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seroprevalence of borna disease virus in domestic animals in xinjiang, china.to investigate the animals infected with borna disease virus (bdv) in xinjiang, china, we examined for bdv antibodies in the sera from groups of 20 horses, sheep and cattle, and from 165 wild rodents (18 species) by elisa and immunoblot. the serological study disclosed the presence of antibodies to both bdv-p24 and -p40 in the horses (20%) and sheep (25%), whereas no apparent positive reaction was detected either in cattle or rodents. the results suggested that bdv is prevalent in horses and she ...200111348775
when and how did bos indicus introgress into mongolian cattle?the mongolian cattle are one of the most widespread breeds with strictly bos taurus morphological features in northern china. in our current study, we presented a diversity of mitochondrial dna (mtdna) d-loop region and y chromosome snp markers in 25 male and 8 female samples of mongolian cattle from the xinjiang uygur autonomous region in western china, and detected 21 b. taurus and four bos indicus (zebu) mtdna haplotypes. among four b. indicus mtdna haplotypes, two haplotypes belonged to i1 h ...201424418696
genetic diversity of streptococcus uberis isolates from dairy cows with subclinical mastitis in southern xinjiang province, china.streptococcus uberis is a common cause of dairy cow mastitis throughout the world. the failure to control bovine mastitis caused by s. uberis is largely attributed to the little known about the epidemiology of this bacteria, especially strain differences in the same area. to define the local epidemiology of s. uberis in the south of xinjiang, china, we explored the genetic diversity of 28 bovine subclinical mastitis field isolates of s. uberis, collected from 3 chinese farms during 2009 and 2010 ...201324005178
evaluation of spoligotyping and miru-vntr for mycobacterium bovis in xinjiang, china.mycobacterium bovis, the causative agent of bovine tuberculosis (btb) in cattle, is also a pathogen for human and other mammals. in this study, 406 cows were screened for btb by both single intradermal comparative cervical tuberculin (sicct) test and ifn-γ assay. 135 m. bovis were isolated from 31 sicct and ifn-γ double-positive cows in xinjiang uygur autonomous region of china. spoligotyping and miru-vntr were evaluated for genotyping, and 4 and 7 genotypes were identified, respectively. a new ...201121507443
genetic typing and epidemiologic observation of bovine viral diarrhea virus in western china.whole blood samples (472) were collected during the period of 2006-2008 from 15 cattle farms in seven districts of the xinjiang uygur autonomous region, which is a major pastoral area and represents 1/6 of chinese territory. bovine viral diarrhea virus (bvdv) was detected by nested reverse transcription polymerase chain reaction assay (rt-npcr) and sequenced within the 5'-untranslated region of the genome. approximately 43% (202 samples) were bvdv-positive. of the positive samples, 24 were chose ...201121132458
[detection and sequences analysis of bovine hepatitis e virus rna in xinjiang autonomous region].reverse transcription-nested polymerase chain reaction (rt-npcr) was used to detect hev (hepatitis e virus, hev) rna in dung or anus-swab samples collected from the cows with the positive anti-hev antibodies in dairy farms in xinjiang autonomous region. 7 of 60 (11.67%) cows were positive for hev rna in one farm. 1 of 31 (3.23%) cows was positive in the other farm. pcr amplification products were cloned, sequenced and analyzed. the result showed that the homology among the 8 bovine hev orf2 189b ...201020329555
detection of dna closely related to 'candidatus rickettsia principis' in haemaphysalis danieli recovered from cattle in xinjiang uygur autonomous region area, china.tick dna samples from cattle in xinjiang uygur autonomous region area, china, were examined for rickettsia infection by citrate synthase gene-based pcr and sequencing. four positive samples were detected from haemaphysalis danieli and high levels of similarity were found with recently detected 'candidatus rickettsia principis.'200717052853
sero-epidemiological surveillance of hypodermosis in yaks and cattle in north china by elisa.a large-scale serological surveillance was conducted for hypodermosis in the north of china. a total of 4175 sera samples of yaks and cattle were collected from five provinces in north china that included xinjiang, inner mongolia, heilongjiang, jiling and gansu provinces, between 2001 and 2002, and were examined for anti-hypoderma antibody by elisa. the results indicate that the naturally infested positive rates of hypoderma spp. in the above provinces were 51.77%, 27.02%, 13.00%, 6.03% and 44.4 ...200515817213
detection of immunoglobulin g to crimean-congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays.crimean-congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. the recombinant nucleoprotein-based crimean-congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (elisa) and an indirect immunofluorescence assay techniques. the samples used for evaluation were 80 sera co ...200312565161
