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determination of bovine rotavirus g genotypes in kashmir, india.rotavirus ribonucleic acid (rna) was extracted from ten faecal samples of diarrhoeic calves positive for group a rotavirus by enzyme-linked immunosorbent assay (elisa). a portion of the extracted rna was run in polyacrylamide gel to determine the presence of rotaviral rna and the rest subjected to reverse transcriptase-polymerase chain reaction (rt-pcr) to generate the near full length vp7 gene. only six samples yielded the desired product. the amplified products were subjected to g-typing by pc ...200415861888
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