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partial purification of hepatitis b surface antigen and survey of this antigen in man and animals in chiang mai, thailand. 1978311951
antigenic and genetic analyses of human rotaviruses in chiang mai, thailand: evidence for a close relationship between human and animal rotaviruses.serotyping of group a rotaviruses obtained from stools of 158 diarrheic patients in chiang mai, thailand, by elisa with monoclonal antibodies revealed a yearly change in the prevalence of individual serotypes. three unusual human rotavirus strains were isolated. strain mc35 had subgroup i-serotype 10 antigen and a long rna electrophoretic type, a property hitherto found only in bovine rotaviruses. rna-rna hybridization tests showed that the strain is more closely related genetically to bovine th ...19921378870
seroprevalence of melioidosis in dairy cattle in chiang mai province, northern thailand.the seroprevalence of melioidosis in dairy cattle in chiang mai province was investigated using of the indirect hemagglutination antibody (iha) method. two hundred and fifty-three samples were tested for serum antibodies to burkholderia pseudomallei. the samples were from a total population of 8,688 dairy cattle in the province; random sampling, stratified by the location of cattle, was used. the seroprevalence was determined as 2% at 1:40 cut-off value, which was estimated to equate to 0.3% to ...200212757220
campylobacter in food animals and humans in northern thailand.cross-sectional, longitudinal, and case-control studies were conducted to describe the epidemiology of campylobacter in chickens, swine, dairy cows, farm workers, nonfarm residents, and children with diarrhea. samples were collected in chiang mai and lamphung provinces of northern thailand from 2000 through 2003. a total of 2,360 samples were processed. results from the cross-sectional study indicated that the prevalences of campylobacter in chickens at the farm, slaughterhouse, and market were ...200516355821
high annealing temperature-random amplified polymorphic dna (hat-rapd) analysis of three paramphistome flukes from thailand.the rumen flukes of 37 cows (bos indicus) from chiang mai and lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). three species were found: paramphistomum epiclitum, orthocoelium streptocoelium, and fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. genomic dna was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic dna (hat-rapd) technique. ...200716889772
multiple combinations of p[13]-like genotype with g3, g4, and g5 in porcine rotaviruses.epidemiological surveillance of porcine rotavirus (porv) strains was carried out in chiang mai province, thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by pcr. of these, six were g3 and one was g4 and displayed a p-nontypeable genotype, while another isolate was both g and p nontypeable. analysis of a partial vp4 gene of all eight p-nontypeable strains revealed a high degree of amino acid sequence identities (94.7% to 100%), suggesting that they belonged ...200818234859
genetic characterization of group c rotavirus isolated from a child hospitalized with acute gastroenteritis in chiang mai, thailand.during an epidemiological survey of human rotavirus infection in chiang mai, thailand, from 2002 to 2004, in which 263 stool specimens tested, one isolate of group c rotavirus was detected from a two-year-old child admitted to hospital with acute gastroenteritis. the human group c rotavirus, named cmh004/03, was characterized further by molecular analyses of its vp4, vp6, and vp7 gene segments as well as determination of rna pattern by polyacrylamide gel electrophoresis (page). molecular charact ...200818696224
seroprevalence of cryptosporidium parvum infection of dairy cows in three northern provinces of thailand determined by enzyme-linked immunosorbent assay using recombinant antigen cpp23.cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in aids patients in thailand. cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. a 23-kda glycoprotein of c. parvum (cpp23) is a sporozoite surface protein that is geographically conserved among c. parvum isolates. this glycoprotein is a potentially useful candidate antigen for the diagnosis of cryptosporidiosis by enzyme-linked immunosorbe ...200920698436
seroprevalence of neospora caninum infection in dairy cows in northern provinces, thailand.neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of neosporosis, recognized as a major cause of bovine abortion around the world. thailand is a developing agricultural country located in southeast asia. livestock developments particularly in dairy cows of this country have been hampered by low productivity including milk and slow growth rate due to the impact of many pathogens including n. caninum. currently, there is no effective method for control of neosp ...201424827103
