax is the most widespread, while the other two ...201020610930
associations of breed and feeding management with milk production curves at herd level using a random regression test-day model.earlier studies identified large between-herd variation in estimated lactation curve parameters from test-day milk yield and milk composition records collected in ragusa province, italy. the objective of this study was to identify sources of variation able to explain these between-herd differences in milk production curves, by estimating associations of animal breed (holstein friesian vs. brown swiss), feeding system [separate feeding (sf) vs. total mixed ration (tmr)], and tmr chemical composit ...201020855033
molecular characterization of genotype g6 human rotavirus strains detected in italy from 1986 to 2009.group a human rotavirus (hrv) strains with a bovine-like (g6) major outer capsid protein vp7 were first detected in palermo, italy, in the late 1980s, and subsequently worldwide. during a 25-year rotavirus surveillance period, additional hrv g6 strains, associated with either a p[9] or p[14] vp4 genotype, have been detected sporadically, but repeatedly, in palermo. whether these g6 hrvs were transmitted to humans directly from an animal reservoir or could have circulated at low prevalence in sus ...201121640847
Characterization of street food consumption in palermo: possible effects on health.ABSTRACT:201122034903
effect of farming system and cheesemaking technology on the physicochemical characteristics, fatty acid profile, and sensory properties of caciocavallo palermitano cheese.caciocavallo palermitano is a typical stretched-curd cheese that has been produced over the centuries in sicily according to traditional cheesemaking technology and using raw milk from autochthonous cow breeds reared at pasture. the objective of this experiment was to evaluate the effects of the farming system and processing technology on the characteristics of caciocavallo palermitano cheese, with particular regard to the fatty acid profile. the farming system was either extensive, using autoch ...201323127907
preselection statistics and random forest classification identify population informative single nucleotide polymorphisms in cosmopolitan and autochthonous cattle breeds.commercial single nucleotide polymorphism (snp) arrays have been recently developed for several species and can be used to identify informative markers to differentiate breeds or populations for several downstream applications. to identify the most discriminating genetic markers among thousands of genotyped snps, a few statistical approaches have been proposed. in this work, we compared several methods of snps preselection (delta, f st and principal component analyses (pca)) in addition to rando ...201828643617
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