ectoparasitic and phoretic arthropods of virginia opossums (didelphis virginiana) in central tennessee.thirteen species of ectoparasitic (12) or phoretic (1) arthropods were collected from 26 adult virginia opossums, didelphis virginiana, live-trapped from april through september 1987 in davidson county, tennessee. the cat flea ctenocephalides felis and the american dog tick dermacentor variabilis were the predominant species with respect to mean intensity and prevalence. the macronyssid mite ornithonyssus wernecki and the atopomelid mite didelphilichus serrifer, both specific parasites of this h ...19902380870
seroprevalence of antibodies against toxoplasma gondii in free-ranging mammals in iowa.serum samples from raccoons (procyon lotor), striped skunks (mephitis mephitis), virginia opossums (didelphis virginiana), and free-ranging house cats trapped in iowa between 1984 and 1988 were tested for antibodies against toxoplasma gondii using the modified direct agglutination test (mat). antibody titers > or = 1:32 were considered indicative of infection. prevalence rates by species were estimated for raccoons at 134/885 (15%), skunks at 38/81 (47%), opossums at 12/53 (23%), and cats at 16/ ...19989813854
site selection by intestinal helminths of the virginia opossum (didelphis virginiana).digestive tract helminths of the virginia opossum (didelphis virginiana) were examined for site specificity and interspecific interactions. thirty opossums were live-trapped at 2 localities in georgia, killed, and their digestive tracts removed and divided into the following sections; esophagus, stomach, small intestine (20 equal sections), cecum, and large intestine (5 equal sections). helminths present in each section were identified and counted. three trematode, 1 cestode, 2 acanthocephala, a ...199910207354
patterns of infestation by adult dermacentor variabilis (acari: ixodidae) in a mark-recapture study of raccoons (mammalia: carnivora) and virginia opossums (mammalia: didelphimorphia) in tennessee.a mark-recapture study of raccoons (procyon lotor l.) and virginia opossums (didelphis virginiana kerr) was conducted from february 1991 through april 1994 to determine host interactions with adult dermacentor variabilis say. we captured 1,293 raccoons and virginia opossums during the 3-yr study (140 individual raccoons and 160 individual virginia opossums) with 1,895 adult d. variabilis collected. raccoons had a significantly higher mean intensity and higher prevalence of adult ticks than virgi ...199910337094
host utilization and seasonal occurrence of dermacentor species (acari:ixodidae) in missouri, usa.a total of 3,235 dermacentor variabilis (say) specimens were collected from birds, mammals, and by dragging vegetation, and 2,683 d. albipictus (packard) ticks were collected from deer from 1993 to 1996. peak seasonal occurrence of adult d. variabilis was from may through july with a precipitous decrease in august. nymphal d. variabilis populations peaked in june. peak activity of larvae was bimodal, with one activity peak during late summer (september) and a second peak in winter or early sprin ...200011201355
sporocyst size of isolates of sarcocystis shed by the virginia opossum (didelphis virginiana).the virginia opossum (didelphis virginiana) is a definitive host for multiple sarcocystis species including sarcocystis neurona, one of the causative agents of equine protozoal myeloencephalitis (epm), a severe, neuromuscular disease of horses. size and morphologic characteristics of isolates of sarcocystis shed by the opossum were examined to determine if differences were useful in discriminating between the isolates and/or species. collections of sporocysts from 17 opossums were molecularly ch ...200111223210
evaluation of the shedding of sarcocystis falcatula sporocysts in experimentally infected virginia opossums (didelphis virginiana).five virginia opossums (didelphis virginiana) were fed muscles of brown-headed cowbirds (molothrus ater) containing sarcocysts of sarcocystis falcatula. shedding of sporocysts was confirmed in all five opossums by fecal flotation. counts were conducted daily for 2 weeks and then biweekly until the animals were euthanized and necropsied. the average prepatent period was 9.8 (7-16) days. the number of sporocysts shed varied greatly between the opossums with maximum mean shedding occurring at 71.6 ...200111223211
