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[isolation and characteristics of virus culture of porcine epidemic diarrhea virus ljb/03].porcine epidemic diarrhea virus (pedv) ljb/03 strain was isolated from the feces of piglets suspected to be suffering from a severe diarrhea in heilongjiang province, and was identified by immunofluorescence test, immunelectronmicroscopy, rt-pcr and indirect elisa assay. characteristics of the virus culture and the methods of improvement of virus titer were explored. the results showed that the virus had the typical appearance of the coronavirus. analysis of the nucleotide sequences of rt-pcr pr ...201021344754
cloning and sequence analysis of the m gene of porcine epidemic diarrhea virus ljb/03.porcine epidemic diarrhea virus (pedv) ljb/03 was isolated from the fece of piglets infected with pedv on a pig farm, heilongjiang province, china. the m gene of ljb/03 was amplified from the rna extracted directly from the fece samples by rt-pcr and cloned into pmd18-t vector. the m gene cdna was sequenced and encompasses an open reading frame of 681 nucleotides, encoding a 226-amino acid protein. the ljb/03 m gene has a base composition of 152 adenines (22%), 153 cytosines (23%), 161 guanines ...200515744564
a recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus.recently, porcine deltacoronavirus (pdcov) has been proven to be associated with enteric disease in piglets. diagnostic tools for serological surveys of pdcov remain in the developmental stage when compared with those for other porcine coronaviruses. in our study, an indirect enzyme-linked immunosorbent assay (elisa) (rpdcov-n-elisa) was developed to detect antibodies against pdcov using a histidine-tagged recombinant nucleocapsid (n) protein as an antigen. the rpdcov-n-elisa did not cross-react ...201626668175
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