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experimental challenge and clinical cases of bohle iridovirus (biv) in native australian anurans.ranaviruses have been observed with increasing frequency amongst poikilothermic vertebrate hosts. the impact of ranaviruses upon amphibian populations has remained largely unknown. a gene probe for bohle iridovirus (biv) based upon primers designed to detect epizootic haematopoietic necrosis virus (ehnv) was constructed. a pcr and dot-blot system was used successfully in screening for the presence of biv nucleic acid in digested formalin-fixed, paraffin-embedded amphibian tissues. juvenile frogs ...200212078986
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