spotted fever group rickettsia in dermacentor variabilis (acari: ixodidae) infesting raccoons (carnivora: procyonidae) and opossums (marsupialia: didelphimorphidae) in tennessee.spotted fever group rickettsia were present in 13 of 1,171 (1.1%) of adult dermacentor variabilis say removed from raccoons (procyon lotor l.) and virginia opossums (didelphis virginiana kerr) in western tennessee. spotted fever group rickettsia were detected by amplification of a 617-bp segment of the citrate synthase gene by polymerase chain reaction.200111476343
sarcocystis greineri n. sp. (protozoa: sarcocystidae) in the virginia opossum (didelphis virginiana).sarcocysts were found in the skeletal muscles of road-killed and live-trapped opossums collected in north central florida. sarcocysts were spindle-shaped and macroscopic and had an average measurement of 3.8 mm by 154.6 microm. sarcocysts were only observed in skeletal muscle. sarcocysts have invaginations throughout the sarcocyst wall, which is approximately 1 microm thick. protrusions on the sarcocyst wall are stumpy and digitlike and contain fibrillar elements that extend from the interior po ...200111695370
host associations and seasonal occurrence of haemaphysalis leporispalustris, ixodes brunneus, i. cookei, i. dentatus, and i. texanus (acari: ixodidae) in southeastern missouri.hemaphysalis leporispalustris (packard), ixodes brunneus koch, ixodes cookei packard, ixodes dentatus marx, and ixodes texanus banks were collected during a 3-yr study of pathogen-tick-host interactions in southeastern missouri. h. leporispalustris was collected from the eastern cottontail rabbit, northern bobwhite, and carolina wren, and it was active all year. i. brunneus was collected by drag and from passerine birds during december, march, and april. i. cookei was collected from raccoons and ...200312597662
comparison of the reservoir competence of medium-sized mammals and peromyscus leucopus for anaplasma phagocytophilum in the northeastern united states, anaplasma phagocytophilum, the agent of human granulocytic ehrlichiosis (hge), is transmitted by the tick vector ixodes scapularis. the white-footed mouse peromyscus leucopus is a competent reservoir for this agent, but the reservoir competence of non-peromyscus hosts of i. scapularis has not been studied. here, we report data confirming reservoir competence of medium-sized mammals for a. phagocytophilum. raccoons, virginia opossums, gray squirrels, and striped ...200212737542
prevalence and tissue distribution of besnoitia darlingi cysts in the virginia opossum (didelphis virginiana) in michigan.specimens of virginia opossums (didelphis virginiana) in michigan were examined over 1 year to document the presence of besnoitia darlingi cysts. cyst morphology, prevalence, seasonal variation, and tissue sites of isolation were studied. histology and ultrastructural features of the detected cysts and bradyzoites were consistent with b. darlingi. in the opossums, b. darlingi had intracellular tissue cysts. tissue cysts had a mean diameter of 560 microm and were separated from the host tissue by ...200312944045
serologic evidence of exposure of wild mammals to flaviviruses in the central and eastern united states.serosurveys were conducted to obtain flavivirus and west nile virus (wnv) seroprevalence data from mammals. sera from 513 small- and medium-sized mammals collected during late summer and fall 2003 from colorado, louisiana, new york, ohio, and pennsylvania were screened for flavivirus-specific antibodies. sera samples containing antibody to flaviviruses were screened for wnv-specific antibodies by epitope-blocking enzyme-linked immunosorbent assays and confirmed with plaque reduction neutralizati ...200515891139
bartonella and rickettsia in fleas and lice from mammals in south carolina, u.s.a.species in the genera bartonella and rickettsia are vector-borne pathogens of humans and domestic animals. the natural reservoirs and enzootic transmission cycles of these bacteria are poorly known in south carolina. thirteen species of lice and fleas were collected from urban animals and screened for the presence of bartonella and rickettsia by pcr amplification using genus-specific primers. bartonella henselae was present in cat fleas (ctenocephalides felis) from virginia opossums (didelphis v ...200516599169
serologic evidence of west nile virus exposure in north american mesopredators.sera from 936 mammalian mesopredators (virginia opossums, gray foxes, striped skunks, hooded skunks, raccoons, a bobcat, and a red fox) were collected during 2003 and 2004 in california, arizona, texas, louisiana, ohio, and wyoming and screened for flavivirus-specific antibodies by an epitope-blocking enzyme-linked immunosorbent assay (blocking elisa). serum samples positive for antibodies against flaviviruses were screened for west nile virus (wnv)-specific antibodies by blocking elisa and sele ...200717255248