quarter, cow, and farm risk factors for intramammary infections with major pathogens relative to minor pathogens in thai dairy cows.a cross-sectional study was carried out from may to september 2011 on 35 smallholder dairy farms in chiang mai, thailand, to identify the quarter, cow, and farm factors that relate to intramammary infections (imi) from major specified pathogens, compared to infections from minor pathogens. data on general farm management, milking management, and dry cow management were recorded for each herd. quarter milk samples were collected from either clinical or subclinical mastitis quarters. dependent var ...201424823898
prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, thailand.to investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region.201424507641
seroprevalence of babesia infections of dairy cows in northern thailand.the present study was conducted to demonstrate the epidemiological distribution of bovine babesiosis in the northern regions of thailand. a total of 700 serum samples of dairy cows in the northern provinces (chiang rai, chiang mai, lumpang, and mae hong sorn) were tested for antibodies against babesia bovis and b. bigemina. species-specific enzyme-linked (rrap-1/cts) were performed. according to the results, 517 (73.8%) and 484 (69.1%) were positive for b. bovis and b. bigemina, respectively. in ...201020363078
highland-lowland conflict over natural resources: a case of mae soi, chiang mai, thailand.interviews were conducted among 150 hmong highland and ethnic thai lowland villagers during may-august 1992 in chiang mai in northern thailand. officials from national and international drug abuse control and development agencies were also interviewed. the aim was to determine the nature of the conflict between the highlanders and the lowlanders. the lowlanders perceived that the conflict was due to environmental causes brought on by the hmongs' destruction of forest and pollution of the rive ...199512347196
identification of virulence-associated antigens and plasmids in rhodococcus equi from patients with acquired immune deficiency syndrome and prevalence of virulent r. equi in soil collected from domestic animal farms in chiang mai, thailand.the prevalence of virulent rhodococcus equi in soil collected from 17 domestic animal farms (from 12 cattle, 1 pig, and 4 horse farms) and in 6 clinical specimens from patients with acquired immune deficiency syndrome (aids) in chiang mai, thailand, was investigated. the isolates were tested for the presence of 15-17-kda antigens (vapa) and a 20-kda antigen (vapb) by immunoblotting and for the presence of virulence plasmid dna. rhodococcus equi was isolated from most soil samples (68 of 80) obta ...200212135268
heterogeneity in the host preference of japanese encephalitis vectors in chiang mai, northern thailand.experiments, using the capture-mark-release-recapture technique inside large nets, were carried out in chiang mai, northern thailand, to examine heterogeneity in the host preference of japanese encephalitis (je) vectors. a significantly higher proportion of the vector species that were initially attracted to a cow fed when released into a net with a cow than when released into a net containing a pig. however, culex vishnui individuals that had been attracted to a pig had a higher feeding rate in ...200010974986
experimental infection of thailand taenia (chiengmai strain) in domestic animals.twenty-five gravid proglottides of a thailand taenia were obtained from a patient in chiengmai, thailand, and brought to our laboratory. the tapeworm was determined to be t. saginata-like by counting uterine branches (mean number 16, range 12-19 on each side). the eggs from these proglottides remained infective under storage at room temperature for 14 days followed by refrigeration (4-8 degrees c) for 131 days. eight small-ear-miniature pigs and two holstein calves were each fed with 3000 eggs a ...19902312220
prevalence and molecular characterization of bovine cryptosporidium from dairy cows in northern thailand.cryptosporidiosis is a common protozoan infection in humans and domestic animals. it is the culprit for significant neonatal morbidity in cattle as well as weight loss and delayed growth, which leads to large economic losses in the farming industry. furthermore, bovine cryptosporidium is also a principal source of human cryptosporidium infections. the purpose of this study is to determine prevalence and genotype of cryptosporidium spp. from feces of dairy cows from the northern parts of thailand ...201729035847
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