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pcr-based detection of betanodaviruses from cultured and wild marine fish with no clinical signs.betanodaviruses are the causative agents of viral nervous necrosis (vnn) or viral encephalopathy and retinopathy (ver) in cultured marine fish. a total of 131 apparently healthy fish from 30 species were collected in two geographically remote aquaculture areas, yashima bay (kagawa prefecture) and tamanoura bay (nagasaki prefecture), in japan. the brains of fish were examined by reverse transcriptase-polymerase chain reaction (rt-pcr) and nested pcr to detect the coat protein gene of betanodaviru ...200415482425
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