observations on besnoitiosis in virginia opossum (didelphis virginiana) from michigan, usa.besnoitia tissue cysts were found in five naturally-infected adult opossums (didelphis virginiana) from michigan. details of the microscopy, histopathology, ultra-structure, and genetic features of the cysts were studied to identify their species-specific traits. the materials were differentiated phenotypically from cysts of other besnoitia spp. by difference in size, pattern of tissue distribution, morphology of pellicle and nucleus, number of micronemes and rhoptries, amount of lipids and amyl ...200717580564
diversity, seasonality, and context of mammalian roadkills in the southern great plains.thousands of mammals are killed annually from vehicle collisions, making the issue an important one for conservation biologists and environmental managers. we recorded all readily identifiable kills on or immediately adjacent to roads in the southern great plains from march 2004-march 2007. we also recorded distance traveled, whether a road was paved or divided, the number of lanes, and prevailing habitat. surveys were opportunistic and were conducted by car during conditions of good visibility. ...200818299918
ultrastructure characteristics of besnoitia darlingitachyzoites brumpt, 1913 (protozoa: sarcocystidae) of the michigan strain mibd1.tachyzoites of besnoitia darlingi brumpt, 1913 were redescribed based on new materials isolated from virginia opossums (didelphis virginiana, kerr) from michigan, u.s.a. tachyzoites of the mibd1 strain were propagated in bovine turbinate cell culture for more than two years. a comparison with previously described tachyzoites of the b. darlingi op1 strain from mississippi, usa revealed some morphological differences despite the remarkable genetic homogeneity between the two b. darlingi strains. m ...200718383798
evaluation of cruzia americana, turgida turgida, and didelphostrongylus hayesi infection in the virginia opossum (didelphis virginiana) and risk factors along the california coast.three nematodes, turgida turgida, cruzia americana, and didelphostrongylus hayesi, have been documented to cause morbidity and mortality in the virginia opossum (didelphis virginiana). the present study was designed to determine the frequency of infection of these nematodes in opossums at 2 study sites in california and to determine if there are risk factors associated with shedding of eggs or larvae in the feces. turgida turgida and c. americana adults were found in 84.4% (stomach; n = 45) and ...200818973414
ectoparasites and associated pathogens of free-roaming and captive animals in zoos of south carolina.a survey of ectoparasites and their associated pathogens was conducted in two south carolina zoos, from 2004 to 2007. dead, wild birds and mammals, as well as captive animals examined during routine veterinary checks constituted the study populations. ectoparasites were tested for species of anaplasma, bartonella, coxiella burnetii, ehrlichia, rickettsia, and trypanosoma. forty-six species of ectoparasites were collected from 133 free-roaming and captive hosts and their associated nesting and be ...200918973443
besnoitia darlingi infection in a virginia opossum (didelphis virginiana).this is a case report of natural infection with besnoitia darlingi in a virginia opossum (didelphis virginiana) in louisiana. clinical pathologic data included a severe nonregenerative anemia, inflammatory leukogram, increased hepatocellular leakage enzymes, renal azotemia, hyperkalemia, hypoglycemia, hypoalbuminemia, and proteinuria. tissue cysts containing bradyzoites were found in the majority of organs, especially the skin, mucous membranes, kidneys, adrenals, lungs, and heart. images of the ...200919368269
skeletal lesions in a population of virginia opossums (didelphis virginiana) from baldwin county, georgia.antemortem skeletal lesions were analyzed in 61 virginia opossums (didelphis virginiana) collected as roadkill from baldwin county, georgia, usa. as evidenced by bridging fracture calluses, misalignments, bone-surface depressions, perforations, localized superficial calluses, periosteal proliferations, and bony bridges across intervertebral disk spaces, 64% of the individuals had signs of one or more healed injuries. within the subset of skeletons with bone lesions, 54% had healed rib fractures ...200919395742
