| an insulin-like growth factor homologue of singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication. | insulin-like growth factors (igfs) play crucial roles in regulating cell differentiation, proliferation and apoptosis. in this study, a novel igf homologue gene (igf-like) encoded by singapore grouper iridovirus (sgiv) orf062r (termed sgiv-igf), was cloned and characterized. the coding region of sgiv-igf is 771 bp in length, with a variable number of tandem repeats (vntr) locus at the 3'-end. we cloned one isoform of this novel gene, 582 bp in length, containing the predicted igf domain and 3.6 ... | 2013 | 24062533 |
| characterization of the vp39 envelope protein from singapore grouper iridovirus. | singapore grouper iridovirus (sgiv) is a major pathogen that causes heavy economic losses to the grouper aquaculture industry in china and southeast asian countries. in the present study, a viral envelope protein, vp39, encoded by sgiv orf39l, was identified and characterized. sgiv orf39l was found in all sequenced iridoviruses and is now considered to be a core gene of the family iridoviridae. orf39l was classified as a late gene during in vitro infection using reverse transcription-polymerase ... | 2015 | 26524136 |
| itraq analysis of singapore grouper iridovirus infection in a grouper embryonic cell line. | we report, here, the first proteomics study of a grouper embryonic cell line (gec) infected by singapore grouper iridovirus (sgiv). the differential proteomes of gec with and without viral infection were studied and quantified with itraq labelling followed by liquid chromatography/tandem mass spectrometry (lc-ms/ms). forty-nine viral proteins were recognized, of which 11 were identified for the first time. moreover, 743 host proteins were revealed and classified into 218 unique protein groups. f ... | 2008 | 18931085 |
| identification and characterization of a putative lipopolysaccharide-induced tnf-alpha factor (litaf) homolog from singapore grouper iridovirus. | lipopolysaccharide-induced tnf-alpha factor (litaf), a transcription factor, can regulate tumor necrosis factor alpha (tnf-alpha) transcription. here, a novel litaf homolog encoded by singapore grouper iridovirus (sgiv litaf) was identified and characterized. the putative sgiv litaf encoded a protein of 104 amino acids (aa) with a predicted molecular mass of 11.6 kda. reverse transcription-pcr (rt-pcr) and western blot analyses of sgiv-infected cells revealed that sgiv litaf was an early viral g ... | 2008 | 18554501 |
| temporal and differential gene expression of singapore grouper iridovirus. | singapore grouper iridovirus (sgiv), an iridovirus in the genus ranavirus, is a major pathogen that results in significant economic losses in grouper aquaculture. to investigate further its infective mechanisms, for the first time, a viral dna microarray was generated for the sgiv genome to measure the expression of its predicted open reading frames simultaneously in vitro. by using the viral dna microarray, the temporal gene expression of sgiv was characterized and the dna microarray data were ... | 2006 | 16963749 |
| proteomic studies of the singapore grouper iridovirus. | the singapore grouper iridovirus (sgiv) genome consists of a double-stranded circular dna of 140,131 base pairs with 162 predicted open reading frames. our earlier study using peptide mass fingerprints generated from maldi-tof ms led to the identification of 26 viral proteins. the present investigation aimed to achieve a more comprehensive and precise identification of the sgiv viral proteome by two workflows: one-dimensional gel electrophoresis (1-de) separation followed by protein identificati ... | 2006 | 16263702 |
| in situ hybridization of a marine fish virus, singapore grouper iridovirus with a nucleic acid probe of major capsid protein. | a dna probe of 531 base pairs for singapore grouper iridovirus (sgiv) was generated by polymerase chain reaction and labeled with nonradioactive digoxigenin. an in situ hybridization based method was developed to detect sgiv in formalin-fixed tissues from maricultured malabar grouper, epinephelus malabaricus bloch and schneider. the in situ hybridization detected sgiv in the kidney, spleen, liver, intestine, stomach and gills from naturally infected fish. strong hybridization signals were obtain ... | 2004 | 15041208 |