mitochondrial genomic markers confirm the presence of the camel strain (g6 genotype) of echinococcus granulosus in north-western china.twenty-eight isolates of e. granulosus, collected from humans at surgery, and a range of intermediate hosts, including sheep, cattle and camels from abattoirs in north and south xinjiang uygur autonomous region, people's republic of china, were analysed for dna sequence variation within regions of the mitochondrial cytochrome c oxidase i (coi) and nadh dehydrogenase subunit i (ndi) genes. the isolates were categorized into 2 distinct and uniform genotypic groupings, based on the sequences obtain ...19989481771
cystic echinococcosis in the xinjiang/uygur autonomous region, people's republic of china. i. demographic and epidemiologic data.demographic and epidemiologic data on those factors potentially related to the prevalence and transmission of hydatid disease caused by echinococcus granulosus were collected in 1987 from each household in 85 villages in the two communities of yuanhucun and ershilidian within hutubi county in the xinjiang/uygur autonomous region, prc, with additional questionnaire data collected from approximately 40 households randomly selected from each of 16 villages within those communities. yuanhucun and er ...19902382094
prevalence and molecular characterization of cryptosporidium spp. and giardia duodenalis in dairy cattle in beijing, china.822 fecal samples from cattle in six areas of beijing were examined with microscopy for cryptosporidium oocysts and giardia cysts. the overall infection rates for cryptosporidium spp. and giardia duodenalis were 2.55% and 1.09%, respectively. cryptosporidium was only detected in calves and heifers, whereas g. duodenalis was found in all age groups. cryptosporidium spp. were characterized with a pcr-restriction fragment length polymorphism analysis and dna sequence analysis of the small subunit ( ...201626921041
prevalence and multilocus genotyping of cryptosporidium andersoni in dairy cattle and he cattle in xinjiang, china.cryptosporidium andersoni is the predominant species in post-weaned and adult cattle in china. the aim of this study was to determine the prevalence and understand the transmission of cattle cryptosporidiosis in the xinjiang uyghur autonomous region, china, a total of 1827 fecal samples (436 from he cattle and 1391 from dairy cattle) were examined for the presence of c. andersoni-like oocysts by microscopy after sheather's sugar flotation technique. the overall prevalence of c. andersoni-like wa ...201627448954
[detection and sequences analysis of sheep hepatitis e virus rna in xinjiang autonomous region].to understand whether hepatitis e virus (hev) was infectious in sheep in xinjiang.201020815242
outbreak of trichophyton verrucosum in china transmitted from cows to humans.twenty percent of 1000 dairy cows were infected with trichophyton verrucosum in a newly established dairy farm in western china. the disease was transmitted to 30 of 100 animal workers. the source of the outbreak is unknown but it is suspected that naïve cattle which were transported from holland to china became infected after they arrived in xinjiang province (west china) where the fungus is endemic in nature, prepuberty school children and various livestock.200616552485
description of a new babesia sp. infective for cattle in china.a babesia species has been identified and shown to be transmitted by hyalomma anatolicum anatolicum in china. when larvae, nymphs and adults developed from engorged females h. a. anatolicum collected from cattle in xinjiang province were infested onto the babesia-free calves, piroplasms of babesia sp. were seen in blood smears from cattle infested with nymphs (2 calves), but not from the calves infested with larvae (1 calf or adult ticks (2 calves). this babesia sp. proved to be of low virulence ...200212051599
research on the schizont cell culture vaccine against theileria annulata infection in xinjiang, china.theileria annulata infection (tai) is one of the most serious diseases of cattle in xinjiang autonomous region of uigur minority nationality. it has been recorded in 14 prefectures, except the tulufan prefecture, but the enzootic areas are mainly distributed around the zhunger basin and talim basin. according to the records collected in the ten years before vaccination with the schizont cell culture vaccine was carried out, the average incidence rate of tai in enzootic areas was 7.22% and the mo ...19979512753
characterization and complete genome sequence analysis of a novel virulent siphoviridae phage against staphylococcus aureus isolated from bovine mastitis in xinjiang, china.bovine mastitis is one of the most costly diseases in dairy cows worldwide. it can be caused by over 150 different microorganisms, where staphylococcus aureus is the most frequently isolated and a major pathogen responsible for heavy economic losses in dairy industry. although antibiotic therapy is most widely used, alternative treatments are necessary due to the increasing antibiotic resistance. using phage for pathogen control is a promising tool in the fight against antibiotic resistance. mai ...201728299517
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