seasonal source-sink dynamics at the edge of a species' range.the roles of dispersal and population dynamics in determining species' range boundaries recently have received theoretical attention but little empirical work. here we provide data on survival, reproduction, and movement for a virginia opossum (didelphis virginiana) population at a local distributional edge in central massachusetts (usa). most juvenile females that apparently exploited anthropogenic resources survived their first winter, whereas those using adjacent natural resources died of sta ...200919569372
antibodies to west nile virus in raccoons and other wild peridomestic mammals in iowa.surveillance for evidence of west nile virus (wnv) infection in small- and medium-sized wild mammals was conducted in iowa, usa, from may 2005 to june 2007. sera were collected from 325 mammals belonging to nine species and tested for antibodies to wnv and other flaviviruses by epitope-blocking enzyme-linked immunosorbent assay (elisa). all sera that had antibodies to flaviviruses by blocking elisa were further examined by plaque reduction neutralization test (prnt). thirteen mammals were seropo ...200919901390
seroprevalence of trypanosoma cruzi among eleven potential reservoir species from six states across the southern united states.trypanosoma cruzi, the causative agent of chagas' disease, is a substantial public health concern in latin america. although rare in humans and domestic animals in the united states, t. cruzi is commonly detected in some wildlife species, most commonly raccoons (procyon lotor) and virginia opossums (didelphis virginiana). to increase our understanding of the reservoir host species range and geographic distribution, 11 species of mammals from six states spanning the known range of t. cruzi (arizo ...201020020815
serological response of cats to experimental besnoitia darlingi and besnoitia neotomofelis infections and prevalence of antibodies to these parasites in cats from virginia and pennsylvania.abstract besnoitia darlingi and besnoitia neotomofelis are cyst-forming tissue apicomplexan parasites that use domestic cats ( felis domesticus ) as definitive hosts and opossums ( didelphis virginiana ) and southern plains woodrats ( neotoma micropus ) as intermediate hosts, respectively. nothing is known about the prevalence of b. darlingi or b. neotomofelis in cats from the united states. besnoitia darlingi infections have been reported in naturally infected opossums from many states in the u ...201121506782
serological investigation of heartland virus (bunyaviridae: phlebovirus) exposure in wild and domestic animals adjacent to human case sites in missouri 2012-2013.heartland virus (hrtv; bunyaviridae: phlebovirus) has recently emerged as a causative agent of human disease characterized by thrombocytopenia and leukopenia in the united states. the lone star tick (amblyomma americanum l.) has been implicated as a vector. to identify candidate vertebrate amplification hosts associated with enzootic maintenance of the virus, sera and ticks were sampled from 160 mammals (8 species) and 139 birds (26 species) captured near 2 human case residences in andrew and no ...201525870419
west nile virus isolated from a virginia opossum (didelphis virginiana) in northwestern missouri, usa, 2012.we describe the isolation of west nile virus (wnv; flaviviridae, flavivirus) from blood of a virginia opossum (didelphis virginiana) collected in northwestern missouri, usa in august 2012. sequencing determined that the virus was related to lineage 1a wnv02 strains. we discuss the role of wildlife in wnv disease epidemiology.201425098303
surveillance and movements of virginia opossum (didelphis virginiana) in the bovine tuberculosis region of michigan.wildlife reservoir hosts of bovine tuberculosis (btb) include eurasian badgers (meles meles) and brushtail possum (trichosurus vulpecula) in the uk and new zealand, respectively. similar species warrant further investigation in the northern lower peninsula of michigan, usa due to the continued presence of btb on cattle farms. most research in michigan, usa has focused on interactions between white-tailed deer (odocoileus virginianus) and cattle (bos taurus) for the transmission of the infectious ...201323531427
viability of sarcocystis neurona sporocysts after long-term storage.the effect of long-term storage on the viability and infectivity of sarcocystis neurona sporocysts was investigated. s. neurona sporocysts were harvested from the small intestine of virginia opossums from 1996 to 2002 and stored at 4 degrees c. viability of sporocysts was assessed by propidium iodide (pi) exclusion assay, in vitro excystation and development in tissue cultures, and bioassay in gamma-interferon gene knockout (gamma-ifn-ko) mice. the rate of excystation was apparently unaffected b ...200415325051
amblyomma auricularium (ixodida: ixodidae) in florida: new hosts and distribution records.previous published evidence for the occurrence of an exotic armadillo tick, amblyomma auricularium (conil), in florida is scant, but we found it is fully established and integrated into the state's tick fauna. we collected 11,192 specimens of this tick from naturalized nine-banded armadillos, dasypus novemcinctus l., and 14 other species of wild native mammals and birds in florida, while sampling statewide during 2004 through 2007. in all, we document its specific presence only in 14 contiguous ...201728082640
evaluating wildlife-cattle contact rates to improve the understanding of dynamics of bovine tuberculosis transmission in michigan, and indirect contacts among individuals drive transmission of infectious disease. when multiple interacting species are susceptible to the same pathogen, risk assessment must include all potential host species. bovine tuberculosis (btb) is an example of a disease that can be transmitted among several wildlife species and to cattle, although the potential role of several wildlife species in spillback to cattle remains unclear. to better understand the complex network of contacts and factor ...201627931926
metal accumulation in bobcats in the southeastern usa.bobcats (lynx rufus) are wide-ranging mammals found throughout the continental usa. as carnivores near the top of their food chain, bobcats would seem to be a useful bioindicator of metal pollution in terrestrial environments. however, there is very limited research on bobcats in toxicology studies. here, we offer the first analysis of metal (copper, selenium, silver, and zinc) contaminants in the livers of wild bobcats. liver tissues from 120 adult bobcats (i.e., estimated to be ≥1 year old) we ...201627629555
metal accumulation in wild-caught opossum.the virginia opossum (didelphis virginiana) is widespread in the usa, ranging south through latin america. the ecology of opossums is such that they are in frequent contact with soils, suggesting that they may function as a valuable bioindicator for chemical contamination in terrestrial environments. surprisingly, there have been virtually no toxicology studies on opossums. here, we provide the first analysis of metal contaminants in opossum liver tissues. liver samples were obtained from 471 op ...201627138002
spontaneous neoplasms in captive virginia opossums ( didelphis virginiana): a retrospective case series (1989-2014) and review of the literature.this retrospective project summarizes the types of neoplasms identified in virginia opossums ( didelphis virginiana) presented to the university of tennessee, college of veterinary medicine (utcvm) postmortem service in 1989-2014 and serves as a review of the literature. of the 85 virginia opossums identified from the utcvm case database, there were 17 diagnoses of neoplasia from 12 cases (14%). these cases included 8 females, 2 males, and 2 neutered males. all opossums with known ages (11 of 12 ...201728375825
postmortem scavenging by the virginia opossum (didelphis virginiana): impact on taphonomic assemblages and progression.the virginia opossum (didelphis virginiana) is a highly active scavenger whose behavior has significant impacts on rates of decomposition and skeletonization, which have previously not been addressed. in this study, scavenging by the opossum led to the skeletonization of carcasses in half of the accumulated degree days (add) of a comparable non-scavenged control carcass. opossums used body orifices, as well as natural tears caused by the decomposition process, to access internal tissues and cons ...201627430919
carcass type affects local scavenger guilds more than habitat connectivity.scavengers and decomposers provide an important ecosystem service by removing carrion from the environment. scavenging and decomposition are known to be temperature-dependent, but less is known about other factors that might affect carrion removal. we conducted an experiment in which we manipulated combinations of patch connectivity and carcass type, and measured responses by local scavenger guilds along with aspects of carcass depletion. we conducted twelve, 1-month trials in which five raccoon ...201626886299
high prevalence of porocephalus crotali infection on a barrier island (cumberland island) off the coast of georgia, with identification of novel intermediate hosts.porocephalus crotali is a pentastomid parasite that uses crotaline snakes as definitive hosts and a variety of rodents as intermediate hosts. a study of definitive and intermediate pentastome hosts on cumberland island, georgia, revealed high prevalence of p. crotali infection in crotalid snakes as well as several mammalian species. despite the presence of numerous nymphs in some animals, clinical signs of disease were not observed. in intermediate hosts, the liver, mesentery, and reproductive o ...201526042344
a behaviorally-explicit approach for delivering vaccine baits to mesopredators to control epizootics in fragmented landscapes.despite the widespread use of aerial baiting to manage epizootics among free-ranging populations, particularly in rabies management, bait acceptance and seroconversion rates often are lower than required to eliminate spread of disease. our objectives in this study, therefore, were to evaluate the performance of stratified bait distribution models derived from resource selection functions (rsf) on uptake of placebo rabies baits by raccoons (procyon lotor) and virginia opossums (didelphis virginia ...201525587900
femoral loading mechanics in the virginia opossum, didelphis virginiana: torsion and mediolateral bending in mammalian locomotion.studies of limb bone loading in terrestrial mammals have typically found anteroposterior bending to be the primary loading regime, with torsion contributing minimally. however, previous studies have focused on large, cursorial eutherian species in which the limbs are held essentially upright. recent in vivo strain data from the virginia opossum (didelphis virginiana), a marsupial that uses a crouched rather than an upright limb posture, have indicated that its femur experiences appreciable torsi ...201121957109
in vivo strains in the femur of the virginia opossum (didelphis virginiana) during terrestrial locomotion: testing hypotheses of evolutionary shifts in mammalian bone loading and design.terrestrial locomotion can impose substantial loads on vertebrate limbs. previous studies have shown that limb bones from cursorial species of eutherian mammals experience high bending loads with minimal torsion, whereas the limb bones of non-avian reptiles (and amphibians) exhibit considerable torsion in addition to bending. it has been hypothesized that these differences in loading regime are related to the difference in limb posture between upright mammals and sprawling reptiles, and that the ...201121753057
prevalence of sarcocystis species sporocysts in northern virginia opossums (didelphis virginiana).a total of 206 virginia opossums ( didelphis virginiana) collected from the mid-michigan region, united states, during a period extending from 1996 to 2002 were sampled for the presence of sarcocystis spp sporocysts. all isolates were phenotypically identified as sarcocystis spp and genotyped to the species level by pcr-based techniques. the overall prevalence of sarcocystis spp in opossums was 18% (37/206). the prevalence of sarcocystis spp differed significantly with age ( p<0.001) and adult o ...200415205944
sarcocystis inghami n. sp. (sporozoa: sarcocystidae) from the skeletal muscles of the virginia opossum didelphis virginiana in michigan.this report describes the newly identified sarcocystis inghami n. sp. from the skeletal muscles of opossums (mammalia: didelphidae) that were collected from south central michigan (42 degrees 43'-42 degrees 79'n, 84 degrees 18'-84 degrees 86'w), usa. the new species is distinguished from all species described from north and south american opossums by the distinctive morphology of the villar protrusions on the cyst wall. sarcocysts of s. inghami are microscopic, up to 700 microm long and 110 micr ...200312975625
seasonal changes in the physiology of male virginia opossums (didelphis virginiana): signs of the dasyurid semelparity syndrome?semelparity, which is multiplying once in a lifetime, is a rare reproductive strategy among mammals. several species of the marsupial family dasyuridae experience 100% male mortality following an intense mating period. we investigated seasonal physiological changes that may be associated with early mortality in the male virginia opossum (didelphis virginiana; didelphidae) and compared these changes with those of semelparous, male dasyurids. free-ranging male virginia opossums (n=36) were collect ...201012905127
prevalence and site specificity of sarcocystis greineri sarcocysts in virginia opossum (didelphis virginiana) in florida.sarcocysts of sarcocystis greineri in the virginia opossum (didelphis virginiana) were observed for documenting sarcocyst prevalence, seasonal prevalence, and muscle specificity. characteristics of sarcocysts found in striated muscle were recorded, as were light microscopy measurements. overall prevalence of sarcocysts in striated muscle was 10.0% (24/240). no statistical difference (p = 0.156) in prevalence was detected between summer (13.1%; 16/122) and fall (6.7%; 8/118). sarcocysts were foun ...200212099438
scanning electron microscopy of turgida turgida (nematoda: spiruroidea), parasite of the virginia opossum, didelphis virginiana, from southern california.taxonomic characters for identification of turgida turgida rudolphi, 1819, a parasitic nematode of the virginia opossum didelphis virginiana, were studied by scanning electron microscopy. the distinguishing feature of the cephalic end is the presence of numerous denticles, structures associated with the internal tooth, and 2 spongelike areas on the inner side of each pseudolabia. the posterior end of male t. turgida differs from that in other species of physalopteridae by the number of caudal pa ...200111695400
diel temperature fluctuations of the virginia opossum (didelphis virginiana). 19734744938
characteristics of common helminths of the virginia opossum (didelphis virginiana) from north carolina. 19724336985
camera trap arrays improve detection probability of wildlife: investigating study design considerations using an empirical trapping is a standard tool in ecological research and wildlife conservation. study designs, particularly for small-bodied or cryptic wildlife species often attempt to boost low detection probabilities by using non-random camera placement or baited cameras, which may bias data, or incorrectly estimate detection and occupancy. we investigated the ability of non-baited, multi-camera arrays to increase detection probabilities of wildlife. study design components were evaluated for their infl ...201728422973
abiotic and biotic contributors to support inter-epidemic francisella tularensis in an agricultural peri-urban characterize the inter-epidemic ecology of francisella tularensis, we surveyed vertebrates and invertebrates for the abundance, spatial distribution, and status of infection at a site in northern california that had evidence of endemic type b tularemia. we collected 2910 mosquitoes, 77 biting flies, 704 ticks, 115 mammals, and 1911 aquatic invertebrates in 2013-2014. real-time pcr on all mosquitoes, 40 biting flies, 113 aquatic invertebrates, and 650 ticks did not detect f. tularensis dna. in ...201728968195
a genetic analysis of the virginia opossum mating system: evidence of multiple paternity in a highly fragmented landscape.using molecular techniques, we examined patterns of paternity in virginia opossums occupying a highly fragmented agricultural landscape in northern indiana. during 2008, we collected tissue from 64 females and their pouch young in 34 forest patches distributed over a 1100-km(2) region. using genotypes from 10 microsatellite loci, we determined the minimum number of fathers contributing to each litter using gerud 1.0. genotyped offspring with known mothers were then analyzed using cervus 3.0, inc ...201019995804
the ontogenesis of sleep states, thermogenesis, and thermoregulation in the virginia opossum.polygraphic recordings of sleep and wakefulness were obtained over 4-hr periods from infant opossums, aged 48-79 days, while they were in the mothers' pouches. concurrent body temperature measurements were obtained from littermates held at ambient temperatures of 25 and 30 degrees c. the ontogenesis of sleep was similar to that of placental mammals: quiet sleep increased from 13% sleep time at 48 days to 55% at 79 days; conversely, active sleep decreased from 87% to 45%, respectively. the amplit ...19807409325
age ratios, weights, and reproduction of the virginia opossum in north louisiana. 1978750645
taming of the virginia opossum. 196414110475
temperature regulation in the virginia opossum. 195514376572
the development of the cortico-spinal tract in the pouch-young of the virginia opossum. didelphys virginiana. 195413221669
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